Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,818,484 A→G 9.2% Y209C (TAC→TGC)  PP_1622 → M23B subfamily metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,818,4840AG 9.2% 148.2 / 3.9 54Y209C (TAC→TGC) PP_1622M23B subfamily metallopeptidase
Reads supporting (aligned to +/- strand):  ref base A (26/23);  new base G (3/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTGAATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGT  >  NC_002947/1818337‑1818630
                                                                                                                                                   |                                                                                                                                                  
gTTTTAATAAAGGATTTGATTTCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGGGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTAc                                                                                                                                                   <  8:215630/149‑1 (MQ=255)
gTTTGAATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTAc                                                                                                                                                   <  3:261068/149‑1 (MQ=255)
     aaTAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGtta                                                                                                                                              >  7:143748/1‑147 (MQ=255)
      aTAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGGGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCAt                                                                                                                                             <  3:299717/149‑1 (MQ=255)
       tAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATa                                                                                                                                            <  2:144439/149‑1 (MQ=255)
                   tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGcac                                                                                                                                 <  8:143748/148‑3 (MQ=255)
                   tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGt                                                                                                                                >  1:311503/1‑149 (MQ=255)
                                    gACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGgcag                                                                                                               >  5:258473/1‑149 (MQ=255)
                                         ccTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGt                                                                                                          >  7:130504/1‑149 (MQ=255)
                                          cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGt                                                                                                          >  4:155916/1‑148 (MQ=255)
                                             tttttGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAg                                                                                                      <  7:30405/149‑1 (MQ=255)
                                                    tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcaggg                                                                                                >  8:295188/1‑148 (MQ=255)
                                                    tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcaggg                                                                                                >  3:274075/1‑148 (MQ=255)
                                                           gATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACATCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGAt                                                                                        >  5:131865/1‑149 (MQ=255)
                                                               gTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCt                                                                                    <  3:155916/149‑1 (MQ=255)
                                                                   ggtggtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGaaa                                                                                <  7:158082/149‑1 (MQ=255)
                                                                     tggtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGaaa                                                                                <  7:295188/147‑1 (MQ=255)
                                                                       gtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATggg                                                                            <  4:274075/149‑1 (MQ=255)
                                                                         ttATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                          >  4:195681/1‑149 (MQ=255)
                                                                           aTGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTc                                                                         <  2:311503/148‑1 (MQ=255)
                                                                            tGCCGGGTGTGGTTTGAGGGGGTACGGCGTGCTGATCTTCATCATGGAGAGCGACACCTTCGTCAGTGCCTACGGTCTTAACCGCAGGGTGTTGGTTCGGGAGGGGCAGCAGGTGAAGGGAGGGCAGTCGATTGCTGAAATggg                                                                            <  3:162425/144‑1 (MQ=255)
                                                                            tGCCGGGAGAGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTCTAGGTTCGGGAGGGCCAGCAGGTCAAGGCACGGCAGACGATTGCTGAAATggggagaa                                                                       >  4:162425/1‑145 (MQ=255)
                                                                              ccGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                     >  6:126253/1‑149 (MQ=255)
                                                                                     tGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGACTACGGTCATAACCGCAGGCTGTTGGTTAGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGca                                                                 >  3:66594/1‑146 (MQ=255)
                                                                                       gTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGca                                                                 >  5:9951/1‑144 (MQ=255)
                                                                                        tttGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACAGGca                                                                 >  5:323081/1‑143 (MQ=255)
                                                                                              gtgCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                          <  6:171564/126‑1 (MQ=255)
                                                                                              gggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCATGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                          >  5:171564/1‑128 (MQ=255)
                                                                                                    cGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCTGGATGTGCAGCAGGTCAAGGCAGGGCAGTCGAATGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                               <  2:139661/149‑1 (MQ=255)
                                                                                                       cGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCAtt                                            <  6:9951/149‑1 (MQ=255)
                                                                                                          gCTTATAATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGATCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCg                                         <  6:131865/149‑1 (MQ=255)
                                                                                                                catcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                       >  5:263674/1‑129 (MQ=255)
                                                                                                                catcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                       <  6:263674/129‑1 (MQ=255)
                                                                                                                 atcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCaa                                                                                                       >  8:215980/1‑80 (MQ=255)
                                                                                                                   catcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGTGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgcc                                >  6:343727/1‑149 (MQ=255)
                                                                                                                     tcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTctccgc                              >  7:147806/1‑149 (MQ=255)
                                                                                                                        aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                               >  3:294551/1‑129 (MQ=255)
                                                                                                                        aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                               <  4:294551/129‑1 (MQ=255)
                                                                                                                        aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAg                           >  2:127078/1‑149 (MQ=255)
                                                                                                                             aGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCa                       >  2:260088/1‑148 (MQ=255)
                                                                                                                              gCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCaaa                     <  1:260088/149‑1 (MQ=255)
                                                                                                                                   acCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTg                                                <  7:216375/117‑1 (MQ=255)
                                                                                                                                   acCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTg                                                >  8:216375/1‑117 (MQ=255)
                                                                                                                                   acCTACGTCAGTGCCTACGGTCAACACCCCAGGCCGTTGGGTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGGCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                <  4:66594/149‑1 (MQ=255)
                                                                                                                                     cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTc               >  2:233418/1‑148 (MQ=255)
                                                                                                                                        cGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACAGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGa             <  6:323081/147‑1 (MQ=255)
                                                                                                                                         gTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATc           >  4:308686/1‑148 (MQ=255)
                                                                                                                                            aGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACt       <  1:127078/149‑1 (MQ=255)
                                                                                                                                              tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTcc     <  8:130504/149‑1 (MQ=255)
                                                                                                                                              agCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGCGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTc      >  6:11687/2‑148 (MQ=255)
                                                                                                                                                ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAggg                         >  7:88172/1‑127 (MQ=255)
                                                                                                                                                ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAggg                         <  8:88172/127‑1 (MQ=255)
                                                                                                                                                 cTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt  >  7:88618/1‑149 (MQ=255)
                                                                                                                                                 cTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt  >  4:262467/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTTGAATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGT  >  NC_002947/1818337‑1818630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑