Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,945,325 T→A 5.8% L69Q (CTG→CAG)  PP_1745 → short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,945,3250TA 5.8% 211.5 / 3.2 69L69Q (CTG→CAG) PP_1745short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (29/36);  new base A (2/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

AGCGCTGGGCCCGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAATCTGATGGGCGTG  >  NC_002947/1945178‑1945461
                                                                                                                                                   |                                                                                                                                        
aGCGGTGGGCCGGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTg                                                                                                                                         <  8:220402/149‑1 (MQ=255)
     tGGGCCCGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGcc                                                                                                                                      >  4:127326/1‑147 (MQ=255)
         cccGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGcc                                                                                                                                >  3:116918/1‑149 (MQ=255)
         cccGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGcc                                                                                                                                >  4:195066/1‑149 (MQ=255)
         cccGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGcc                                                                                                                                >  1:157077/1‑149 (MQ=255)
           cGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTg                                                                                                                              <  3:304524/149‑1 (MQ=255)
               aGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTgcgaa                                                                                                                          <  6:156797/149‑1 (MQ=255)
                gggCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTgcgaag                                                                                                                         <  7:134069/149‑1 (MQ=255)
                gggCTGGCGCCTGGAATTGGCCGATGTCAACGAAGCCGGACTGGGCGTAACGGTGGAGCTGGTTCGCCAGGCGGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGGGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTgcgaa                                                                                                                          <  6:232864/148‑1 (MQ=255)
                 ggCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTgcgaagc                                                                                                                        >  6:326580/1‑149 (MQ=255)
                  gCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTgcgaagcg                                                                                                                       <  1:68719/149‑1 (MQ=255)
                    tGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTgcgaagcgaa                                                                                                                     <  2:157077/149‑1 (MQ=255)
                          cTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCgg                                                                                                               >  3:129182/1‑149 (MQ=255)
                               aTTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTgggg                                                                                                          >  1:221398/1‑149 (MQ=255)
                               aTTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGCTgggg                                                                                                          >  7:154717/1‑149 (MQ=255)
                               aTTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCAGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTgggg                                                                                                          >  3:10510/1‑149 (MQ=255)
                                ttGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCAGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGt                                                                                                         <  4:10510/149‑1 (MQ=255)
                                    ccGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGAt                                                                                                     <  2:78473/149‑1 (MQ=255)
                                        tGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGa                                                                                                 <  3:127326/149‑1 (MQ=255)
                                        tGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGa                                                                                                 <  7:267990/149‑1 (MQ=255)
                                            aaCGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGt                                                                                             <  4:59517/149‑1 (MQ=255)
                                            aaCGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGt                                                                                             >  8:136425/1‑149 (MQ=255)
                                             aCGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTc                                                                                            <  4:129182/149‑1 (MQ=255)
                                             aCGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTc                                                                                            <  8:235833/149‑1 (MQ=255)
                                                          gcgcGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCg                                                                               >  7:215503/1‑149 (MQ=255)
                                                             cgAAACGCTGGGGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCg                                                                            >  5:241430/1‑149 (MQ=255)
                                                              gAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGc                                                                           >  6:191411/1‑149 (MQ=255)
                                                                   gCTGGGGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTcgg                                                                      <  6:241430/149‑1 (MQ=255)
                                                                    cTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGGGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTcggg                                                                     <  3:20896/149‑1 (MQ=255)
                                                                      ggAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTcgggcg                                                                   >  6:336279/1‑149 (MQ=255)
                                                                          cAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGct                                                               >  7:79207/1‑149 (MQ=255)
                                                                             gTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCtt                                                            >  6:6444/1‑149 (MQ=255)
                                                                                 gCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGTGCTCTTCGcc                                                        <  8:215503/149‑1 (MQ=255)
                                                                                  ccAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGc                                                                                                                                 >  6:265162/1‑75 (MQ=255)
                                                                                  ccAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGcc                                                        >  5:198472/1‑148 (MQ=255)
                                                                                      gCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACt                                                   >  3:200900/1‑149 (MQ=255)
                                                                                        cGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTc                                                           <  3:303017/139‑1 (MQ=255)
                                                                                        cGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTc                                                           >  4:303017/1‑139 (MQ=255)
                                                                                        cGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGa                                                 >  6:75769/1‑149 (MQ=255)
                                                                                        cGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGa                                                 <  5:6444/149‑1 (MQ=255)
                                                                                        cGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGa                                                 <  5:75769/149‑1 (MQ=255)
                                                                                        cGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCAGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGa                                                 >  3:305795/1‑149 (MQ=255)
                                                                                        cGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCAGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGa                                                 >  3:300931/1‑149 (MQ=255)
                                                                                             gCGACGGCTTCGTCCATCTCTGCGATGTGCGCGAGTATAGCCAGCTGACGGCCCTGGACCAGGCCTGCGAAGCGAAGTTCGTTGGGGTAGATGTGATCGTCAACAACGCCGGCGTCTCTTCGGGCGGGCTCTTCGCCGAACTGACGCTg                                            <  8:79207/149‑1 (MQ=255)
                                                                                               gACGGCTTCGTCCAGCGCTGCGATGTGCGCGAGTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTgga                                          <  2:139046/149‑1 (MQ=255)
                                                                                                aCGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTgga                                          <  2:231064/148‑1 (MQ=255)
                                                                                                aCGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTg                                            >  1:231064/1‑146 (MQ=255)
                                                                                                  ggCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTggagga                                       >  1:275030/1‑149 (MQ=255)
                                                                                                    cTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAggact                                     >  6:185860/1‑149 (MQ=255)
                                                                                                    cTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAggact                                     >  6:67194/1‑149 (MQ=255)
                                                                                                       cGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAggactgg                                   <  5:185860/148‑1 (MQ=255)
                                                                                                       cGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAggactgg                                   <  8:154717/148‑1 (MQ=255)
                                                                                                       cGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAggactgg                                   <  3:195066/148‑1 (MQ=255)
                                                                                                          ccAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCACTGGCCCAGGCCTGCGAAGCGAAGTTCGCTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTCACGCTGGAcgacagggact                               >  8:293590/1‑149 (MQ=255)
                                                                                                           cAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGATGGAggactgggactg                              >  2:304388/1‑149 (MQ=255)
                                                                                                            aGCGCTGCGATGTGCGCGACTTTAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGGTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGgactgggactgg                             <  7:293590/149‑1 (MQ=255)
                                                                                                                   cGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATc                       <  3:288218/148‑1 (MQ=255)
                                                                                                                   cGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATc                       <  3:298922/148‑1 (MQ=255)
                                                                                                                     aTGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATcgcg                    <  2:275030/149‑1 (MQ=255)
                                                                                                                     aTGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATcgcg                    <  5:191411/149‑1 (MQ=255)
                                                                                                                     aTGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATcgcg                    <  2:221398/149‑1 (MQ=255)
                                                                                                                        tgCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGGCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGGCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGt                  <  5:326580/148‑1 (MQ=255)
                                                                                                                        tgCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGt                  <  5:336279/148‑1 (MQ=255)
                                                                                                                           gcgACTATAGCCAGCTGACGGCCCAGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAAt              <  4:305795/149‑1 (MQ=255)
                                                                                                                           gcgACTATAGCCAGCTGACGGCCCAGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAAt              <  4:300931/149‑1 (MQ=255)
                                                                                                                                    gCCAGCTGACGGGCCTGGGACAGGCCTTCTAAGCGAAGTTGGGTGGGGTCGATGTGATGGTCAACAACGCCGGCGTCGCTTGGGGTGGGGTGTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAATCTGATGGGc     <  4:116918/149‑1 (MQ=255)
                                                                                                                                    gCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAATCTGATGGGc     >  8:225648/1‑149 (MQ=255)
                                                                                                                                     ccAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAATCTGATGGGCg    >  7:182916/1‑149 (MQ=255)
                                                                                                                                       aGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAATCTGATGGGCGTg  <  6:204459/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
AGCGCTGGGCCCGTGAGGGCTGGCGCCTGGCATTGGCCGATGTCAACGAAGCCGGACTGCGCGAAACGCTGGAGCAGGTTCGCCAGGCCGGTGGCGACGGCTTCGTCCAGCGCTGCGATGTGCGCGACTATAGCCAGCTGACGGCCCTGGCCCAGGCCTGCGAAGCGAAGTTCGGTGGGGTCGATGTGATCGTCAACAACGCCGGCGTCGCTTCGGGCGGGCTCTTCGCCGAACTGACGCTGGAGGACTGGGACTGGCAGATCGCGGTCAATCTGATGGGCGTG  >  NC_002947/1945178‑1945461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑