Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,892,225 C→A 9.0% P156Q (CCG→CAG)  PP_1697 → transcriptional regulator, GntR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,892,2250CA 9.0% 165.4 / 4.9 55P156Q (CCG→CAG) PP_1697transcriptional regulator, GntR family
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base A (2/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TCGAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAA  >  NC_002947/1892078‑1892364
                                                                                                                                                   |                                                                                                                                           
tCGAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCg                                                                                                                                            <  3:179914/149‑1 (MQ=255)
tCGAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCg                                                                                                                                            <  5:344615/149‑1 (MQ=255)
  gAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCgct                                                                                                                                          >  3:190341/1‑149 (MQ=255)
    gTCGCTGCCGGCGCCTTCCGCGGTGGCCGACGCATTGGGCCTGGCAGGGGAGGCAGTAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCGGAAGACAGCTGGCTGCCGCGCCAAACCTGCCAGCCgctgc                                                                                                                                        >  8:298008/1‑149 (MQ=255)
          gCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCAGCGGCTGGaag                                                                                                                                  <  7:118192/149‑3 (MQ=255)
           ccGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGAc                                                                                                                                   <  6:333739/147‑1 (MQ=255)
             ggCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTgg                                                                                                                               <  7:67076/149‑1 (MQ=255)
                  cTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTACTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGt                                                                                                                                 <  1:300852/142‑1 (MQ=255)
                  cTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTACTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGt                                                                                                                                 >  2:300852/1‑142 (MQ=255)
                   ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGTTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTg                                                                                                                          >  2:274408/1‑148 (MQ=255)
                   ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCt                                                                                                                           <  5:25248/147‑1 (MQ=255)
                   ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCt                                                                                                                           >  6:25248/1‑147 (MQ=255)
                           tGGGCGAGGCATTGGGGGTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGGTGCCGCGCGAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAg                                                                                                                 <  5:46621/149‑1 (MQ=255)
                            ggCCGAGTCATTGGGCCTGTCAGGGGATGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAgg                                                                                                                <  7:298008/149‑1 (MQ=255)
                                gAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATc                                                                                                                            <  6:167081/133‑1 (MQ=255)
                                gAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAAACTTCCAGCCGCTGCTGGACGTGGATc                                                                                                                            >  5:167081/1‑133 (MQ=255)
                                     aTTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgc                                                                                                       >  1:75847/1‑149 (MQ=255)
                                      ttGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgct                                                                                                      >  8:302709/1‑149 (MQ=255)
                                           ccTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTAcc                                                                                                 <  2:75847/149‑1 (MQ=255)
                                            cTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTAccc                                                                                                >  5:106376/1‑149 (MQ=255)
                                                 aGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATc                                                                                            >  2:316165/1‑148 (MQ=255)
                                                      aGGCAGAAGTGATCCGCATCATTCGCACACGTCTGTTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGaa                                                                                      <  1:274408/149‑1 (MQ=255)
                                                          aGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTgg                                                                                                                               >  3:189121/1‑104 (MQ=255)
                                                          aGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTgg                                                                                                                               <  4:189121/104‑1 (MQ=255)
                                                                   ccGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGt                                                                          >  2:295720/1‑148 (MQ=255)
                                                                    cGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGt                                                                          >  3:130006/1‑147 (MQ=255)
                                                                             cGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGc                                                               >  6:3743/1‑149 (MQ=255)
                                                                              gCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAggtg                                                                    <  7:199594/143‑1 (MQ=255)
                                                                              gCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAggtg                                                                    >  8:199594/1‑143 (MQ=255)
                                                                              gCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGc                                                               <  4:130006/148‑1 (MQ=255)
                                                                                acacGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTa                                                            >  6:33955/1‑149 (MQ=255)
                                                                                  acGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACg                                                          <  6:99841/149‑1 (MQ=255)
                                                                                  acGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACg                                                          >  5:99841/1‑149 (MQ=255)
                                                                                         cTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCga                                                      <  7:28874/146‑1 (MQ=255)
                                                                                         cTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCAGCCGCGCCAAACCTTCCAGCCGCTGCTGGACTTGGATCTGAACAGCGTGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCgaa                                                     <  4:286143/147‑1 (MQ=255)
                                                                                          tCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCgaaga                                                   >  1:171756/1‑148 (MQ=255)
                                                                                            ggCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGaaa                                                 <  7:302709/148‑1 (MQ=255)
                                                                                                 ccAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACACGATCTACGAAgcc                                                                                   >  6:163604/1‑108 (MQ=255)
                                                                                                     ccGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               >  2:333058/1‑141 (MQ=255)
                                                                                                     ccGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               <  1:333058/141‑1 (MQ=255)
                                                                                                     ccGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGAccgc                                       <  1:316165/149‑1 (MQ=255)
                                                                                                                cGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTc                             >  3:250827/1‑148 (MQ=255)
                                                                                                                       aTCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGt                     <  2:171756/149‑1 (MQ=255)
                                                                                                                            gCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCAt                 <  5:33955/148‑1 (MQ=255)
                                                                                                                              tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               >  3:26537/1‑116 (MQ=255)
                                                                                                                              tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               <  4:26537/116‑1 (MQ=255)
                                                                                                                              tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGc              >  1:82493/1‑149 (MQ=255)
                                                                                                                                     ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATg                <  4:188689/140‑1 (MQ=255)
                                                                                                                                     ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATg                >  3:188689/1‑140 (MQ=255)
                                                                                                                                     ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgc       >  2:39088/1‑149 (MQ=255)
                                                                                                                                      cAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgct      <  4:250827/149‑1 (MQ=255)
                                                                                                                                           cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  >  1:140304/3‑148 (MQ=255)
                                                                                                                                           cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  <  2:145285/146‑1 (MQ=255)
                                                                                                                                           cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  <  2:140304/146‑1 (MQ=255)
                                                                                                                                           cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  >  1:145285/3‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
TCGAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAA  >  NC_002947/1892078‑1892364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑