Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,892,228 T→G 9.8% L157R (CTG→CGG)  PP_1697 → transcriptional regulator, GntR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,892,2280TG 9.8% 147.4 / 6.0 51L157R (CTG→CGG) PP_1697transcriptional regulator, GntR family
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base G (2/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAA  >  NC_002947/1892080‑1892364
                                                                                                                                                    |                                                                                                                                        
gAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCgct                                                                                                                                          >  3:190341/1‑149 (MQ=255)
  gTCGCTGCCGGCGCCTTCCGCGGTGGCCGACGCATTGGGCCTGGCAGGGGAGGCAGTAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCGGAAGACAGCTGGCTGCCGCGCCAAACCTGCCAGCCgctgc                                                                                                                                        >  8:298008/1‑149 (MQ=255)
        gCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCAGCGGCTGGaag                                                                                                                                  <  7:118192/149‑3 (MQ=255)
         ccGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGAc                                                                                                                                   <  6:333739/147‑1 (MQ=255)
           ggCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTgg                                                                                                                               <  7:67076/149‑1 (MQ=255)
                cTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTACTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGt                                                                                                                                 <  1:300852/142‑1 (MQ=255)
                cTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTACTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGt                                                                                                                                 >  2:300852/1‑142 (MQ=255)
                 ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGTTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTg                                                                                                                          >  2:274408/1‑148 (MQ=255)
                 ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCt                                                                                                                           >  6:25248/1‑147 (MQ=255)
                 ttCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCt                                                                                                                           <  5:25248/147‑1 (MQ=255)
                         tGGGCGAGGCATTGGGGGTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGGTGCCGCGCGAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAg                                                                                                                 <  5:46621/149‑1 (MQ=255)
                          ggCCGAGTCATTGGGCCTGTCAGGGGATGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAgg                                                                                                                <  7:298008/149‑1 (MQ=255)
                              gAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATc                                                                                                                            <  6:167081/133‑1 (MQ=255)
                              gAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAAACTTCCAGCCGCTGCTGGACGTGGATc                                                                                                                            >  5:167081/1‑133 (MQ=255)
                                   aTTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgc                                                                                                       >  1:75847/1‑149 (MQ=255)
                                    ttGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCgctgct                                                                                                      >  8:302709/1‑149 (MQ=255)
                                         ccTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTAcc                                                                                                 <  2:75847/149‑1 (MQ=255)
                                          cTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTAccc                                                                                                >  5:106376/1‑149 (MQ=255)
                                               aGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATc                                                                                            >  2:316165/1‑148 (MQ=255)
                                                    aGGCAGAAGTGATCCGCATCATTCGCACACGTCTGTTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGaa                                                                                      <  1:274408/149‑1 (MQ=255)
                                                        aGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTgg                                                                                                                               >  3:189121/1‑104 (MQ=255)
                                                        aGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTgg                                                                                                                               <  4:189121/104‑1 (MQ=255)
                                                                 ccGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGt                                                                          >  2:295720/1‑148 (MQ=255)
                                                                  cGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGt                                                                          >  3:130006/1‑147 (MQ=255)
                                                                           cGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGc                                                               >  6:3743/1‑149 (MQ=255)
                                                                            gCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAggtg                                                                    <  7:199594/143‑1 (MQ=255)
                                                                            gCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAggtg                                                                    >  8:199594/1‑143 (MQ=255)
                                                                            gCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGc                                                               <  4:130006/148‑1 (MQ=255)
                                                                              acacGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTa                                                            >  6:33955/1‑149 (MQ=255)
                                                                                acGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACg                                                          <  6:99841/149‑1 (MQ=255)
                                                                                acGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACg                                                          >  5:99841/1‑149 (MQ=255)
                                                                                       cTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCga                                                      <  7:28874/146‑1 (MQ=255)
                                                                                       cTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCAGCCGCGCCAAACCTTCCAGCCGCTGCTGGACTTGGATCTGAACAGCGTGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCgaa                                                     <  4:286143/147‑1 (MQ=255)
                                                                                        tCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCgaaga                                                   >  1:171756/1‑148 (MQ=255)
                                                                                          ggCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGaaa                                                 <  7:302709/148‑1 (MQ=255)
                                                                                               ccAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACACGATCTACGAAgcc                                                                                   >  6:163604/1‑108 (MQ=255)
                                                                                                   ccGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               >  2:333058/1‑141 (MQ=255)
                                                                                                   ccGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               <  1:333058/141‑1 (MQ=255)
                                                                                                   ccGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGAccgc                                       <  1:316165/149‑1 (MQ=255)
                                                                                                              cGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTc                             >  3:250827/1‑148 (MQ=255)
                                                                                                                     aTCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGt                     <  2:171756/149‑1 (MQ=255)
                                                                                                                          gCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCAt                 <  5:33955/148‑1 (MQ=255)
                                                                                                                            tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               >  3:26537/1‑116 (MQ=255)
                                                                                                                            tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAAcc                                               <  4:26537/116‑1 (MQ=255)
                                                                                                                            tGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGc              >  1:82493/1‑149 (MQ=255)
                                                                                                                                   ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATg                <  4:188689/140‑1 (MQ=255)
                                                                                                                                   ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATg                >  3:188689/1‑140 (MQ=255)
                                                                                                                                   ccAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgc       >  2:39088/1‑149 (MQ=255)
                                                                                                                                    cAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTtgct      <  4:250827/149‑1 (MQ=255)
                                                                                                                                         cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  >  1:140304/3‑148 (MQ=255)
                                                                                                                                         cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  <  2:145285/146‑1 (MQ=255)
                                                                                                                                         cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  <  2:140304/146‑1 (MQ=255)
                                                                                                                                         cgtCCAGCAGCGGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCaa  >  1:145285/3‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
GAGTCGCTGCCGGCGCCTTCCGCGGTGGCCGAGGCATTGGGCCTGGCAGGGGAGGCAGAAGTGATCCGCATCATTCGCACACGTCTGCTCGGCGCCCAGCCGGTGCTGGCCGAAGAAATCTGGCTGCCGCGCCAAACCTTCCAGCCGCTGCTGGACGTGGATCTGAACAGCGAGGGCCCGCTGCTGTACCCGATCTACGAAGCGCTGTGCGGTCAGGTGGTCGCCTACGCCGAAGAAACCCTGACCGCCGAAGCGGTCGACGCGGTGCATGGCCGCTTGCTGCAA  >  NC_002947/1892080‑1892364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑