Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,679,058	C→T	5.3%	A175T (GCG→ACG)	PP_1474 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,679,058	0	C	T	5.3%	228.1 / 2.0	75	A175T (GCG→ACG)	PP_1474	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/36);  new base T (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

TGAAACTGGTAGTCAGCCAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGTGCCACCAGCGCCTG  >  NC_002947/1678910‑1679202                                                                                                                                                       |                                                                                                                                                   tGAAACTGGTAGTCAGCCAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAgcgc                                                                                                                                                    >  3:225303/1‑149 (MQ=255)  gAAACTGGTAGTCAGCCAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAgcgcg                                                                                                                                                   >  6:295735/1‑149 (MQ=255)     aCTGGTAGTCAGCCAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTg                                                                                                                                                <  5:295735/149‑1 (MQ=255)       tGGTAGTCAGCCAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GCTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑ATGAGCGCGCTGTc                                                                                                                                              >  1:22501/1‑149 (MQ=255)          tAGTCAGCCAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCggg                                                                                                                                           >  1:314273/1‑149 (MQ=255)                 ccAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTgg                                                                                                                                    <  3:6432/149‑1 (MQ=255)                  cAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCGGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGgc                                                                                                                                   <  3:232724/149‑1 (MQ=255)                   aGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGgcg                                                                                                                                  >  3:121925/1‑149 (MQ=255)                   aGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGgcg                                                                                                                                  <  4:121925/149‑1 (MQ=255)                    gTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGAGGCGG‑CTGAGCGTGCTGTCGGGCAACTGgcg                                                                                                                                  >  3:95402/1‑148 (MQ=255)                    gTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGACGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGgcgc                                                                                                                                 <  6:288397/149‑1 (MQ=255)                        cccGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGa                                                                                                                             >  8:297311/1‑149 (MQ=255)                            ccTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCt                                                                                                                         <  3:126032/149‑1 (MQ=255)                                       gATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGc                                                                                                               <  5:151913/148‑1 (MQ=255)                                       gATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCg                                                                                                              >  3:281485/1‑149 (MQ=255)                                           aGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑c                                                                                                          <  4:281485/148‑1 (MQ=255)                                             gTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCg                                                                                                        >  3:148508/1‑148 (MQ=255)                                             gTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCCGCGTG‑CCg                                                                                                        >  2:64037/1‑148 (MQ=255)                                              tAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCg                                                                                                        >  7:156669/1‑147 (MQ=255)                                                    aCTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTgcg                                                                                                >  5:2304/1‑149 (MQ=255)                                                      ttCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACAGGGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTgcgc                                                                                               >  1:93920/1‑149 (MQ=255)                                                      ttCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCt                                                                                              >  4:119204/1‑149 (MQ=255)                                                         aGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAAt                                                                                                                   <  6:93808/126‑1 (MQ=255)                                                         aGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCgg                                                                                           >  7:283934/1‑149 (MQ=255)                                                          ggCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCATTTGCTGCATCTGCAACA‑GGTTGGCTGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGc                                                                                          <  4:77847/149‑1 (MQ=255)                                                           gCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGc                                                                                          >  2:170252/1‑148 (MQ=255)                                                            ccAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGa                                                                                        <  6:276404/149‑1 (MQ=255)                                                             cAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAAAA‑GATTGGCGGAGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGa                                                                                        >  7:209860/1‑148 (MQ=255)                                                              aGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGAc                                                                                       >  4:271511/1‑148 (MQ=255)                                                               gTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTTGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACaa                                                                                     >  7:42633/1‑149 (MQ=255)                                                                tAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTg                                                                                                            >  2:297669/1‑126 (MQ=255)                                                                tAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTg                                                                                                            <  1:297669/126‑1 (MQ=255)                                                                tAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAAt                                                                                    >  3:153087/1‑149 (MQ=255)                                                                      aGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAg                                                                              <  5:317317/149‑1 (MQ=255)                                                                         aCCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCtt                                                                           >  1:195076/1‑149 (MQ=255)                                                                          ccAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCttt                                                                          <  2:195076/149‑1 (MQ=255)                                                                          ccAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCttt                                                                          <  4:274499/149‑1 (MQ=255)                                                                          ccAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCtt                                                                           >  8:169330/1‑148 (MQ=255)                                                                           caggcgcaggcgCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCtt                                                                           >  2:89420/1‑147 (MQ=255)                                                                            aggcgcaggcgcCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAg                                                                        <  2:314273/149‑1 (MQ=255)                                                                              gcgcaggcgcCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCg                                                                      >  1:258124/1‑149 (MQ=255)                                                                              gcgcaggcgcCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCg                                                                      <  8:283934/149‑1 (MQ=255)                                                                                   ggcgcCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGTGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGcc                                                                 <  4:95402/149‑1 (MQ=255)                                                                                      gcCCCAGTTGCTGCATGTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGTCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCTGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTg                                                              <  8:209860/149‑1 (MQ=255)                                                                                        cccAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCt                                                            >  2:217092/1‑149 (MQ=255)                                                                                         ccAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGGACTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCt                                                            >  1:259284/1‑149 (MQ=255)                                                                                         ccAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTc                                                           <  2:258124/149‑1 (MQ=255)                                                                                         ccAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CAGAGCGCGCTGTCGAGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCt                                                            >  5:236347/1‑148 (MQ=255)                                                                                            gTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGTGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTcag                                                         >  5:84385/1‑148 (MQ=255)                                                                                                ctgcATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAg‑g                                                    <  4:148508/148‑1 (MQ=255)                                                                                                ctgcATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑Gc                                                   <  5:5661/149‑1 (MQ=255)                                                                                                ctgcATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCGGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAg‑g                                                    <  7:297311/148‑1 (MQ=255)                                                                                                 tgcATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑Gc                                                   >  3:270773/1‑148 (MQ=255)                                                                                                   cATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTGCCCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑Gc                                                   <  1:89420/147‑1 (MQ=255)                                                                                                   cATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑Gcc                                                  <  2:259284/147‑1 (MQ=255)                                                                                                        gCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAgg                                           >  8:253125/1‑149 (MQ=255)                                                                                                        gCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAgg                                           >  8:292242/1‑149 (MQ=255)                                                                                                        gCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAgg                                           >  2:210146/1‑149 (MQ=255)                                                                                                         cAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAgg                                           <  7:253125/148‑1 (MQ=255)                                                                                                         cAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAg                                            <  3:119204/147‑1 (MQ=255)                                                                                                          aaCA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTc                                         <  8:156669/149‑1 (MQ=255)                                                                                                           aCA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCtt                                                               >  8:118891/1‑127 (MQ=255)                                                                                                           aCA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCtt                                                               <  7:118891/127‑1 (MQ=255)                                                                                                            cA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCa                                       <  4:225303/149‑1 (MQ=255)                                                                                                             a‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCAc                                      <  4:270773/149‑1 (MQ=255)                                                                                                             a‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCAc                                      <  6:2304/149‑1 (MQ=255)                                                                                                                               gTTGCCGTAGTGGCGG‑CTGAGCGTGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAg                       <  6:84385/147‑1 (MQ=255)                                                                                                                               gTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGt                     <  2:22501/149‑1 (MQ=255)                                                                                                                               gTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCCGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAgg                      <  1:64037/148‑1 (MQ=255)                                                                                                                                    cGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCCTGCAGGTCCACCTGATGGCCGTCGAGGTAGGGt                <  1:217092/149‑1 (MQ=255)                                                                                                                                    cGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGt                >  1:161463/1‑149 (MQ=255)                                                                                                                                         tGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAGCAGAATGATGGTCCACATGATGGCCGTCGAGGTAGGGTGcaa            >  1:283691/1‑147 (MQ=255)                                                                                                                                           gcgg‑cTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGTGccac           >  5:129933/1‑147 (MQ=255)                                                                                                                                             gg‑cTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGTGCCACCAgc       >  7:101159/1‑149 (MQ=255)                                                                                                                                                cTGAGCGCGCTGTAGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGTGCCACCAgcgc     >  7:45567/1‑149 (MQ=255)                                                                                                                                                   aGCGCGCTGTTGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGTGCCACCAGCGCCTg  <  8:42633/149‑1 (MQ=255)                                                                                                                                                   aGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGTGCCACCAGCGCCTg  <  7:169330/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   TGAAACTGGTAGTCAGCCAGTGCCCCGCCTTGTTGTTGGATCAGGTAGCGCACTTCAGGCCAGTAGCCGAGCACCAGGCGCAGGCGCCCCAGTTGCTGCATCTGCAACA‑GGTTGGCGGTGGCCTCGTTGCCGTAGTGGCGG‑CTGAGCGCGCTGTCGGGCAACTGGCGCAGGATGCTGTCAATCTGCGTG‑CCGTAGCTGCGCTCGGCGACAATCCCCAGCTTTAGCGGGGCCTTGCTCAGCAG‑GCCATGCAGGTCCACCTGATGGCCGTCGAGGTAGGGTGCCACCAGCGCCTG  >  NC_002947/1678910‑1679202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑