Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,496,383	C→A	5.7%	A271D (GCC→GAC) ‡	mdeA →	methionine gamma‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,496,383	0	C	A	5.7%	225.1 / 3.6	70	A271D (GCC→GAC) ‡	mdeA	methionine gamma‑lyase
Reads supporting (aligned to +/- strand):  ref base C (30/36);  new base A (2/2);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

GTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAGG  >  NC_002947/1496241‑1496529                                                                                                                                               |                                                                                                                                                   gtggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGc                                                                                                                                              <  2:70668/149‑1 (MQ=255) gtggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGc                                                                                                                                              >  4:168534/1‑149 (MQ=255)  tggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGcc                                                                                                                                             <  4:165170/149‑1 (MQ=255)  tggtggGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGcc                                                                                                                                             >  6:122722/1‑149 (MQ=255)       gggCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCcagca                                                                                                                                        <  6:77724/149‑1 (MQ=255)         gcgcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATg                                                                                                                                               >  6:230899/1‑140 (MQ=255)         gcgcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATg                                                                                                                                               <  5:230899/140‑1 (MQ=255)           gcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccggtcc                                                                                                                                        >  1:207847/1‑140 (MQ=255)           gcAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGGCACAATGccggtcc                                                                                                                                        <  2:207847/145‑6 (MQ=255)            cAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTgg                                                                                                                                   >  4:273943/1‑149 (MQ=255)                gtgtTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGa                                                                                                                               <  7:55698/149‑1 (MQ=255)                       tCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGc                                                                                                                                                    <  2:96209/121‑1 (MQ=255)                       tCGATCGCATTCGCCTGGAAGGGCTGAAGGATAAGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGc                                                                                                                                                    >  1:96209/1‑121 (MQ=255)                          aTCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTc                                                                                                                     >  4:173661/1‑149 (MQ=255)                              cATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCgg                                                                                                                  >  5:124612/1‑148 (MQ=255)                                     cTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCg                                                                                                            >  7:141142/1‑147 (MQ=255)                                         aatggCTGAATGATATGACCGGGTCAGTGCTGTCGCCCCATGACTCGGTGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTg                                                                                                      <  8:206046/146‑1 (MQ=255)                                           gggCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCg                                                                                                                   <  6:326542/134‑1 (MQ=255)                                           gggCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCg                                                                                                                   >  5:326542/1‑134 (MQ=255)                                             gCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAAt                                                                                                  <  5:122722/149‑1 (MQ=255)                                              cTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAAtt                                                                                                 >  1:6332/1‑149 (MQ=255)                                               tGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAAtt                                                                                                 >  4:161778/1‑148 (MQ=255)                                                 aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      <  5:120600/125‑1 (MQ=255)                                                 aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      >  6:120600/1‑125 (MQ=255)                                                 aaGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTg                                                                                                <  6:189009/147‑1 (MQ=255)                                                   ggATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            <  3:273943/149‑1 (MQ=255)                                                   ggATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            <  6:174308/149‑1 (MQ=255)                                                     atatGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCAt                                                                                          >  4:256680/1‑149 (MQ=255)                                                        tGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTa                                                                                        <  8:169126/148‑1 (MQ=255)                                                             ggggCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCggg                                                                                  <  6:261976/149‑1 (MQ=255)                                                                gCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGc                                                                                >  4:296312/1‑148 (MQ=255)                                                                  aGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTAc                                                                             >  6:79252/1‑149 (MQ=255)                                                                    tGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCg                                                                           >  4:324728/1‑149 (MQ=255)                                                                      cTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTc                                                                         >  3:83800/1‑149 (MQ=255)                                                                        gTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCtt                                                                       >  1:113177/1‑149 (MQ=255)                                                                           gCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCtttt                                                                     <  3:161778/148‑1 (MQ=255)                                                                           gCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCtttt                                                                     <  3:168534/148‑1 (MQ=255)                                                                            ccccATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGc                                                                   >  5:202099/1‑149 (MQ=255)                                                                              ccATGACGCGGCGCTGCTGATGCGCGGGATCAAGAAGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGcc                                                                  >  1:50419/1‑148 (MQ=255)                                                                                aTGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAg                                                               <  4:83800/149‑1 (MQ=255)                                                                                   aCGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCTGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTa                                                             <  3:296312/148‑1 (MQ=255)                                                                                     gcggcgCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTAcgc                                                          <  3:173661/149‑1 (MQ=255)                                                                                       ggcgCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCg                                                                                    <  4:274554/121‑1 (MQ=255)                                                                                       ggcgCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCg                                                                                    >  3:274554/1‑121 (MQ=255)                                                                                        gcgcTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGtt                                                       <  6:124612/149‑1 (MQ=255)                                                                                          gctgctGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGt                                                        >  8:20579/1‑146 (MQ=255)                                                                                          gctgctGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGt                                                        <  7:20579/146‑1 (MQ=255)                                                                                               tgATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAg                                                >  6:79714/1‑149 (MQ=255)                                                                                                    cgcgGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACa                                           <  2:50419/149‑1 (MQ=255)                                                                                                     gcgGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGa                                             >  5:32986/1‑146 (MQ=255)                                                                                                     gcgGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGa                                             >  7:128877/1‑146 (MQ=255)                                                                                                         gATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      >  4:133398/1‑69 (MQ=255)                                                                                                         gATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGt                                                                                                                      <  3:133398/69‑1 (MQ=255)                                                                                                               gACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGc                                <  1:277936/149‑1 (MQ=255)                                                                                                                 cGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGTCAGATGCGTTTGCCt                              <  2:113177/149‑1 (MQ=255)                                                                                                                      gccttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            >  5:258793/1‑82 (MQ=255)                                                                                                                      gccttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTc                                                                                            <  6:258793/82‑1 (MQ=255)                                                                                                                      gccttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGc                           <  5:79252/147‑1 (MQ=255)                                                                                                                        cttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGcc                                                   >  1:54420/1‑121 (MQ=255)                                                                                                                        cttgcGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGcc                                                   <  2:54420/121‑1 (MQ=255)                                                                                                                             gcATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGt                                                        <  1:663/111‑1 (MQ=255)                                                                                                                             gcATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTATTTTGCCAAGTACGAGt                                                        >  2:663/1‑111 (MQ=255)                                                                                                                              cATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTg                 <  5:79714/149‑1 (MQ=255)                                                                                                                               aTGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGAtt                  >  6:141383/1‑147 (MQ=255)                                                                                                                               aTGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGAtt                  <  5:141383/147‑1 (MQ=255)                                                                                                                                   aCCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCttt            <  3:324728/149‑1 (MQ=255)                                                                                                                                    tgggCATTGGCACAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCtt             <  1:33983/145‑1 (MQ=255)                                                                                                                                    tgggCATTGGCACAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCtt             >  2:33983/3‑147 (MQ=255)                                                                                                                                     cGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGa          <  6:202099/149‑1 (MQ=255)                                                                                                                                             tgCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCGAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAgg  >  1:324568/1‑149 (MQ=255)                                                                                                                                             tgCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAgg  <  8:141142/149‑1 (MQ=255)                                                                                                                                             tgCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAg   <  3:256680/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   GTGGTGGGGCGCAAGGTGTTGGTCGATCGCATTCGCCTGGAAGGGCTGAAGGATATGACCGGGGCAGTGCTGTCGCCCCATGACGCGGCGCTGCTGATGCGCGGGATCAAGACGCTTGCCTTGCGCATGGACCGGCATTGTGCCAATGCCCAGCAGGTGGCGGAGTTTCTCGTCCGGCAGCCGCAGGTGGAATTGATTCATTACCCGGGGCTACCGTCTTTTGCCCAGTACGCGTTGGCCCAGCGACAGATGCGTTTGCCTGGCGGGATGATTGCCTTTGAGCTCAAGG  >  NC_002947/1496241‑1496529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑