Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,388,051	A→C	6.2%	S147A (TCC→GCC)	PP_1209 ←	cold‑shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,388,051	0	A	C	6.2%	200.9 / 3.0	63	S147A (TCC→GCC)	PP_1209	cold‑shock protein
Reads supporting (aligned to +/- strand):  ref base A (27/32);  new base C (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCACGGCCACGGC  >  NC_002947/1387904‑1388192                                                                                                                                                     |                                                                                                                                              ggTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGgag                                                                                                                                              <  4:23157/149‑1 (MQ=255)  gTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTTCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGgaga                                                                                                                                             <  6:10605/149‑1 (MQ=255)  gTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGgaga                                                                                                                                             <  2:34283/149‑1 (MQ=255)   tttACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGAt                                                                                                                                            <  6:3319/149‑1 (MQ=255)   tttACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGAt                                                                                                                                            <  4:70011/149‑1 (MQ=255)   tttACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGAt                                                                                                                                            <  8:161051/149‑1 (MQ=255)          cAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAAcc                                                                                                                                      >  3:229338/1‑148 (MQ=255)            aCCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTC‑CCGAGACTCGGGCGATGacatcttc                                                                                                                                   <  3:247537/149‑8 (MQ=255)            aCCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCaaa                                                                                                                                   <  5:310316/149‑1 (MQ=255)             ccACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAg                                                                                                                                  >  2:70312/1‑149 (MQ=255)             ccACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACACCACGCGATGCCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAg                                                                                                                                  >  8:187832/1‑149 (MQ=255)              cACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGc                                                                                                                                 >  5:258197/1‑149 (MQ=255)              cACGGCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTC‑CCGAGACTCGGGCGAt                                                                                                                                            >  4:219197/1‑138 (MQ=255)              cACGCCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTC‑CCGAGACTCGGGCGAt                                                                                                                                            <  3:219197/138‑1 (MQ=255)               aCGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGcc                                                                                                                                <  1:268346/149‑1 (MQ=255)                  tCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                             <  5:25552/149‑1 (MQ=255)                  tCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                             <  5:221594/149‑1 (MQ=255)                  tCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                             <  3:308346/149‑1 (MQ=255)                  tCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCtt                                                                                                                              >  4:231626/1‑148 (MQ=255)                     tCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGa                                                                                                                          >  4:21184/1‑149 (MQ=255)                      cGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTc                                                                                                                            <  7:152287/146‑1 (MQ=255)                             cAGGCCCTTGTCGCGGTGCAGCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGtt                                                                                                                    <  2:257109/147‑1 (MQ=255)                                  ccTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCAt                                                                                                             >  3:154921/1‑149 (MQ=255)                                       tCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGa                                                                                                         <  6:258197/148‑1 (MQ=255)                                         gcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACg                                                                                                       <  7:249612/148‑1 (MQ=255)                                                    ccGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCa                                                                                           >  5:310705/1‑149 (MQ=255)                                                    ccGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCa                                                                                           >  5:255960/1‑149 (MQ=255)                                                     cGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCAc                                                                                          >  7:80703/1‑149 (MQ=255)                                                      gAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACg                                                                                         <  3:107887/149‑1 (MQ=255)                                                        aaaCTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGt                                                                                       <  3:21184/149‑1 (MQ=255)                                                           cTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGc                                                                                    >  8:201343/1‑149 (MQ=255)                                                            tCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGcc                                                                                   >  5:273131/1‑149 (MQ=255)                                                               aCGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGACCCATTTGACGGTGCCGGTATCACGGTTGCCgg                                                                                 >  5:251000/1‑148 (MQ=255)                                                                    cTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCtt                                                                           <  2:293622/149‑1 (MQ=255)                                                                       gCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTtgtg                                                                        >  6:169887/1‑149 (MQ=255)                                                                       gCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTtgt                                                                         <  6:255960/148‑1 (MQ=255)                                                                            ctaCCAGATTGCGTTGGGGCTCACAGCGGATGGCCCGAAAGTGCAAGAAGATGTCTTCGG‑CCGAGTCTCGGGAGTTGATACGAAAGCGTTTCGACGTGTTGAACCATTTGACGGTGCAGGTATCAGGGTTGCCGGCATCTTGTGCCAc                                                                    <  4:229338/146‑1 (MQ=255)                                                                            cGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCAc                                                                    <  3:231626/148‑1 (MQ=255)                                                                             gACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACgt                                                                  >  5:316025/1‑149 (MQ=255)                                                                                 aGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTg                                                              <  4:49286/149‑1 (MQ=255)                                                                                  gAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGa                                                             <  6:30470/149‑1 (MQ=255)                                                                                  gAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGa                                                             <  4:101997/149‑1 (MQ=255)                                                                                    aTGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAAGCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCATGTGGCACGTTTTGAGt                                                           >  2:245390/1‑149 (MQ=255)                                                                                         gTGGCCCTCACCGCGTATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCcgc                                                      <  7:187832/149‑1 (MQ=255)                                                                                         gTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCAGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCcgc                                                      <  1:70312/149‑1 (MQ=255)                                                                                            gCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGt                                                    <  5:132087/148‑1 (MQ=255)                                                                                             cccTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGc                                                  <  5:169887/149‑1 (MQ=255)                                                                                              ccTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CAGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGcc                                                 >  2:189015/1‑149 (MQ=255)                                                                                                tCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCtt                                               >  4:12379/1‑149 (MQ=255)                                                                                                  aCCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgc                                             <  3:194702/149‑1 (MQ=255)                                                                                                   ccGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgcg                                            <  3:12379/149‑1 (MQ=255)                                                                                                      cgGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGt                                         <  6:316025/149‑1 (MQ=255)                                                                                                           ggCCCGAAAGTGCACGAAGATGTCATCGC‑GCGAGTCTCGGGAGATGAAACGAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGGGTGCCTTGCGCGtgctg                                     <  6:251000/148‑1 (MQ=255)                                                                                                             cccGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCATAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCgg                                  <  8:80703/149‑1 (MQ=255)                                                                                                              ccGAAAGTGCACGAAGATGTCATCGCGCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCgg                                  >  1:177767/1‑149 (MQ=255)                                                                                                                 aaaGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCaa                              <  6:128011/149‑1 (MQ=255)                                                                                                                        aCGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCAc                       >  8:58695/1‑149 (MQ=255)                                                                                                                          gAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACgg                     >  3:8477/1‑149 (MQ=255)                                                                                                                                gTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGAt               >  5:220777/1‑149 (MQ=255)                                                                                                                                 tCATCTC‑CCGAGACTCGGGCGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATg              >  7:35554/1‑149 (MQ=255)                                                                                                                                   aTCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGca            >  3:206863/1‑149 (MQ=255)                                                                                                                                       c‑ccGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggc        >  3:87868/1‑149 (MQ=255)                                                                                                                                         ccGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGGCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggcc       <  6:273131/149‑1 (MQ=255)                                                                                                                                         ccGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggc        >  3:317774/1‑148 (MQ=255)                                                                                                                                           gAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggccac     >  6:113499/1‑149 (MQ=255)                                                                                                                                                tcGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggccacggc  >  2:316459/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                              GGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGC‑CCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCACGGCCACGGC  >  NC_002947/1387904‑1388192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑