Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,647,927 G→A 100% Y265Y (TAC→TAT)  oprB‑II ← carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,647,9270GA100.0% 132.8 / NA 41Y265Y (TAC→TAT) oprB‑IIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/20);  total (21/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCG  >  NC_002947/1647783‑1648068
                                                                                                                                                |                                                                                                                                             
gTCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTgtaatag                                                                                                                                            <  2:93443/148‑1 (MQ=255)
                gTGACCTCCTGCTGGGCGCCAATCCACATGCCGTGCTTGCTCGACCTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTTAGAACATCTTGTGCCTTGTCATTACTGTAATAGTAGCCGGCACGATATTc                                                                                                                           <  7:51499/149‑1 (MQ=255)
                     aagctgcagGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGGTGCTGTCCTTGAGAACATCTTGTGCCTTGGGATTACTGTAATAGTAGCCGGCACGATATTCCCCtt                                                                                                                      >  2:159162/3‑149 (MQ=255)
                              ggCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                             <  1:292669/149‑1 (MQ=255)
                              ggCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCtt                                                                                                             <  3:259247/149‑1 (MQ=255)
                                   cAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTAGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATa                                                                                                        >  6:229010/1‑149 (MQ=255)
                                    aaTCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATAc                                                                                                       >  2:112387/1‑149 (MQ=255)
                                     aTCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACg                                                                                                      >  7:66056/1‑149 (MQ=255)
                                       ccACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTg                                                                                                    >  7:254078/1‑149 (MQ=255)
                                        cacaAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGTACGATATTCCCCTTTCAAGCCTTGGATACGTg                                                                                                    <  5:21904/148‑1 (MQ=255)
                                                 tgcttgctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATAc                                                                                          <  8:66056/149‑1 (MQ=255)
                                                 tgcttgctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATAc                                                                                          <  1:67652/149‑1 (MQ=255)
                                                 tgcttgctCCAACTGCTGCGGTAGGCGGCGCCGCTGACGGCGGCCGGCTGACCGTGGCTGTCCTTCAGAACATCATGTGCCTTGGCATTACTGTAATACTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATAc                                                                                          <  7:47494/149‑1 (MQ=255)
                                                   cttgctCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATAcc                                                                                         <  8:254078/148‑1 (MQ=255)
                                                        tCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTc                                                                                    >  7:293830/1‑148 (MQ=255)
                                                        tCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTc                                                                                    >  2:141713/1‑148 (MQ=255)
                                                                ctgcGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTTGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGc                                                                             <  5:161033/147‑1 (MQ=255)
                                                                ctgcGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCAt                                                                           >  7:101877/1‑149 (MQ=255)
                                                                   cGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTAc                                                                        <  1:112387/149‑1 (MQ=255)
                                                                                      gggCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGtgccgctg                                                     >  2:42814/1‑149 (MQ=255)
                                                                                       ggcggcCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTAAAGCCTTGGATACGTGGGGTCCATACAAGTTCGAACGGCATTACAGCACACTGGGtgccgctgc                                                    >  8:194726/1‑149 (MQ=255)
                                                                                        gcggcCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGtgccgctgcc                                                   <  6:84052/149‑1 (MQ=255)
                                                                                             cggcTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGa                                              <  8:293830/149‑1 (MQ=255)
                                                                                                   aCCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGTAACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGCGTGCCCCTGCCGCTGAGCTTg                                         >  3:132006/1‑148 (MQ=255)
                                                                                                   aCCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCtt                                          <  1:42814/147‑1 (MQ=255)
                                                                                                            gTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTgg                                                              <  7:121251/118‑1 (MQ=255)
                                                                                                            gTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCAgg                                                              >  8:121251/1‑118 (MQ=255)
                                                                                                             tCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCGCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGcc                              <  1:159162/149‑1 (MQ=255)
                                                                                                                ttGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGa                           >  3:250254/1‑149 (MQ=255)
                                                                                                                 tGAGAACATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGAt                          >  5:110229/1‑149 (MQ=255)
                                                                                                                     aaCATCTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTc                        >  4:127301/1‑147 (MQ=255)
                                                                                                                          cTTGTGCCTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTg                 <  6:314027/149‑1 (MQ=255)
                                                                                                                                ccTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGCCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAggg           >  3:53565/1‑149 (MQ=255)
                                                                                                                                 cTTGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGt          <  1:141713/149‑1 (MQ=255)
                                                                                                                                  ttGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGaaa                                      <  7:225897/120‑1 (MQ=255)
                                                                                                                                  ttGGCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGaaa                                      >  8:225897/1‑120 (MQ=255)
                                                                                                                                    ggCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGtt         <  8:101877/147‑1 (MQ=255)
                                                                                                                                    ggCATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTc        <  3:310878/148‑1 (MQ=255)
                                                                                                                                      cATTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGc     >  6:270045/1‑149 (MQ=255)
                                                                                                                                       aTTACTGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCt    >  3:287539/1‑149 (MQ=255)
                                                                                                                                          aCTGTAATAGTAGCCGGCACGATATTCCCCTTCCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCg  >  6:165785/1‑148 (MQ=255)
                                                                                                                                            tGTAATAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCg  >  5:187011/1‑146 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GTCGGACGCCAGCGAGGTGACCTGCTGCTGGGCGCCAATCCACAAGCCGTGCTTGCTCGAACTGCTGCGGTAGGCGGCGCCGCTGAGGGCGGCCGGCTGACCGTTGCTGTCCTTGAGAACATCTTGTGCCTTGGCATTACTGTAGTAGTAGCCGGCACGATATTCCCCTTTCAAGCCTTGGATACGTGGGGTCCATACCAGTTCGAACGGCATTACCGCACCCTGGGTGCCGCTGCCGCTGAGCTTGAAACCATTGCCGGATTCGAGGTTGGAAGGGTTCTGCTCG  >  NC_002947/1647783‑1648068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑