Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,843,370	G→T	54.9%	T475N (ACC→AAC)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,843,370	0	G	T	54.9%	12.6 / 84.7	62	T475N (ACC→AAC)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base G (12/16);  new base T (18/16);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CTGCGGTGGCAGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGC  >  NC_002947/1843222‑1843518                                                                                                                                                     |                                                                                                                                                      cTGCGGTGGCAGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCgg                                                                                                                                                       >  5:185992/1‑149 (MQ=255)         gCAGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTgccgcc                                                                                                                                                <  8:131633/148‑1 (MQ=255)          cAGCGCTTCGGCATCGGCATGAGCCTTGGGCAGGTTGAGGCTGATCCACAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCgg                                                                                                                                              <  7:207295/149‑1 (MQ=255)                ttCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGc                                                                                                                                        >  7:8992/1‑149 (MQ=255)                  cGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGc                                                                                                                                        <  6:190405/147‑1 (MQ=255)                             tGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGtt                                                                                                                           <  5:43139/149‑1 (MQ=255)                                gCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGt                                                                                                                         >  5:47919/1‑148 (MQ=255)                                 ccTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGt                                                                                                                         >  7:270775/1‑147 (MQ=255)                                 ccTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCACGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGt                                                                                                                         >  7:244508/1‑147 (MQ=255)                                       gCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCCATCTCGCCGCCCATCTGTTCGATCAGGCGCTAGGAAATCACCAGCCCCAGACCGGTGCCGCCGCGCTGGCGGGCCAGTTAGTTGTCAGCCTgc                                                                                                                 >  8:162744/1‑148 (MQ=255)                                            ttGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGaa                                                                                                            <  2:167315/149‑1 (MQ=255)                                               aGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGc                                                                                                         >  6:52358/1‑149 (MQ=255)                                                  cTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTg                                                                                                      <  6:185992/149‑1 (MQ=255)                                                     aTCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGaa                                                                                                   >  5:252704/1‑149 (MQ=255)                                                     aTCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGaa                                                                                                   <  7:162744/149‑1 (MQ=255)                                                       ccAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACa                                                                                                 <  1:153077/149‑1 (MQ=255)                                                        cAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGTCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACa                                                                                                 <  1:50194/148‑1 (MQ=255)                                                        cAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACa                                                                                                 <  5:52358/148‑1 (MQ=255)                                                        cAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAg                                                                                                >  6:125096/1‑149 (MQ=255)                                                         aGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAgt                                                                                               >  2:146848/1‑149 (MQ=255)                                                         aGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAgt                                                                                               <  3:72105/149‑1 (MQ=255)                                                           aaCTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAgtgt                                                                                             <  2:176197/149‑1 (MQ=255)                                                                cGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTgcgc                                                                                         >  8:286291/1‑148 (MQ=255)                                                                   aCCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACAt                                                                                     >  2:203377/1‑149 (MQ=255)                                                                      ttCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCCGGCCACTGAGTTGTCAGCCTGGCTGAAGGCCTGGATCCGTGTGCGCACCTccg                                                                                  >  8:305203/1‑148 (MQ=255)                                                                       tCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑t                                                                                 >  4:257693/1‑148 (MQ=255)                                                                         gCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑Tg                                                                                >  3:249995/1‑147 (MQ=255)                                                                         gCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAg                                                                              <  6:47919/149‑1 (MQ=255)                                                                         gCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGa                                                                               <  8:8992/148‑1 (MQ=255)                                                                         gCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGa                                                                               <  7:286291/148‑1 (MQ=255)                                                                         gCCCGGGGTACTGTCGACACCGAGCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑Tg                                                                                <  4:249995/147‑1 (MQ=255)                                                                          cccGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAgg                                                                             <  1:146848/149‑1 (MQ=255)                                                                           ccGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGt                                                                            >  3:254316/1‑149 (MQ=255)                                                                                gTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAg                                                                       <  3:53441/149‑1 (MQ=255)                                                                                           acCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCgg                                                            >  4:198779/1‑149 (MQ=255)                                                                                             cGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGgtg                                                          >  5:16078/1‑149 (MQ=255)                                                                                              gATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTAAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGgtgt                                                         >  2:254674/1‑149 (MQ=255)                                                                                                    gccgccCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTcc                                                       <  3:257693/145‑1 (MQ=255)                                                                                                                   gATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACt                                    >  5:54652/1‑149 (MQ=255)                                                                                                                     tCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTgc                                  >  1:258807/1‑149 (MQ=255)                                                                                                                       aGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTgcgc                                <  1:134440/149‑1 (MQ=255)                                                                                                                         gcgcTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTg                                                     >  4:87434/1‑126 (MQ=255)                                                                                                                         gcgcTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTg                                                     <  3:87434/126‑1 (MQ=255)                                                                                                                         gcgcTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTgcgcg                               >  5:65878/1‑148 (MQ=255)                                                                                                                           gcTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCa                             <  1:254674/149‑1 (MQ=255)                                                                                                                           gcTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAg                            >  1:252693/1‑149 (MQ=255)                                                                                                                                gAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTc                       <  6:65878/149‑1 (MQ=255)                                                                                                                                   aTCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTg                    >  1:120544/1‑149 (MQ=255)                                                                                                                                   aTCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTg                    >  1:188779/1‑149 (MQ=255)                                                                                                                                   aTCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTTCGCGCAGTCTTCGTg                    <  8:270775/149‑1 (MQ=255)                                                                                                                                      aCCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                 >  3:124353/1‑149 (MQ=255)                                                                                                                                      aCCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                 >  2:145095/1‑149 (MQ=255)                                                                                                                                         aGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTc              >  5:199900/1‑149 (MQ=255)                                                                                                                                            cccAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTc           <  4:254316/149‑1 (MQ=255)                                                                                                                                            cccAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTc           <  4:136748/149‑1 (MQ=255)                                                                                                                                             ccAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                 >  3:279176/1‑142 (MQ=255)                                                                                                                                             ccAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                 <  4:279176/142‑1 (MQ=255)                                                                                                                                             ccAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTcc          <  6:252704/149‑1 (MQ=255)                                                                                                                                              cAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCa         <  5:125096/149‑1 (MQ=255)                                                                                                                                                gACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGc       <  6:116247/149‑1 (MQ=255)                                                                                                                                                 aCCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCGGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTGCGTGCTCGGCTTCCAGc       <  8:244508/148‑1 (MQ=255)                                                                                                                                                  ccGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                 >  5:12068/1‑137 (MQ=255)                                                                                                                                                  ccGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                 <  6:12068/137‑1 (MQ=255)                                                                                                                                                     gTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGgc  <  5:8402/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CTGCGGTGGCAGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCC‑TGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGC  >  NC_002947/1843222‑1843518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑