Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,496,381	T→G	5.7%	C270W (TGT→TGG)	mdeA →	methionine gamma‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,496,381	0	T	G	5.7%	220.0 / 2.1	69	C270W (TGT→TGG)	mdeA	methionine gamma‑lyase
Reads supporting (aligned to +/- strand):  ref base T (30/35);  new base G (2/2);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCGGGTTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAGG  >  NC_002947/1496233‑1496529                                                                                                                                                      |                                                                                                                                                     ccGGGGTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACTCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGCCCGGCAgtgt                                                                                                                                                      >  8:273870/1‑149 (MQ=255)  cGGGTTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGTGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATtgt                                                                                                                                                      <  1:88726/149‑1 (MQ=255)         gtggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGc                                                                                                                                              >  4:168534/1‑149 (MQ=255)         gtggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGc                                                                                                                                              <  2:70668/149‑1 (MQ=255)          tggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGcc                                                                                                                                             >  6:122722/1‑149 (MQ=255)          tggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGcc                                                                                                                                             <  4:165170/149‑1 (MQ=255)               gggCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCcagca                                                                                                                                        <  6:77724/149‑1 (MQ=255)                 gcgcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATg                                                                                                                                               >  6:230899/1‑140 (MQ=255)                 gcgcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATg                                                                                                                                               <  5:230899/140‑1 (MQ=255)                   gcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccggtcc                                                                                                                                        >  1:207847/1‑140 (MQ=255)                   gcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccggtcc                                                                                                                                        <  2:207847/145‑6 (MQ=255)                    cAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTgg                                                                                                                                   >  4:273943/1‑149 (MQ=255)                        gtgtTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGa                                                                                                                               <  7:55698/149‑1 (MQ=255)                               tCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGc                                                                                                                                                    <  2:96209/121‑1 (MQ=255)                               tCGATCGCATTCGCCTGGAAGGGCTGAAGGATAAGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGc                                                                                                                                                    >  1:96209/1‑121 (MQ=255)                                 gATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATtgtg                                                                                                                                                     <  3:163414/118‑1 (MQ=255)                                 gATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATtgtg                                                                                                                                                     >  4:163414/1‑118 (MQ=255)                                  aTCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTc                                                                                                                     >  4:173661/1‑149 (MQ=255)                                      cATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCgg                                                                                                                  >  5:124612/1‑148 (MQ=255)                                             cTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCg                                                                                                            >  7:141142/1‑147 (MQ=255)                                                 aatggCTGAATGATATGACCGGGTCAGTGCTGTCGCCCCATGACTCG‑GTGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTg                                                                                                      <  8:206046/146‑1 (MQ=255)                                                   gggCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCg                                                                                                                   <  6:326542/134‑1 (MQ=255)                                                   gggCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCg                                                                                                                   >  5:326542/1‑134 (MQ=255)                                                     gCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAAt                                                                                                  <  5:122722/149‑1 (MQ=255)                                                      cTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAAtt                                                                                                 >  1:6332/1‑149 (MQ=255)                                                       tGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAAtt                                                                                                 >  4:161778/1‑148 (MQ=255)                                                         aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      >  6:120600/1‑125 (MQ=255)                                                         aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      <  5:120600/125‑1 (MQ=255)                                                         aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTg                                                                                                <  6:189009/147‑1 (MQ=255)                                                           ggATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            <  3:273943/149‑1 (MQ=255)                                                           ggATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            <  6:174308/149‑1 (MQ=255)                                                             atatGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCAt                                                                                          >  4:256680/1‑149 (MQ=255)                                                                tGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTa                                                                                        <  8:169126/148‑1 (MQ=255)                                                                     ggggCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCggg                                                                                  <  6:261976/149‑1 (MQ=255)                                                                        gCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGc                                                                                >  4:296312/1‑148 (MQ=255)                                                                          aGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTAc                                                                             >  6:79252/1‑149 (MQ=255)                                                                            tGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCg                                                                           >  4:324728/1‑149 (MQ=255)                                                                              cTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTc                                                                         >  3:83800/1‑149 (MQ=255)                                                                                gTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCtt                                                                       >  1:113177/1‑149 (MQ=255)                                                                                   gCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCtttt                                                                     <  3:161778/148‑1 (MQ=255)                                                                                   gCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCtttt                                                                     <  3:168534/148‑1 (MQ=255)                                                                                    ccccATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGc                                                                   >  5:202099/1‑149 (MQ=255)                                                                                      ccATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGAAGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGcc                                                                  >  1:50419/1‑148 (MQ=255)                                                                                        aTGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAg                                                               <  4:83800/149‑1 (MQ=255)                                                                                           aCGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCTGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTa                                                             <  3:296312/148‑1 (MQ=255)                                                                                             gcg‑gcgCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTAcgc                                                          <  3:173661/149‑1 (MQ=255)                                                                                               g‑gcgCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCg                                                                                    >  3:274554/1‑121 (MQ=255)                                                                                               g‑gcgCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCg                                                                                    <  4:274554/121‑1 (MQ=255)                                                                                                 gcgcTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGtt                                                       <  6:124612/149‑1 (MQ=255)                                                                                                   gctgctGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGt                                                        >  8:20579/1‑146 (MQ=255)                                                                                                   gctgctGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGt                                                        <  7:20579/146‑1 (MQ=255)                                                                                                        tgATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAg                                                >  6:79714/1‑149 (MQ=255)                                                                                                             cgcgGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACa                                           <  2:50419/149‑1 (MQ=255)                                                                                                              gcgGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGa                                             >  5:32986/1‑146 (MQ=255)                                                                                                              gcgGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGa                                             >  7:128877/1‑146 (MQ=255)                                                                                                                  gATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      <  3:133398/69‑1 (MQ=255)                                                                                                                  gATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      >  4:133398/1‑69 (MQ=255)                                                                                                                        gACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGc                                <  1:277936/149‑1 (MQ=255)                                                                                                                          cGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGTCAGATGCGTTTGCCt                              <  2:113177/149‑1 (MQ=255)                                                                                                                               gccttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            <  6:258793/82‑1 (MQ=255)                                                                                                                               gccttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            >  5:258793/1‑82 (MQ=255)                                                                                                                               gccttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGc                           <  5:79252/147‑1 (MQ=255)                                                                                                                                 cttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGcc                                                   >  1:54420/1‑121 (MQ=255)                                                                                                                                 cttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGcc                                                   <  2:54420/121‑1 (MQ=255)                                                                                                                                      gcATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGt                                                        <  1:663/111‑1 (MQ=255)                                                                                                                                      gcATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTATTTTGCCAAGTACGAGt                                                        >  2:663/1‑111 (MQ=255)                                                                                                                                       cATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTg                 <  5:79714/149‑1 (MQ=255)                                                                                                                                        aTGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGAtt                  <  5:141383/147‑1 (MQ=255)                                                                                                                                        aTGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGAtt                  >  6:141383/1‑147 (MQ=255)                                                                                                                                            aCCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCttt            <  3:324728/149‑1 (MQ=255)                                                                                                                                             tgggCATTGGCACAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCtt             <  1:33983/145‑1 (MQ=255)                                                                                                                                             tgggCATTGGCACAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCtt             >  2:33983/3‑147 (MQ=255)                                                                                                                                              cGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGa          <  6:202099/149‑1 (MQ=255)                                                                                                                                                      tgCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCGAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAgg  >  1:324568/1‑149 (MQ=255)                                                                                                                                                      tgCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAgg  <  8:141142/149‑1 (MQ=255)                                                                                                                                                      tgCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAg   <  3:256680/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CCGGGTTGGTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCG‑GCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAGG  >  NC_002947/1496233‑1496529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑