Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,412,899	C→T	20.7%	R195C (CGC→TGC)	PP_1234 →	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,412,899	0	C	T	20.7%	157.4 / 35.9	84	R195C (CGC→TGC)	PP_1234	permease
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base T (8/9);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-01

TGGGCGCTGACCTTCTCGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCAATTACGCAGCGCTATTGGCGTT  >  NC_002947/1412755‑1413047                                                                                                                                                 |                                                                                                                                                     tGGGCGCTGACCTTCTCGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCAc                                                                                                                                                  >  1:211585/1‑149 (MQ=255)   ggCGCGGACCTTCTCGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATTATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCAcc                                                                                                                                                 <  5:82760/148‑1 (MQ=255)          aCCTTCTCGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGaa                                                                                                                                         >  1:297168/1‑149 (MQ=255)            cTTCTCGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGaa                                                                                                                                         >  3:47733/1‑147 (MQ=255)             ttCTCGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAg                                                                                                                                      >  1:119930/1‑149 (MQ=255)               ctcGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAgg                                                                                                                                     <  3:74320/148‑1 (MQ=255)                tcGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGt                                                                                                                                   >  5:326144/1‑149 (MQ=255)                  gCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTgg                                                                                                                                 >  1:268488/1‑149 (MQ=255)                  gCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTg                                                                                                                                  <  4:47733/148‑1 (MQ=255)                  gCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTg                                                                                                                                  <  4:280284/148‑1 (MQ=255)                      tCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAg                                                                                                                             >  2:163344/1‑149 (MQ=255)                        cAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGc                                                                                                                            >  1:216771/1‑148 (MQ=255)                        cAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGc                                                                                                                            >  8:9347/1‑148 (MQ=255)                           ccTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCgaga                                                                                                                        <  1:338425/149‑1 (MQ=255)                              tggcaggctgGACAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGa                                                                                                                     <  4:196773/143‑1 (MQ=255)                                 aCTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAAcc                                                                                                                  >  5:168407/1‑149 (MQ=255)                                     ctgGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCgg                                                                                                                <  1:163344/147‑1 (MQ=255)                                     ctgGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCgg                                                                                                                <  7:9347/147‑1 (MQ=255)                                      tgGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCgg                                                                                                                >  4:22052/1‑146 (MQ=255)                                        gTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCgg                                                                                                                >  3:200086/1‑144 (MQ=255)                                           aaTGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGc                                                                                                        <  4:200086/149‑1 (MQ=255)                                            aTGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGcc                                                                                                       <  2:47505/149‑1 (MQ=255)                                                cATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACt                                                                                                   <  2:216771/149‑1 (MQ=255)                                                  tGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTAc                                                                                                 <  2:268488/149‑1 (MQ=255)                                                             gtgctggtgcCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGTAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTgg                                                                                         <  2:119930/146‑1 (MQ=255)                                                             gtgctggtgcCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTgg                                                                                         <  4:228383/146‑1 (MQ=255)                                                                   gtgcCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTAt                                                                                >  3:196822/1‑149 (MQ=255)                                                                   gtgcCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGt                                                                                  <  3:22052/147‑1 (MQ=255)                                                                    tgcCGATTCTGGTGTTCTTCTTCCTGTAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATc                                                                               <  6:326144/149‑1 (MQ=255)                                                                     gcCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATc                                                                               >  2:183906/1‑148 (MQ=255)                                                                      ccGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCg                                                                              <  2:211585/148‑1 (MQ=255)                                                                        gATTCTGGTGTTCTTCTTCCTAAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCgaga                                                                           <  1:42694/149‑1 (MQ=255)                                                                           tCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCTCCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTc                                                                        >  3:161100/1‑149 (MQ=255)                                                                            cTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTc                                                                        <  4:196822/148‑1 (MQ=255)                                                                               gtgtTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTc                                                                        <  6:125218/145‑1 (MQ=255)                                                                               gtgtTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTc                                                                        >  5:125218/1‑145 (MQ=255)                                                                               gtgtTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGa                                                                     <  6:168407/148‑1 (MQ=255)                                                                               gtgtTCTTCTTCCGGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGAGCACCTTGCTGAACAGCGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGa                                                                     <  6:98428/148‑1 (MQ=255)                                                                                 gtTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCt                                                                  >  7:2409/1‑149 (MQ=255)                                                                                 gtTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCt                                                                  >  6:8353/1‑149 (MQ=255)                                                                                    cttcttcCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGc                                                                <  5:8353/148‑1 (MQ=255)                                                                                     ttcttcCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGcc                                                                                                       <  8:278807/108‑1 (MQ=255)                                                                                     ttcttcCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGcc                                                                                                       >  7:278807/1‑108 (MQ=255)                                                                                     ttcttcCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCg                                                               >  3:3524/1‑148 (MQ=255)                                                                                      tcttcCTGAAGGATCGCGAACTCATT‑GCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTAGCTGAACAGGGTGGGAAGCGACATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCa                                                            >  3:259580/1‑149 (MQ=255)                                                                                       cttcCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCa                                                            >  3:265983/1‑149 (MQ=255)                                                                                       attcCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGAGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCa                                                            >  3:265982/2‑149 (MQ=255)                                                                                        ttcCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCAt                                                           >  7:313828/1‑149 (MQ=255)                                                                                          ccTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCAtt                                                          <  4:3524/148‑1 (MQ=255)                                                                                          ccTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCAtt                                                          <  2:297168/148‑1 (MQ=255)                                                                                           cTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGc                                                        >  5:153748/1‑149 (MQ=255)                                                                                           cTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGc                                                        >  2:80449/1‑149 (MQ=255)                                                                                            tGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGc                                                        >  7:263580/1‑148 (MQ=255)                                                                                              aaGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCAc                                                     >  8:149626/1‑149 (MQ=255)                                                                                                   tCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACa                                                >  7:229651/1‑149 (MQ=255)                                                                                                     gcgAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACAt                                               <  1:171911/148‑1 (MQ=255)                                                                                                      cgAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCg                                             >  7:266042/1‑149 (MQ=255)                                                                                                      cgAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCg                                             >  1:171681/1‑149 (MQ=255)                                                                                                       gAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGc                                            <  4:265982/149‑1 (MQ=255)                                                                                                       gAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGc                                            <  4:265983/149‑1 (MQ=255)                                                                                                         aCTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGcc                                           <  8:313828/148‑1 (MQ=255)                                                                                                           tCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCtt                                         >  1:192224/1‑148 (MQ=255)                                                                                                            cATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTtca                                       >  7:315646/1‑149 (MQ=255)                                                                                                             aTTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTtca                                       <  2:192224/148‑1 (MQ=255)                                                                                                             aTTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTCAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTtcat                                      <  2:2061/149‑1 (MQ=255)                                                                                                                 gCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGc                                  >  8:284476/1‑149 (MQ=255)                                                                                                                    gCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTtc                                        >  5:283531/1‑140 (MQ=255)                                                                                                                    gCTGGGTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTtc                                        <  6:283531/140‑1 (MQ=255)                                                                                                                    gCTGGGTCAGCGGCTACCTGCCGCGCCAGGGGACCTTGCTGAACAGGGTGGGCAGCGAGATGACCCGGCAGATCGCCAACTAGATCCGTGGCAAGTGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTg                               <  4:259580/149‑1 (MQ=255)                                                                                                                    gCTGGGTCAGCGGCTACCTGCCGCGCAAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGTGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTg                               <  8:118239/149‑1 (MQ=255)                                                                                                                     cTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTgg                              <  8:263580/149‑1 (MQ=255)                                                                                                                        ggTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGTGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTgggg                            <  1:80449/148‑1 (MQ=255)                                                                                                                        ggTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTgggg                            <  1:183906/148‑1 (MQ=255)                                                                                                                         gTCAGCGGCTACCTGCCGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCt                          <  8:315646/149‑1 (MQ=255)                                                                                                                         gTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCt                          <  7:149626/149‑1 (MQ=255)                                                                                                                          tCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCt                          >  6:69175/1‑148 (MQ=255)                                                                                                                            aGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCa                        <  3:168865/148‑1 (MQ=255)                                                                                                                            aGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGACAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCaa                       >  4:79417/1‑149 (MQ=255)                                                                                                                               ggCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAgg                                                                                    <  8:140341/85‑1 (MQ=255)                                                                                                                               ggCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAgg                                                                                    >  7:140341/1‑85 (MQ=255)                                                                                                                                     cTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCAATTACGCAgc              >  6:65353/1‑149 (MQ=255)                                                                                                                                      tGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCAATTACGCAgcg             >  5:289583/1‑149 (MQ=255)                                                                                                                                       gCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCAATTACGCAgcgc            >  8:71662/1‑149 (MQ=255)                                                                                                                                        ccGCGCCAGTGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCAATTACGCAGCGCt           <  8:2409/149‑1 (MQ=255)                                                                                                                                                 cgcACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCAATTACGCAGCGCTATTGGCGtt  <  5:65353/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     TGGGCGCTGACCTTCTCGCTGTCCAGCCTGCCACTGCTGGTCAATGCCATGATCTATCTGGTGCTGGTGCCGATTCTGGTGTTCTTCTTCCTGAAGGATCGCGAACTCATTGGCCGCTGGGTCAGCGGCTACCTGCCGCGCCAGCGCACCTTGCTGAACAGGGTGGGCAGCGAGATGAACCGGCAGATCGCCAACTACATCCGTGGCAAGGGTATCGAGATTCTGATCTGCGGCATTGCCACTTACATCGCCTTCATCAGCCTGGGGCTCAATTACGCAGCGCTATTGGCGTT  >  NC_002947/1412755‑1413047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑