Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,594,066 G→T 7.4% intergenic (‑264/+37) PP_1399 ← / ← kgtP hypothetical protein/alpha‑ketoglutarate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,594,0660GT 7.4% 167.9 / 4.0 54intergenic (‑264/+37)PP_1399/kgtPhypothetical protein/alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base T (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01

CGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAA  >  NC_002947/1593923‑1594198
                                                                                                                                               |                                                                                                                                    
cgcgTATGTCGTGCTTCAGGCCGCAATGCTGGCTTGGGAGCGTTCGCGCGTGCGCCAACGTTCTGCCAGCGATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGATTGAAAAGTCTGAATAGAAAATGGGCGCTGCATGCAATg                                                                                                                                 <  2:203225/149‑1 (MQ=255)
  cgTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATggg                                                                                                                               <  2:90612/149‑1 (MQ=255)
       gTCGTGCTTCAGGCCGCAAGGCTGGATTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGcc                                                                                                                          >  7:3639/1‑149 (MQ=255)
        tCGTGCTTCAGGCCGCAAGGCTGGATTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGcc                                                                                                                          <  8:3639/148‑1 (MQ=255)
          gTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCt                                                                                                                       >  3:86956/1‑149 (MQ=255)
            gCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                     >  7:269343/1‑149 (MQ=255)
              ttCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCttt                                                                                                                     >  4:231101/1‑147 (MQ=255)
              ttCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTa                                                                                                                   >  8:10681/1‑149 (MQ=255)
                cAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGcc                                                                                                                          >  1:116033/1‑140 (MQ=255)
                cAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAGCGcc                                                                                                                          <  2:116033/140‑1 (MQ=255)
                   gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGATTAGAAAAGGGGCGCTCCCTGCAATGGGGCGCCCCTTTTAGTACt                                                                                                              >  5:229471/1‑149 (MQ=255)
                   gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACt                                                                                                              >  6:92044/1‑149 (MQ=255)
                   gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAgcgc                                                                                                                           <  5:115116/136‑1 (MQ=255)
                   gCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCCCCATTGCATGGAgcgc                                                                                                                           >  6:115116/1‑136 (MQ=255)
                     cGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgc                                                                                                            <  3:21701/149‑1 (MQ=255)
                     cGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgc                                                                                                            >  8:220407/1‑149 (MQ=255)
                       cAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgcca                                                                                                          <  8:152963/149‑1 (MQ=255)
                        aaGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccag                                                                                                         <  5:70071/149‑1 (MQ=255)
                          ggctggctTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccaggc                                                                                                       <  8:290604/149‑1 (MQ=255)
                          ggctggctTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCA‑TGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccaggcc                                                                                                      >  4:102363/1‑149 (MQ=255)
                                     gAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACt                                                                                             >  5:154455/1‑148 (MQ=255)
                                       gCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAg                                                                                          <  5:92044/149‑1 (MQ=255)
                                         gTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTg                                                                                        <  3:231101/149‑1 (MQ=255)
                                               gcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATc                                                                                  >  3:5705/1‑149 (MQ=255)
                                                        aaCGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgc                                                                         >  1:175288/1‑149 (MQ=255)
                                                           gTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgctgc                                                                      >  6:242524/1‑149 (MQ=255)
                                                           gTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCAGCGAGtgctgc                                                                      >  1:317359/1‑149 (MQ=255)
                                                                 ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCTTGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGgt                                                                 <  5:246473/148‑1 (MQ=255)
                                                                 ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                                                                   <  2:175288/146‑1 (MQ=255)
                                                                 ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                                                                   <  8:269343/146‑1 (MQ=255)
                                                                 ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGgt                                                                 <  8:54104/148‑1 (MQ=255)
                                                                       aTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCttt                                                           >  5:284595/1‑148 (MQ=255)
                                                                           cAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGAAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGt                                                                           <  3:63225/128‑1 (MQ=255)
                                                                           cAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGAAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGt                                                                           >  4:63225/1‑128 (MQ=255)
                                                                             gCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTc                                                    <  6:284595/149‑1 (MQ=255)
                                                                                     aGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTaaa                                             <  4:11475/148‑1 (MQ=255)
                                                                                       gTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAAc                                            >  3:180197/1‑147 (MQ=255)
                                                                                           aaTTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACcagcag                                      <  6:154455/149‑1 (MQ=255)
                                                                                            aTTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTTATGGTCGCGTAAACCagcagc                                     <  4:5705/149‑1 (MQ=255)
                                                                                               aaTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAg                                  >  7:237298/1‑149 (MQ=255)
                                                                                                aTAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGc                                 <  4:86956/149‑1 (MQ=255)
                                                                                                 tAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCa                                <  7:220407/149‑1 (MQ=255)
                                                                                                   gggCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAg                               <  8:145931/148‑1 (MQ=255)
                                                                                                    ggCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGc                             >  2:131032/1‑149 (MQ=255)
                                                                                                    ggCGTTAAGAAGGAAAAGTCTGAATAGAAAAGCGCCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGc                             <  6:87844/149‑1 (MQ=255)
                                                                                                      cGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGa                           <  8:237298/149‑1 (MQ=255)
                                                                                                          aagaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATa                         >  4:252488/1‑147 (MQ=255)
                                                                                                           agaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAg                      >  5:151711/1‑149 (MQ=255)
                                                                                                            gaagGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAgg                     <  1:131032/149‑1 (MQ=255)
                                                                                                                 aaaaGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGt                 >  7:201829/1‑148 (MQ=255)
                                                                                                                          aaTAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTAc          <  4:180197/146‑1 (MQ=255)
                                                                                                                                aaaGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGaa  <  7:10681/148‑1 (MQ=255)
                                                                                                                                            cATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                                                                   <  4:220719/71‑1 (MQ=255)
                                                                                                                                            cATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                                                                   >  3:220719/1‑71 (MQ=255)
                                                                                                                                               |                                                                                                                                    
CGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAA  >  NC_002947/1593923‑1594198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑