| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,859,529 | T→A | 8.3% | intergenic (‑121/+35) | PP_1662 ← / ← PP_1663 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,859,529 | 0 | T | A | 8.3% | 172.3 / 5.6 | 60 | intergenic (‑121/+35) | PP_1662/PP_1663 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (26/29); new base A (2/3); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
AAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTCCC > NC_002947/1859383‑1859670 | aaGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTgg < 7:33820/149‑1 (MQ=255) tGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCCCAGTGCGGGGCt > 4:109868/1‑149 (MQ=255) tGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCt > 6:309436/1‑149 (MQ=255) gACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCtt > 1:311211/1‑147 (MQ=255) aCAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAg < 2:304890/149‑1 (MQ=255) cAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCCCAGTGCGGGGCttttct < 3:109868/149‑5 (MQ=255) tgtgtCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCCCAGTGCGGGGCttttctttg < 5:28578/149‑8 (MQ=255) tgtCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTg < 1:115417/149‑1 (MQ=255) tCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgca < 5:213664/149‑1 (MQ=255) tCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgca < 4:287895/149‑1 (MQ=255) tCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgc < 3:27367/148‑1 (MQ=255) tCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGATCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTg > 7:73595/1‑147 (MQ=255) gCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTgcagg < 5:228371/149‑1 (MQ=255) tttATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCCCAGTGCGGGGc > 8:58191/1‑124 (MQ=255) tttATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCCCAGTGCGGGGc < 7:58191/124‑1 (MQ=255) tataGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGTATGATGCCTGGGTGGGCTTTTTCGCAGAAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGAt > 7:158852/1‑148 (MQ=255) ataGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCTTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGAt > 6:180115/1‑147 (MQ=255) taGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAg > 1:167303/1‑149 (MQ=255) taGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGAAGCAAAGAAAAGCACCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAg > 5:68963/1‑149 (MQ=255) gagTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTgcgc > 6:125508/1‑141 (MQ=255) gagTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTgcgc < 5:125508/141‑1 (MQ=255) agTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTg < 3:29672/149‑1 (MQ=255) aGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGAGCGATCAGTTGACCTTCAc > 5:260117/1‑149 (MQ=255) aGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATCAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCAc > 2:4059/1‑149 (MQ=255) cTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCtt < 2:311211/149‑1 (MQ=255) agtaagtaAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGcc < 2:167303/149‑1 (MQ=255) agtaaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATc > 5:159651/1‑148 (MQ=255) gtaaAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCACGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGGTTGCCATCGa > 7:280177/1‑149 (MQ=255) aaaCAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGAcc < 8:73595/148‑1 (MQ=255) aaCAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGt < 7:310097/149‑1 (MQ=255) ttcATGAAACTTGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGtt > 4:250031/3‑118 (MQ=255) ggCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCa < 6:102314/149‑1 (MQ=255) gCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGcc > 4:119966/1‑120 (MQ=255) gCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGcc < 3:119966/120‑1 (MQ=255) tGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGa > 5:25215/1‑108 (MQ=255) tGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGa < 6:25215/108‑1 (MQ=255) aaaCAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTg < 6:5159/148‑1 (MQ=255) aaCAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgca < 5:309436/149‑1 (MQ=255) aCAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgcag > 6:41964/1‑149 (MQ=255) aaGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgcagca < 5:36513/149‑1 (MQ=255) tttCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGa > 2:20552/1‑149 (MQ=255) gAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATcaca > 7:135929/1‑148 (MQ=255) tCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTcc > 8:34065/1‑149 (MQ=255) aaGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTg < 8:158852/149‑1 (MQ=255) aGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGc > 4:284616/1‑149 (MQ=255) tgctgcCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCg < 3:284616/149‑1 (MQ=255) tgcCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTgcg > 3:124502/1‑73 (MQ=255) tgcCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTgcg < 4:124502/73‑1 (MQ=255) ccTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgc > 2:198091/1‑117 (MQ=255) ccTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTgc < 1:198091/117‑1 (MQ=255) tgggCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTg > 2:65463/1‑149 (MQ=255) tgggCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTg > 4:174051/1‑149 (MQ=255) caAAGAAACGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCCGGGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACcag < 8:135929/149‑1 (MQ=255) aGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCg < 2:292082/120‑1 (MQ=255) aGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACcagcag < 6:159651/149‑1 (MQ=255) gAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGt < 6:272032/121‑1 (MQ=255) gAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGt > 5:272032/1‑121 (MQ=255) gAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAgg < 5:41964/149‑1 (MQ=255) cccGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGAGCGTGCCATCCTGCAGCACGATCAGATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTccc < 4:42718/149‑1 (MQ=255) ccGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTcc > 6:311171/1‑147 (MQ=255) | AAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCAGGTATTGCAGGCAGTGCGCGATCAGTTGCCCTTCACCGGCTTGCCATCGACCGTGCCATCCTGCAGCACGATCACATACTCCTTGCCATCGGTCTCTACCTGGCGCAGTTGCACCAGCAGGTAGTCCC > NC_002947/1859383‑1859670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |