Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,843,970 G→A 16.6% A275V (GCG→GTG)  gacS ← sensor protein GacS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,843,9700GA16.6% 92.8 / 9.7 42A275V (GCG→GTG) gacSsensor protein GacS
Reads supporting (aligned to +/- strand):  ref base G (15/20);  new base A (4/3);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

AGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTC  >  NC_002947/1843823‑1844115
                                                                                                                                                    |                                                                                                                                                 
aGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTgcgcgc                                                                                                                                                  >  4:235141/1‑149 (MQ=255)
 ggTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGcc                                                                                                                                                 <  1:178193/149‑1 (MQ=255)
   tAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCACGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCAt                                                                                                                                               <  6:331605/149‑1 (MQ=255)
      tCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTc                                                                                                                                            <  4:145841/149‑1 (MQ=255)
        gAGCTGGCGCGGTGTCATCTCGCTTTTTTTCAGCAGGTGGGCAAAGCCGAGGATGCCCTTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCa                                                                                                                                          <  5:68836/149‑1 (MQ=255)
         aGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAg                                                                                                                                         >  5:141029/1‑149 (MQ=255)
            tGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTc                                                                                                                                      >  2:229123/1‑149 (MQ=255)
              gcgcGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGa                                                                                                                                    >  8:194627/1‑149 (MQ=255)
                    tgtCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCt                                                                                                                              <  6:141029/149‑1 (MQ=255)
                     gtCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCACCATGTCCAGCTCGATGTTCTg                                                                                                                             >  3:170971/1‑149 (MQ=255)
                         tctcGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCGAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGAtt                                                                                                                         >  1:158354/1‑149 (MQ=255)
                           tcGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTc                                                                                                                       >  7:265660/1‑149 (MQ=255)
                              cTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCGAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGAt                                                                                                                    <  2:158354/149‑1 (MQ=255)
                               ttttttGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCAAGTCCAGCTCGATGTACTGGATTTCGaag                                                                                                                   >  2:283388/1‑147 (MQ=255)
                                   ttGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGCTCTGGATTTCGATGGtt                                                                                                                <  1:229123/148‑1 (MQ=255)
                                      cagcagGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCa                                                                                                            <  5:129248/149‑1 (MQ=255)
                                       agcagGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGAttt                                                                                                                        <  6:209150/136‑1 (MQ=255)
                                             tGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTg                                                                                                     <  2:6249/149‑1 (MQ=255)
                                               ggTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGgc                                                                                                   >  8:16441/1‑149 (MQ=255)
                                               ggTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCACCATGTCCAGCTCGATGTTCTGGATTTCGAt                                                                                                                    >  8:119596/1‑132 (MQ=255)
                                               ggTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCACCATGTCCAGCTCGATGTTCTGGATTTCGAt                                                                                                                    <  7:119596/132‑1 (MQ=255)
                                                        gAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCACCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGTTTCTGGCGCACGTCtt                                                                                          >  8:215950/1‑149 (MQ=255)
                                                         aGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTc                                                                                         <  5:90570/149‑1 (MQ=255)
                                                              gacATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAg                                                                                                           <  5:12508/124‑1 (MQ=255)
                                                              gacATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAg                                                                                                           >  6:12508/3‑126 (MQ=255)
                                                                     cgCGGGCTGAGTATGTCGTTGATCATGTTGGCGATG‑TCTCACGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGt                                                                              <  1:283388/147‑1 (MQ=255)
                                                                     aGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGt                                                                              <  7:194627/148‑1 (MQ=255)
                                                                           gTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCt                                                                       >  5:285757/1‑149 (MQ=255)
                                                                               ggATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTgtgt                                                                   >  4:329762/1‑149 (MQ=255)
                                                                                                         aaCTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCACCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAg                                         <  7:215950/149‑1 (MQ=255)
                                                                                                          aCTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTg                                                     >  4:1912/1‑136 (MQ=255)
                                                                                                          aCTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTg                                                     <  3:1912/136‑1 (MQ=255)
                                                                                                               gcaCTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGGGTTGCAGTGCTTCGTGGGCATTCTGCAgggat                                     >  6:111086/3‑146 (MQ=255)
                                                                                                                gACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGCTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTc                                   <  4:32582/149‑1 (MQ=255)
                                                                                                                  cTTGATGCGGCTGGCCTCAAGTGACGCTGTGCGCGCCAAGTCCAGCTCGATGTTCTGGAGTTCGATGGTGTCCAGGGGCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCCGGGTTGCgg                                 <  5:111086/149‑1 (MQ=255)
                                                                                                                     gATGCGGCTGGCCTCAAGTGCCTCTTTGCGCACCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGcc                               <  4:170971/148‑1 (MQ=255)
                                                                                                                              ggCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGa                     >  2:319729/1‑149 (MQ=255)
                                                                                                                                 cTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGc                  >  2:262500/1‑149 (MQ=255)
                                                                                                                                  tCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGcc                 >  7:30985/1‑149 (MQ=255)
                                                                                                                                      gTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTgg             <  6:285757/149‑1 (MQ=255)
                                                                                                                                      gTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTgg             <  3:235141/149‑1 (MQ=255)
                                                                                                                                      gTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTgg             <  8:265660/149‑1 (MQ=255)
                                                                                                                                             tttGAGCACCATGTCCAGATCGATGTTCTTGATTTCGATGGTTTCCAGTTTCTGGCGAACGTATTCTGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTAGTGGGCATTATGCAGGGTTTCGGCAATGCGGTTGATGCAGTGGGAAAGtt      >  7:105515/1‑149 (MQ=255)
                                                                                                                                                 cgcgcCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTc  <  7:16441/149‑1 (MQ=255)
                                                                                                                                                 cgcgcCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTc  <  1:319729/149‑1 (MQ=255)
                                                                                                                                                 cgcgcCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTATTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTc  >  3:57095/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGGTAGTCGAGCTGGCGCGGTGTCATCTCGCTTTTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTC‑CGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCAGCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTC  >  NC_002947/1843823‑1844115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑