Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,969,467 C→T 11.9% R18H (CGC→CAC) ‡ ubiG ← ubiquinone biosynthesis O‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,969,4670CT11.9% 126.3 / 6.9 50R18H (CGC→CAC) ‡ubiGubiquinone biosynthesis O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (22/22);  new base T (2/4);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

GGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTT  >  NC_002947/1969321‑1969604
                                                                                                                                                  |                                                                                                                                         
gggCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCgg                                                                                                                                         <  6:157021/149‑1 (MQ=255)
   ccATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTgg                                                                                                                                      <  2:244806/149‑1 (MQ=255)
     aTGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGc                                                                                                                                    <  1:37857/149‑1 (MQ=255)
        gCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCGCTGGTgggacc                                                                                                                                  <  1:180203/148‑4 (MQ=255)
         cTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCGCTGGTgggaccgc                                                                                                                                <  7:131142/149‑6 (MQ=255)
         cTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCGCTGGTgggac                                                                                                                                   >  4:97869/1‑144 (MQ=255)
         cTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCGCTGGTgggac                                                                                                                                   <  3:97869/146‑3 (MQ=255)
         cTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAgc                                                                                                                                >  7:296842/1‑149 (MQ=255)
             gCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGGTGACGCGCAGCGGGTTGATTTCGTGGATCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCt                                                                                                                             <  7:32471/148‑1 (MQ=255)
                   gATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGaa                                                                                                                        <  7:305746/147‑1 (MQ=255)
                   gATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGaa                                                                                                                        >  8:305746/1‑147 (MQ=255)
                     tGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTg                                                                                                                    >  5:23058/1‑149 (MQ=255)
                      gccgccACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTgg                                                                                                                   >  1:297801/1‑149 (MQ=255)
                        cgccACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCAAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGc                                                                                                                  >  8:215991/1‑148 (MQ=255)
                        agccACCGCAACCCACGTCCAGCAGTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCAAGTTGACGCGCAGAGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGAGGTCCCACCAGCGGTGGGCCAGAGCTTCGAACTTGGc                                                                                                                  >  2:211121/2‑148 (MQ=255)
                         gccACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGa                                                                                                                >  3:133813/1‑149 (MQ=255)
                             ccGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTc                                                                                                            >  2:100733/1‑149 (MQ=255)
                                   cccACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGcgcg                                                                                                      >  3:60881/1‑149 (MQ=255)
                                   cccACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGcgcg                                                                                                      >  5:223353/1‑149 (MQ=255)
                                    ccACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCTGGCCCACCGCTGGTGGGACcgcgaaagc                                                                                                                          <  7:312549/128‑6 (MQ=38)
                                    ccACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCTGGCCCACCGCTGGTGGGACcgcgaaagc                                                                                                                          >  8:312549/1‑123 (MQ=38)
                                      aCGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTc                                                                                                   <  6:223353/149‑1 (MQ=255)
                                            aGCACTTTCTTGCCGGCCAGGCAGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGtt                                                                                             >  7:50578/1‑149 (MQ=255)
                                             gCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTg                                                                                            <  6:287768/149‑1 (MQ=255)
                                               aCTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCt                                                                                          <  4:60881/149‑1 (MQ=255)
                                                 tttCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCa                                                                                        >  3:262024/1‑149 (MQ=255)
                                                    cTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCAtt                                                                                      <  2:177063/148‑1 (MQ=255)
                                                             cAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCct                                                                            >  3:8474/1‑149 (MQ=255)
                                                              aGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCctc                                                                           >  2:285905/1‑149 (MQ=255)
                                                                       cgcTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTc                                                                  <  1:285905/149‑1 (MQ=255)
                                                                             tcgATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCaaaa                                                            <  1:100733/149‑1 (MQ=255)
                                                                             tcgATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCaaaa                                                            >  6:193987/1‑149 (MQ=255)
                                                                               gATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAAtt                                                          >  5:109479/1‑149 (MQ=255)
                                                                                aTCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTg                                                         >  3:215917/1‑149 (MQ=255)
                                                                                   cAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTgcgc                                                      <  4:215917/149‑1 (MQ=255)
                                                                                           gcgcAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGta                                               >  7:155553/1‑148 (MQ=255)
                                                                                           gcgcAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAACTGCGCCGGAGta                                               >  8:122793/1‑148 (MQ=255)
                                                                                                             gTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCcgc                            <  2:297801/149‑1 (MQ=255)
                                                                                                                 aGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAgg                        <  8:296842/149‑1 (MQ=255)
                                                                                                                    ggCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTc                     <  4:8474/149‑1 (MQ=255)
                                                                                                                             tCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATc            >  3:123676/1‑149 (MQ=255)
                                                                                                                               gCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGc          <  2:41057/149‑1 (MQ=255)
                                                                                                                               gCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGc          >  5:183394/1‑149 (MQ=255)
                                                                                                                                 tttCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTg                                 >  6:247862/1‑124 (MQ=255)
                                                                                                                                 tttCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTg                                 <  5:247862/124‑1 (MQ=255)
                                                                                                                                    cgcgGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGt      <  4:123676/148‑1 (MQ=255)
                                                                                                                                     gcgGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGtt     <  6:109479/148‑1 (MQ=255)
                                                                                                                                      cgGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGtttt   <  5:193987/149‑1 (MQ=255)
                                                                                                                                       ggTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGttttt  <  8:155553/149‑1 (MQ=255)
                                                                                                                                       ggTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGttttt  <  4:133813/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                         
GGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTTGCCGGCCAGGCTGACGCGCTCGTCGATCCAGTTGACGCGCAGCGGGTTGATTTCGTGCAGCGGCTTGAACTCGCTTTCGCGGTCCCACCAGCGGTGGGCCAGCGCTTCGAACTTGGCGATTTCGGCGCGGTCGACGTTGCTCATTGAACAGTCCTCTGAAACTTCGCAAAATTGCGCCGGAGTATACCCGAGCGCTTGGCCGCGAGGGTCGCTATAATCGCCGGTTTTT  >  NC_002947/1969321‑1969604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑