Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,948,186 C→G 8.2% R490P (CGC→CCC) ‡ PP_1749 ← acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,948,1860CG 8.2% 150.8 / 5.0 49R490P (CGC→CCC) ‡PP_1749acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (23/22);  new base G (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

TGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTGGCGCACGCTGT  >  NC_002947/1948046‑1948333
                                                                                                                                            |                                                                                                                                                    
tGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccg                                                                                                                                              >  2:74115/1‑149 (MQ=255)
 gAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGCGCCTggccgt                                                                                                                                             >  3:198344/1‑148 (MQ=255)
    gTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGGACGGCAGCATCGTCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGGACGGCCAGGCGCTGgcgggccg                                                                                                                                          <  4:95623/149‑1 (MQ=255)
       cAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGCGCCTGGCcgtgcggc                                                                                                                                        <  4:198344/148‑1 (MQ=255)
                caccGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACAcgtcgt                                                                                                                              <  8:119787/149‑1 (MQ=255)
                  ccGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCa                                                                                                                            >  2:81435/1‑149 (MQ=255)
                     gAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCTGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAg                                                                                                                         <  5:97733/149‑1 (MQ=255)
                     gAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAg                                                                                                                         >  2:81953/1‑149 (MQ=255)
                          tCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAAc                                                                                                                    >  7:123622/1‑149 (MQ=255)
                           ccAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACg                                                                                                                   >  4:186883/1‑149 (MQ=255)
                              gTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccggcc                                                                                                                                           <  8:162417/122‑1 (MQ=255)
                              gTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccggcc                                                                                                                                           >  7:162417/1‑122 (MQ=255)
                                   cGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTgc                                                                                                           >  4:295511/1‑149 (MQ=255)
                                   cGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTgc                                                                                                           >  3:188906/1‑149 (MQ=255)
                                         gcgcgcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTgcgcgc                                                                                                       >  2:196087/1‑147 (MQ=255)
                                             gcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTg                                                                                                 <  3:114211/149‑1 (MQ=255)
                                                cGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTgcg‑c                                                                                             <  1:74115/149‑1 (MQ=255)
                                                     gcaTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTGCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCTGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCTACGCCCGCTGCGCGCAAGGTGCG‑CTCGGt                                                                                        <  6:78075/149‑1 (MQ=255)
                                                       aTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCg                                                                                          >  7:25378/1‑145 (MQ=255)
                                                       aTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCg                                                                                          <  8:25378/145‑1 (MQ=255)
                                                           gCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTtcgtc                                                                                  >  6:62758/1‑149 (MQ=255)
                                                           gCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTtcgtc                                                                                  <  4:188906/149‑1 (MQ=255)
                                                           gCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTtcgtc                                                                                  <  6:21158/149‑1 (MQ=255)
                                                                     ggTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCgg                                                                         >  6:236378/1‑148 (MQ=255)
                                                                         cAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATg                                                                     <  8:298046/148‑1 (MQ=255)
                                                                         cAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGc                                                                    >  2:322878/1‑149 (MQ=255)
                                                                            gCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGc                                                                 >  1:229974/1‑149 (MQ=255)
                                                                              gTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTtcgtcg                                                                                 <  2:173464/131‑1 (MQ=255)
                                                                              gTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGctc                                                               >  4:313333/1‑149 (MQ=255)
                                                                                 cGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCtctc                                                             <  1:81435/148‑1 (MQ=255)
                                                                                        gTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCgg                                                     >  1:34395/1‑149 (MQ=255)
                                                                                           ctccAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTgg                                                  >  1:185010/1‑149 (MQ=255)
                                                                                             ccAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTgg                                                  >  7:107755/1‑147 (MQ=255)
                                                                                              cAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAg                                               <  1:196087/149‑1 (MQ=255)
                                                                                                  gTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTg                                           <  3:186883/149‑1 (MQ=255)
                                                                                                      cgccgGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGt                                       >  5:107115/1‑149 (MQ=255)
                                                                                                       gccgGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTc                                      <  3:295511/149‑1 (MQ=255)
                                                                                                           gtgtGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCg                                                            <  8:6959/123‑1 (MQ=255)
                                                                                                           gtgtGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCg                                                            >  7:6959/1‑123 (MQ=255)
                                                                                                                 aGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTg                            <  5:62758/149‑1 (MQ=255)
                                                                                                                       gCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCCTTTGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCtt                       <  1:106014/149‑1 (MQ=255)
                                                                                                                          gTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGAt                   <  1:81953/149‑1 (MQ=255)
                                                                                                                            gcgcCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATc                  >  6:213626/1‑148 (MQ=255)
                                                                                                                                cggccGGCCAGCGCCTGGCCGTGCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTg             >  4:21569/5‑149 (MQ=255)
                                                                                                                                 ggccGGCCAGCGCCTGGCCGTGCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTgg            <  3:21569/146‑1 (MQ=255)
                                                                                                                                      gCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGCCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTGGCGCAc       >  4:141659/1‑149 (MQ=255)
                                                                                                                                         aGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTGGCGCACGCt    >  3:194452/1‑149 (MQ=255)
                                                                                                                                         aGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTGGCGCACGCt    <  2:123772/149‑1 (MQ=255)
                                                                                                                                          ggCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTGGCGCACGCtg   <  2:185010/149‑1 (MQ=255)
                                                                                                                                           gcgcTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTGGCGCACGCtgt  >  4:215019/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
TGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCG‑CTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAACTGGCGCACGCTGT  >  NC_002947/1948046‑1948333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑