Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,670,628 C→A 8.5% intergenic (+51/‑44) rplS → / → PP_1466 50S ribosomal protein L19/acyl‑CoA thioesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,670,6280CA 8.5% 131.5 / 2.2 44intergenic (+51/‑44)rplS/PP_146650S ribosomal protein L19/acyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base A (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

GTGAAACGTCGTGGTGACGTGCGTAAAGCCAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAGGCGGTGTTCCCCAATACCACCAACCACCACAACACCCTG  >  NC_002947/1670485‑1670773
                                                                                                                                               |                                                                                                                                                 
gTGAAACGTCGTGGTGACGTGCGTAAAGCCAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCATCCTTCGGgct                                                                                                                                              >  6:284644/1‑149 (MQ=255)
     aCGTCGTGGTGACGTGCGTAAAGCCAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCCGAAGGCTGCttt                                                                                                                                         <  6:247611/149‑1 (MQ=255)
        tcgtGGTGACGTGCGTAAAGCCAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCtttttt                                                                                                                                      <  8:171506/149‑1 (MQ=255)
          gtggtgACGTGCGTAAAGCCAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGc                                                                                                                                    >  4:220340/1‑149 (MQ=255)
           tggtgACGTGCGTAAAGCCAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCCGAAGGCTGCttttttctc                                                                                                                                   <  6:172330/149‑4 (MQ=255)
                            ccAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGgctg                                                                                                                                             >  1:312612/1‑122 (MQ=255)
                            ccAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGgctg                                                                                                                                             <  2:312612/122‑1 (MQ=255)
                             cAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAAt                                                                                                                 <  2:264104/149‑1 (MQ=255)
                                    tactacCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGa                                                                                                             <  5:95084/146‑1 (MQ=255)
                                    tactacCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGAtt                                                                                                           <  3:220340/148‑1 (MQ=255)
                                        acCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCAt                                                                                                      >  1:73513/1‑149 (MQ=255)
                                         ccTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGa                                                                                                             <  6:145607/141‑1 (MQ=255)
                                         ccTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGa                                                                                                             >  5:145607/1‑141 (MQ=255)
                                         ccTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCAt                                                                                                      >  5:259615/1‑148 (MQ=255)
                                          cTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCAt                                                                                                      >  7:241376/1‑147 (MQ=255)
                                                 aCCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCg                                                                                             <  6:259615/149‑1 (MQ=255)
                                                     gTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGAc                                                                                         >  2:323866/1‑149 (MQ=255)
                                                      tCCGGCAACGCCGCTCGCATCAAGGAAAACCTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCTAGAAAAAAGGAGCCCTCGGGCTGCTTTTTTGCGTTTTGAAACCCCG‑AAATGTGATTGCCATGACGACCCGAGAc                                                                                         <  2:87757/147‑1 (MQ=255)
                                                      tCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCgaga                                                                                          <  4:168598/147‑1 (MQ=255)
                                                          gCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAgg                                                                                     >  2:96258/1‑148 (MQ=255)
                                                           cAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAg                                                                                      <  4:193513/146‑1 (MQ=255)
                                                           cAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAg                                                                                      >  3:193513/1‑146 (MQ=255)
                                                                ccGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCa                                                                              <  1:323866/149‑1 (MQ=255)
                                                                         tCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCAc                                                                     >  5:301353/1‑149 (MQ=255)
                                                                         tCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCAc                                                                     >  5:35173/1‑149 (MQ=255)
                                                                         tCAAGGAAAAACTGTCCTGAGTGCAGTTAGCCCGGTGGCCGAGGCAGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTCAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACAAGGAAATCCAGCGCCGCAc                                                                     >  3:296180/1‑149 (MQ=255)
                                                                             ggtaaaaCTTTCCTGAGTGCAGTTTGCCCGGTGGACGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTTCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGaa                                                                 <  4:296180/146‑1 (MQ=255)
                                                                                  aaCTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTAGGGATGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGAGCAGCACCGAACTGTc                                                            >  3:75720/1‑149 (MQ=255)
                                                                                         cTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGAcc                                                     >  3:238850/1‑149 (MQ=255)
                                                                                             gTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCcgcg                                                 <  1:96258/149‑1 (MQ=255)
                                                                                             gTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTATGAACCCCCGAAAATCTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGGGACCcgcg                                                 <  5:284644/149‑1 (MQ=255)
                                                                                                  gTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTAcc                                            <  2:73513/149‑1 (MQ=255)
                                                                                                   tttGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTAcc                                            >  3:67238/1‑148 (MQ=255)
                                                                                                   tttGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTAcc                                            >  3:241866/1‑148 (MQ=255)
                                                                                                      gCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAg                                         <  8:241376/148‑1 (MQ=255)
                                                                                                        ccGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAgg                                        <  1:122155/147‑1 (MQ=255)
                                                                                                         cGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAgg                                        >  2:122155/1‑146 (MQ=255)
                                                                                                            tGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCcgc                                                  >  6:8075/1‑133 (MQ=255)
                                                                                                            tGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCcgc                                                  <  5:8075/133‑1 (MQ=255)
                                                                                                                    gccgccTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAGGCGGTGTTCCCCAAt                          <  4:241866/149‑1 (MQ=255)
                                                                                                                     ccgccTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAGGCGGTGTTCCCCAATa                         <  6:83028/149‑1 (MQ=255)
                                                                                                                            aacgcaaaaaaGCAGCCCGAAGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAGGCGGTGTTCCCCAATaccaccaa                  >  2:205494/6‑149 (MQ=255)
                                                                                                                                    aaaGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAGGCGGTGTTCCCCAATACCACCAACcaccac           >  2:86781/1‑148 (MQ=255)
                                                                                                                                            cTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAGGCGGTGTTCCCCAATACCACCAACCACCACAACACCCTg  >  8:185891/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GTGAAACGTCGTGGTGACGTGCGTAAAGCCAAGCTGTACTACCTGCGCGACCTGTCCGGCAAAGCCGCTCGCATCAAGGAAAAACTGTCCTGAGTGCAGTTTGCCCGGTGGCCGAGGCCGCCTAGCGAGAAAAAAGCAGCCTTCGGGCTGCTTTTTTGCGTTTTGAAACCCCGAAAATGTGATTGCCATGACGACCCGAGACCAGGAAATCCAGCGCCGCACCGAACTGTCGGTGACCCGCGTTACCAAGGCGGTGTTCCCCAATACCACCAACCACCACAACACCCTG  >  NC_002947/1670485‑1670773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑