| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,707,227 | G→C | 20.3% | intergenic (+45/+97) | PP_1501 → / ← PP_1502 | hypothetical protein/OmpA family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,707,227 | 0 | G | C | 20.3% | 73.6 / 12.5 | 34 | intergenic (+45/+97) | PP_1501/PP_1502 | hypothetical protein/OmpA family protein |
| Reads supporting (aligned to +/- strand): ref base G (13/14); new base C (3/4); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
TTCGACACCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTT > NC_002947/1707084‑1707368 | ttCGACACCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCgcgggc > 1:316416/1‑149 (MQ=255) ccAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCg < 2:123885/149‑1 (MQ=255) ccAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCACAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCCCGCGCGGCGCt < 4:211062/147‑1 (MQ=255) cagcagAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGa > 8:45304/1‑149 (MQ=255) cagcagAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGGGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCACCGCGCGGGCGGCGCTCGa < 7:241670/149‑1 (MQ=255) aaaGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtctcgc > 8:293656/1‑147 (MQ=255) aaaGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcac < 8:158532/149‑1 (MQ=255) aaaGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtctcgc < 3:20504/149‑3 (MQ=255) aGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtctcgcgg < 7:293656/149‑5 (MQ=255) gACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGca > 8:154320/1‑149 (MQ=255) ggCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCGCGGGCGCCaaaaa < 2:30852/149‑2 (MQ=25) gtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGc < 1:138341/149‑1 (MQ=255) gtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGc < 2:316416/149‑1 (MQ=255) gtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGACCGCCGCCCGGGCGGCGCTCGATCTCACACGCGGCACAACATTATCGGc > 2:339360/1‑149 (MQ=255) gCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCt > 2:65570/1‑149 (MQ=255) gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCTTTTct < 5:108619/149‑2 (MQ=39) gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTcc < 3:249832/149‑1 (MQ=255) aaaCACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCt > 1:134026/1‑149 (MQ=255) acacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCttt < 4:45876/149‑1 (MQ=255) acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACAtt < 5:323558/99‑1 (MQ=255) acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACAtt > 6:323558/1‑99 (MQ=255) acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGc > 7:99194/1‑105 (MQ=255) acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGc < 8:99194/105‑1 (MQ=255) acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCa < 7:45304/149‑1 (MQ=255) gTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAg > 7:298003/1‑149 (MQ=255) gAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATTGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAgcg > 7:17217/1‑149 (MQ=255) agcaTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCt > 2:200185/1‑148 (MQ=255) gcaTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCa > 7:196694/1‑149 (MQ=255) gcgcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtg > 4:135348/1‑149 (MQ=255) gcCCGCGAGAGCGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggt < 7:296359/149‑1 (MQ=255) gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCgg > 5:97750/1‑149 (MQ=255) cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACAATAAGTGGTGCCACCgcgtg > 6:234365/1‑145 (MQ=38) gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTgg < 8:298003/149‑1 (MQ=255) aGCGCCGCGCCGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCC‑TCCATAGCGGGGCTTGTCCTTTCAGCTAGACAGCCTTACCGCTCAACCTGGCACACCGAGCGTGGTGCCACCGGCTgct > 2:113989/1‑147 (MQ=255) gcgcCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGtt < 7:154320/149‑1 (MQ=255) | TTCGACACCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTT > NC_002947/1707084‑1707368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |