Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,474,996 C→A 8.5% intergenic (‑776/+25) algD ← / ← PP_1289 GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,474,9960CA 8.5% 136.0 / 2.2 46intergenic (‑776/+25)algD/PP_1289GDP‑mannose 6‑dehydrogenase/DUF328 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (18/24);  new base A (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

AAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGA  >  NC_002947/1474849‑1475142
                                                                                                                                                   |                                                                                                                                                  
aaaaaaGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCa                                                                                                                                                   <  1:56978/149‑1 (MQ=255)
     aGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCcgc                                                                                                                                              <  5:284998/149‑1 (MQ=255)
        tATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGaa                                                                                                                                           <  8:247328/149‑1 (MQ=255)
              cGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                        <  4:268913/146‑1 (MQ=255)
              cGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTGAAGCCGCGAagag                                                                                                                                        >  3:268913/1‑146 (MQ=255)
               ggTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCg                                                                                                                                    <  5:189468/149‑1 (MQ=255)
                   ttATGGCGTTTGGCGATAACGGGAGGGGCGACGGATTTTTGGCGTCGGTTTCGTGGCGTGAGGGCGGTTTGCCGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGt                                                                                                                                   <  4:21201/146‑1 (MQ=255)
                   ttATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGag                                                                                                                                >  3:21201/1‑148 (MQ=255)
                                 gATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCcgcg                                                                                                                  >  8:253166/1‑149 (MQ=255)
                                  aTAAAGGGAGGTGCGACTGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCgg                                                                                                                 <  2:215540/149‑1 (MQ=255)
                                           ggggCGACGGATTTTTGGCGTCGGATGCGTGGCGTGATGGCGGGTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATcgc                                                                                                        <  3:261130/146‑1 (MQ=255)
                                               cGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAg                                                                                                    >  3:63133/1‑149 (MQ=255)
                                                  cGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGaa                                                                                                 <  5:253999/149‑1 (MQ=255)
                                                          tGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCaaa                                                                                          <  7:253166/148‑1 (MQ=255)
                                                           ggCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAAt                                                                                         <  3:289756/148‑1 (MQ=255)
                                                               tCGGATTATTGGCTTGATGGCGGTTTGAAGCGTGCCCTTTTGCAGGGCAGAGTCGGATCAAGGCTGCTGACCTCTTATCGGCTTCAGCCGAGAAGAGGGCGTGACATGATCACTCAGCGGCGTGATAGCGCAGGAACACCAAATGGTCt                                                                                    >  1:76691/1‑149 (MQ=255)
                                                                     tCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                              >  6:251738/1‑149 (MQ=255)
                                                                      cGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTg                                                                              <  1:188794/148‑1 (MQ=255)
                                                                             tGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTc                                                                      >  6:312774/1‑149 (MQ=255)
                                                                               aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTgg                                                                                  <  5:35450/135‑1 (MQ=255)
                                                                               aTGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTgg                                                                                  >  6:35450/1‑135 (MQ=255)
                                                                                  gCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCAc                                                                 >  8:307690/1‑149 (MQ=255)
                                                                                     gTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgt                                                              <  3:110077/149‑1 (MQ=255)
                                                                                      tttGCAGCGTGCCCTGTTGCATGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGc                                                                                 <  6:95446/129‑1 (MQ=255)
                                                                                        gccAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCCGACCGCTTCGCGGCGTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATCGTCTGGCTTGGACTGCTCGGCACTgtag                                                            <  8:290764/146‑1 (MQ=255)
                                                                                          cAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCCCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtagta                                                          <  7:102312/148‑1 (MQ=255)
                                                                                          cAGCGTGACCTCTTTCAGGTCATAGGCGGAGCACGGCGGGTGACTTCTTGGCGTCTTAAGCATCGAATAGTTCGTTACATGCTCACTCCGCGGTGTGATCGCGCAGGAACACCAAATGTTCTGGATTTGACTGCTCTTCACTttatta                                                          <  2:76691/148‑1 (MQ=255)
                                                                                             cGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTAc                                                      <  5:262084/149‑1 (MQ=255)
                                                                                                        gCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGTGTTATCGCTCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCAt                                            <  7:305222/148‑1 (MQ=255)
                                                                                                          aGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtag                                                            >  7:6296/1‑130 (MQ=255)
                                                                                                          aGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtag                                                            <  8:6296/130‑1 (MQ=255)
                                                                                                          aGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtag                                                            <  6:308337/130‑1 (MQ=255)
                                                                                                          aGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTgtag                                                            >  5:308337/1‑130 (MQ=255)
                                                                                                           gggCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGaa                                        >  6:111628/1‑149 (MQ=255)
                                                                                                              cAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTg                                     >  1:175842/1‑149 (MQ=255)
                                                                                                                gagGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGAGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCAGAGCATCGACCTGCt                                   >  8:133228/1‑149 (MQ=255)
                                                                                                                      gATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCt                             <  5:251738/149‑1 (MQ=255)
                                                                                                                        tCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGt                           >  2:278998/1‑149 (MQ=255)
                                                                                                                          aCGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTc                         >  6:129542/1‑149 (MQ=255)
                                                                                                                            ggcggcTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCg                       <  7:307690/149‑1 (MQ=255)
                                                                                                                              cggcTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCggg                     >  6:268993/1‑149 (MQ=255)
                                                                                                                                 cTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTg                  <  5:129542/149‑1 (MQ=255)
                                                                                                                                   gcccTCTTCGCGGCTGAAGCCGCGAAGAGGTCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGACCTGTTCCGGGGTGCt                >  8:288464/3‑149 (MQ=255)
                                                                                                                                    aCCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCt                <  5:312774/148‑1 (MQ=255)
                                                                                                                                       tctTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTACGGGGTGCTGATg            >  3:274683/1‑149 (MQ=255)
                                                                                                                                                 ttCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGa  >  6:12048/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAAAAAGGTATGAACGGTCTTATGGCGTTTGGCGATAAAGGGAGGTGCGACGGATTTTTGGCGTCGGATTCGTGGCGTGATGGCGGTTTGCAGCGTGCCCTGTTGCAGGGCAGAGGCGGATCACGGCGGCTGACCTCTTCGCGGCTTCAGCCGCGAAGAGGGCGTGACAGGCTCACTCCGCGGGGTGATCGCGCAGGAACACCAAATGGTCTGGCTTGGACTGCTCGGCACTGTAGTAGTACCCCTGAGCATCGAACTGCTTGAGCTGTTCCGGGGTGCTGATGCGCTGCTGGA  >  NC_002947/1474849‑1475142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑