Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,918,256 C→G 7.2% intergenic (‑32/+86) PP_1718 ← / ← prc EAL domain‑containing diguanylate phosphodiesterase/tail‑specific protease Prc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,918,2560CG 7.2% 256.6 / 7.2 80intergenic (‑32/+86)PP_1718/prcEAL domain‑containing diguanylate phosphodiesterase/tail‑specific protease Prc
Reads supporting (aligned to +/- strand):  ref base C (41/33);  new base G (2/4);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCC  >  NC_002947/1918111‑1918402
                                                                                                                                                 |                                                                                                                                                  
cTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTg                                                                                                                                                  >  1:214348/1‑148 (MQ=255)
      gCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGccc                                                                                                                                           >  8:61091/1‑149 (MQ=255)
        cGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGGGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCgg                                                                                                                                         >  3:310394/1‑149 (MQ=255)
        cGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCgg                                                                                                                                         >  8:261436/1‑149 (MQ=255)
        cGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCgg                                                                                                                                         <  6:222789/149‑1 (MQ=255)
         gAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGc                                                                                                                                        <  3:93869/149‑1 (MQ=255)
         gAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGc                                                                                                                                        <  7:261436/149‑1 (MQ=255)
            tACCCTATGTTAACTCATGCACATGATTGGATGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATGCAGATGCCCGGCCCt                                                                                                                                     <  8:159464/149‑1 (MQ=255)
               ccTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCa                                                                                                                                   >  3:126383/1‑148 (MQ=255)
               ccTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAAAGGTCATGATGGATTACTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGACCTCa                                                                                                                                   >  3:17196/1‑148 (MQ=255)
                              gCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCAc                                                                                                                   >  2:123761/1‑149 (MQ=255)
                                 caTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGcc                                                                                                                                       <  7:182834/126‑1 (MQ=255)
                                 caTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGcc                                                                                                                                       >  8:182834/1‑126 (MQ=255)
                                 caTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGAt                                                                                                                >  1:322709/1‑149 (MQ=255)
                                 caTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGAt                                                                                                                <  1:123761/149‑1 (MQ=255)
                                  aTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGAt                                                                                                                >  4:251629/1‑148 (MQ=255)
                                  aTGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGAt                                                                                                                >  7:125892/1‑148 (MQ=255)
                                     aTTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGAc                                                                                                             <  1:100677/148‑1 (MQ=255)
                                     aTTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACt                                                                                                            <  4:139455/149‑1 (MQ=255)
                                     aTTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATAATTCACGACAGCACGATGAc                                                                                                             <  3:275926/148‑1 (MQ=255)
                                      ttGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCCAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGa                                                                                                                 <  4:105494/143‑1 (MQ=255)
                                      ttGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGa                                                                                                                 >  3:105494/1‑143 (MQ=255)
                                      ttGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACt                                                                                                            <  3:295449/148‑1 (MQ=255)
                                          cTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCTGAAACGGTCATGATGGATTCCTGAAAAGTAAGGGACGGGCATCTGCATGCCCGGCCGGCATATTTCCCGCCAGCATGATGACTGatt                                                                                                        >  5:278561/1‑148 (MQ=255)
                                           tGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtga                                                                                                      >  8:20009/1‑149 (MQ=255)
                                           tGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTtga                                                                                                      >  4:54579/1‑149 (MQ=255)
                                                  gACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGa                                                                                               <  3:302849/149‑1 (MQ=255)
                                                    cTGTGAATAATGCGCTGAGCCAGGATGGCACTAAAGGCAGTGAGCTGTTCGGCAACTGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGAt                                                                                              <  4:17196/148‑1 (MQ=255)
                                                            aCTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACattat                                                                                      >  4:299773/1‑148 (MQ=255)
                                                              tGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCt                                                                                   >  6:331692/1‑149 (MQ=255)
                                                              tGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCt                                                                                   >  6:326847/1‑149 (MQ=255)
                                                              tGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCt                                                                                   <  8:125892/149‑1 (MQ=255)
                                                                 gcTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGcc                                                                                <  7:61091/149‑1 (MQ=255)
                                                                  cTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCca                                                                               <  4:126383/149‑1 (MQ=255)
                                                                       ccAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCAtt                                                                          <  6:215727/149‑1 (MQ=255)
                                                                             tGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAg                                                                    <  3:54579/149‑1 (MQ=255)
                                                                               gCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGt                                                                  >  7:77626/1‑149 (MQ=255)
                                                                                     aaGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTa                                                            >  8:297092/1‑149 (MQ=255)
                                                                                     aaGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTACCGCCAGCACGATGACTGTTTCATGACACAGCACATAATTCTGACATCATTGCTTAGGTGCCtt                                                             >  7:59850/1‑148 (MQ=255)
                                                                                      aGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTAc                                                           >  5:65838/1‑149 (MQ=255)
                                                                                              ggCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTg                                                     <  4:310394/146‑1 (MQ=255)
                                                                                              aGCTGTTCTGCAACGGTCATTATGGATTCCTGAAAATTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTg                                                     <  8:201854/147‑1 (MQ=255)
                                                                                              aGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCAAATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGc                                                                <  3:321021/136‑1 (MQ=255)
                                                                                              aGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCAAATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGc                                                                >  4:321021/1‑136 (MQ=255)
                                                                                                cTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCa                                                 <  3:226341/149‑1 (MQ=255)
                                                                                                   ttCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCt                                                              <  2:239745/133‑1 (MQ=255)
                                                                                                   ttCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCt                                                              >  1:239745/1‑133 (MQ=255)
                                                                                                        cAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTg                                         <  8:77626/149‑1 (MQ=255)
                                                                                                          aCGGTCATTATGTATTCCTTAAATTTATGGGCCGGGCATGTGCATGCCGGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCt                                       <  2:322709/149‑1 (MQ=255)
                                                                                                           cGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTg                                      <  7:20009/149‑1 (MQ=255)
                                                                                                           cGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTg                                      <  7:50745/149‑1 (MQ=255)
                                                                                                           cGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTg                                      <  6:65838/149‑1 (MQ=255)
                                                                                                             gTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTgg                                     >  6:253923/1‑148 (MQ=255)
                                                                                                             gTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGc                                    <  5:326847/149‑1 (MQ=255)
                                                                                                             gTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGc                                    <  5:331692/149‑1 (MQ=255)
                                                                                                                    tgGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAg                              <  3:299773/148‑1 (MQ=255)
                                                                                                                       aTTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAg                          >  2:132792/1‑149 (MQ=255)
                                                                                                                           cTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTg                                      <  3:269536/133‑1 (MQ=255)
                                                                                                                           cTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTg                                      >  4:269536/1‑133 (MQ=255)
                                                                                                                            tGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGGCTTACTTCTTGGCCACGTTGGTGCTGGCACTGAGGTAGTCGAt                     >  4:185459/1‑149 (MQ=255)
                                                                                                                            tGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCGCTGAGGTAGTCGAt                     >  3:59563/1‑149 (MQ=255)
                                                                                                                            tGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGa                      >  1:41916/1‑148 (MQ=255)
                                                                                                                            tGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGAt                     >  3:149992/1‑149 (MQ=255)
                                                                                                                            tGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGAt                     >  6:295758/1‑149 (MQ=255)
                                                                                                                               aaaGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAg                                                                    <  4:259183/99‑1 (MQ=255)
                                                                                                                               aaaGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAg                                                                    >  3:259183/1‑99 (MQ=255)
                                                                                                                               aaaGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGt                                                                  <  1:212410/101‑1 (MQ=255)
                                                                                                                               aaaGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATAATTGCTGAGGt                                                                  >  2:212410/1‑101 (MQ=255)
                                                                                                                                   tAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATg              >  2:80445/1‑149 (MQ=255)
                                                                                                                                    aaGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGc             >  7:179954/1‑149 (MQ=255)
                                                                                                                                      gggCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAgg                 >  4:260828/1‑143 (MQ=255)
                                                                                                                                      gggCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAgg                 <  3:260828/143‑1 (MQ=255)
                                                                                                                                       ggCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGc             >  6:315300/1‑146 (MQ=255)
                                                                                                                                       ggCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGc             <  5:315300/146‑1 (MQ=255)
                                                                                                                                        gCCGGGCATGCAGATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTAATTATTGGCCACCTTGGTGCTGGCTCTGAGGTAGTCGATCAGGATGCGccc         >  4:176722/1‑149 (MQ=255)
                                                                                                                                        gCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGAt                     >  6:341690/1‑137 (MQ=255)
                                                                                                                                        gCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGAt                     <  5:341690/137‑1 (MQ=255)
                                                                                                                                          cGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCCACCgg       >  4:116731/1‑149 (MQ=255)
                                                                                                                                          cGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCAACATTGCTGAGGTGCCTTAATTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCgg       >  1:109780/1‑149 (MQ=255)
                                                                                                                                               aTCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTcc  >  3:22513/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTCATGGCCGAATACCCTATGTTCACTCAGGCACATGATTGGCTGGAACAGACTGTGAATACTGCGCTGAGCCAGGATGGCACTCAAGGCACTGAGCTGTTCGGCAACGGTCATGATGGATTCCTGAAAAGTAAGGGCCGGGCATCTGCATGCCCGGCCCTCATATTTCACGACAGCACGATGACTGTTTGATGACAGATCACATTATTCTGCCATCATTGCTGAGGTGCCTTACTTCTTGGCCACCTTGGTGCTGGCACTGAGGTAGTCGATCAGGATGCGACCGGTTTCC  >  NC_002947/1918111‑1918402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑