Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,818,489 C→T 7.2% H211Y (CAT→TAT)  PP_1622 → M23B subfamily metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,818,4890CT 7.2% 158.9 / 2.0 55H211Y (CAT→TAT) PP_1622M23B subfamily metallopeptidase
Reads supporting (aligned to +/- strand):  ref base C (28/23);  new base T (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

ATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCT  >  NC_002947/1818343‑1818634
                                                                                                                                                  |                                                                                                                                                 
aTAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGGGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCAt                                                                                                                                                 <  3:299717/149‑1 (MQ=255)
 tAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATa                                                                                                                                                <  2:144439/149‑1 (MQ=255)
             tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGcac                                                                                                                                     <  8:143748/148‑3 (MQ=255)
             tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGt                                                                                                                                    >  1:311503/1‑149 (MQ=255)
                              gACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGgcag                                                                                                                   >  5:258473/1‑149 (MQ=255)
                                   ccTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGt                                                                                                              >  7:130504/1‑149 (MQ=255)
                                    cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGt                                                                                                              >  4:155916/1‑148 (MQ=255)
                                       tttttGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAg                                                                                                          <  7:30405/149‑1 (MQ=255)
                                              tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcaggg                                                                                                    >  8:295188/1‑148 (MQ=255)
                                              tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcaggg                                                                                                    >  3:274075/1‑148 (MQ=255)
                                                     gATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACATCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGAt                                                                                            >  5:131865/1‑149 (MQ=255)
                                                         gTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCt                                                                                        <  3:155916/149‑1 (MQ=255)
                                                             ggtggtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGaaa                                                                                    <  7:158082/149‑1 (MQ=255)
                                                               tggtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGaaa                                                                                    <  7:295188/147‑1 (MQ=255)
                                                                 gtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATggg                                                                                <  4:274075/149‑1 (MQ=255)
                                                                   ttATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                              >  4:195681/1‑149 (MQ=255)
                                                                     aTGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTc                                                                             <  2:311503/148‑1 (MQ=255)
                                                                      tGCCGGGTGTGGTTTGAGGGGGTACGGCGTGCTGATCTTCATCATGGAGAGCGACACCTTCGTCAGTGCCTACGGTCTTAACCGCAGGGTGTTGGTTCGGGAGGGGCAGCAGGTGAAGGGAGGGCAGTCGATTGCTGAAATggg                                                                                <  3:162425/144‑1 (MQ=255)
                                                                      tGCCGGGAGAGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTCTAGGTTCGGGAGGGCCAGCAGGTCAAGGCACGGCAGACGATTGCTGAAATggggagaa                                                                           >  4:162425/1‑145 (MQ=255)
                                                                        ccGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                         >  6:126253/1‑149 (MQ=255)
                                                                               tGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGACTACGGTCATAACCGCAGGCTGTTGGTTAGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGca                                                                     >  3:66594/1‑146 (MQ=255)
                                                                                 gTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGca                                                                     >  5:9951/1‑144 (MQ=255)
                                                                                  tttGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACAGGca                                                                     >  5:323081/1‑143 (MQ=255)
                                                                                        gtgCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                              <  6:171564/126‑1 (MQ=255)
                                                                                        gggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCATGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                              >  5:171564/1‑128 (MQ=255)
                                                                                              cGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCTGGATGTGCAGCAGGTCAAGGCAGGGCAGTCGAATGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                                   <  2:139661/149‑1 (MQ=255)
                                                                                                 cGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCAtt                                                <  6:9951/149‑1 (MQ=255)
                                                                                                    gCTTATAATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGATCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCg                                             <  6:131865/149‑1 (MQ=255)
                                                                                                          catcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                           <  6:263674/129‑1 (MQ=255)
                                                                                                          catcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                           >  5:263674/1‑129 (MQ=255)
                                                                                                           atcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCaa                                                                                                           >  8:215980/1‑80 (MQ=255)
                                                                                                             catcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGTGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgcc                                    >  6:343727/1‑149 (MQ=255)
                                                                                                               tcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTctccgc                                  >  7:147806/1‑149 (MQ=255)
                                                                                                                  aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                                   <  4:294551/129‑1 (MQ=255)
                                                                                                                  aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                                   >  3:294551/1‑129 (MQ=255)
                                                                                                                  aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAg                               >  2:127078/1‑149 (MQ=255)
                                                                                                                       aGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCa                           >  2:260088/1‑148 (MQ=255)
                                                                                                                        gCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCaaa                         <  1:260088/149‑1 (MQ=255)
                                                                                                                             acCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTg                                                    <  7:216375/117‑1 (MQ=255)
                                                                                                                             acCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTg                                                    >  8:216375/1‑117 (MQ=255)
                                                                                                                             acCTACGTCAGTGCCTACGGTCAACACCCCAGGCCGTTGGGTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGGCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                    <  4:66594/149‑1 (MQ=255)
                                                                                                                               cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTc                   >  2:233418/1‑148 (MQ=255)
                                                                                                                                  cGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACAGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGa                 <  6:323081/147‑1 (MQ=255)
                                                                                                                                   gTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATc               >  4:308686/1‑148 (MQ=255)
                                                                                                                                      aGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACt           <  1:127078/149‑1 (MQ=255)
                                                                                                                                        tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTcc         <  8:130504/149‑1 (MQ=255)
                                                                                                                                        agCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGCGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTc          >  6:11687/2‑148 (MQ=255)
                                                                                                                                          ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAggg                             <  8:88172/127‑1 (MQ=255)
                                                                                                                                          ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAggg                             >  7:88172/1‑127 (MQ=255)
                                                                                                                                           cTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt      >  7:88618/1‑149 (MQ=255)
                                                                                                                                           cTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt      >  4:262467/1‑149 (MQ=255)
                                                                                                                                              cGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGtt     <  6:258473/147‑1 (MQ=255)
                                                                                                                                               ggTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCt  >  4:290000/1‑149 (MQ=255)
                                                                                                                                                gTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAgg                              <  5:247295/120‑1 (MQ=255)
                                                                                                                                                gTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAgg                              >  6:247295/1‑120 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCT  >  NC_002947/1818343‑1818634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑