Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,818,492 A→G 7.6% N212D (AAC→GAC)  PP_1622 → M23B subfamily metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,818,4920AG 7.6% 146.5 / 2.7 53N212D (AAC→GAC) PP_1622M23B subfamily metallopeptidase
Reads supporting (aligned to +/- strand):  ref base A (28/20);  new base G (2/2);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGC  >  NC_002947/1818344‑1818636
                                                                                                                                                    |                                                                                                                                                
tAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATa                                                                                                                                                  <  2:144439/149‑1 (MQ=255)
            tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGcac                                                                                                                                       <  8:143748/148‑3 (MQ=255)
            tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGt                                                                                                                                      >  1:311503/1‑149 (MQ=255)
                             gACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGgcag                                                                                                                     >  5:258473/1‑149 (MQ=255)
                                  ccTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGt                                                                                                                >  7:130504/1‑149 (MQ=255)
                                   cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGt                                                                                                                >  4:155916/1‑148 (MQ=255)
                                      tttttGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAg                                                                                                            <  7:30405/149‑1 (MQ=255)
                                             tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcaggg                                                                                                      >  8:295188/1‑148 (MQ=255)
                                             tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcaggg                                                                                                      >  3:274075/1‑148 (MQ=255)
                                                    gATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACATCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGAt                                                                                              >  5:131865/1‑149 (MQ=255)
                                                        gTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCt                                                                                          <  3:155916/149‑1 (MQ=255)
                                                            ggtggtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGaaa                                                                                      <  7:158082/149‑1 (MQ=255)
                                                              tggtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGaaa                                                                                      <  7:295188/147‑1 (MQ=255)
                                                                gtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATggg                                                                                  <  4:274075/149‑1 (MQ=255)
                                                                  ttATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                                >  4:195681/1‑149 (MQ=255)
                                                                    aTGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTc                                                                               <  2:311503/148‑1 (MQ=255)
                                                                     tGCCGGGTGTGGTTTGAGGGGGTACGGCGTGCTGATCTTCATCATGGAGAGCGACACCTTCGTCAGTGCCTACGGTCTTAACCGCAGGGTGTTGGTTCGGGAGGGGCAGCAGGTGAAGGGAGGGCAGTCGATTGCTGAAATggg                                                                                  <  3:162425/144‑1 (MQ=255)
                                                                     tGCCGGGAGAGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTCTAGGTTCGGGAGGGCCAGCAGGTCAAGGCACGGCAGACGATTGCTGAAATggggagaa                                                                             >  4:162425/1‑145 (MQ=255)
                                                                       ccGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                           >  6:126253/1‑149 (MQ=255)
                                                                              tGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGACTACGGTCATAACCGCAGGCTGTTGGTTAGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGca                                                                       >  3:66594/1‑146 (MQ=255)
                                                                                gTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGca                                                                       >  5:9951/1‑144 (MQ=255)
                                                                                 tttGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACAGGca                                                                       >  5:323081/1‑143 (MQ=255)
                                                                                       gtgCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                                <  6:171564/126‑1 (MQ=255)
                                                                                       gggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCATGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                                >  5:171564/1‑128 (MQ=255)
                                                                                             cGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCTGGATGTGCAGCAGGTCAAGGCAGGGCAGTCGAATGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                                     <  2:139661/149‑1 (MQ=255)
                                                                                                cGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCAtt                                                  <  6:9951/149‑1 (MQ=255)
                                                                                                   gCTTATAATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGATCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCg                                               <  6:131865/149‑1 (MQ=255)
                                                                                                         catcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                             <  6:263674/129‑1 (MQ=255)
                                                                                                         catcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                             >  5:263674/1‑129 (MQ=255)
                                                                                                          atcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCaa                                                                                                             >  8:215980/1‑80 (MQ=255)
                                                                                                            catcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGTGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgcc                                      >  6:343727/1‑149 (MQ=255)
                                                                                                              tcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTctccgc                                    >  7:147806/1‑149 (MQ=255)
                                                                                                                 aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                                     <  4:294551/129‑1 (MQ=255)
                                                                                                                 aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGc                                                     >  3:294551/1‑129 (MQ=255)
                                                                                                                 aGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAg                                 >  2:127078/1‑149 (MQ=255)
                                                                                                                      aGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCa                             >  2:260088/1‑148 (MQ=255)
                                                                                                                       gCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCaaa                           <  1:260088/149‑1 (MQ=255)
                                                                                                                            acCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTg                                                      >  8:216375/1‑117 (MQ=255)
                                                                                                                            acCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTg                                                      <  7:216375/117‑1 (MQ=255)
                                                                                                                            acCTACGTCAGTGCCTACGGTCAACACCCCAGGCCGTTGGGTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGGCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                      <  4:66594/149‑1 (MQ=255)
                                                                                                                              cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTc                     >  2:233418/1‑148 (MQ=255)
                                                                                                                                 cGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACAGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGa                   <  6:323081/147‑1 (MQ=255)
                                                                                                                                  gTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATc                 >  4:308686/1‑148 (MQ=255)
                                                                                                                                     aGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACt             <  1:127078/149‑1 (MQ=255)
                                                                                                                                       tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTcc           <  8:130504/149‑1 (MQ=255)
                                                                                                                                       agCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGCGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTc            >  6:11687/2‑148 (MQ=255)
                                                                                                                                         ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAggg                               >  7:88172/1‑127 (MQ=255)
                                                                                                                                         ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAggg                               <  8:88172/127‑1 (MQ=255)
                                                                                                                                          cTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt        >  7:88618/1‑149 (MQ=255)
                                                                                                                                          cTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt        >  4:262467/1‑149 (MQ=255)
                                                                                                                                             cGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGtt       <  6:258473/147‑1 (MQ=255)
                                                                                                                                              ggTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCt    >  4:290000/1‑149 (MQ=255)
                                                                                                                                               gTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAgg                                <  5:247295/120‑1 (MQ=255)
                                                                                                                                               gTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAgg                                >  6:247295/1‑120 (MQ=255)
                                                                                                                                                 cagaaCCGCAGGCGGGTTGTTCGGGATGGGCAGCAGGTCAAGGCAGGTCTGTCTATTGCTGAAATGGGGTCTTCGGTAACAGATCGTGTGAAGCTGCATTTCTAGATTCGCCGCCAGGGCAAACACGTCGATCCACTCCAGTTCCTGc  <  8:147806/145‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGC  >  NC_002947/1818344‑1818636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑