Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,010,460 T→A 6.6% E325V (GAA→GTA) ‡ PP_1792 ← group 2 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,010,4600TA 6.6% 183.4 / 2.5 60E325V (GAA→GTA) ‡PP_1792group 2 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (25/31);  new base A (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTGG  >  NC_002947/2010315‑2010601
                                                                                                                                                 |                                                                                                                                             
ttGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCaa                                                                                                                                            >  2:293459/1‑149 (MQ=255)
   tgGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACt                                                                                                                                          <  2:335791/148‑1 (MQ=255)
   tgGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTg                                                                                                                                         >  2:41856/1‑149 (MQ=255)
      cccGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCTCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                      <  3:218151/149‑1 (MQ=255)
      cccGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                      <  7:82917/149‑1 (MQ=255)
      cccGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                      <  5:88725/149‑1 (MQ=255)
      cccGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                      <  8:177145/149‑1 (MQ=255)
       ccGTCGCGGTCACAGTCGGCTTAAGCACAAGCGCATCCTTCCTCCAGCAAATTGCGAAGCTGCTCCATGGCTGGTGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCggg                                                                                                                                     <  5:158340/149‑1 (MQ=255)
       ccGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGAACAACTGCtgg                                                                                                                                     <  2:199919/149‑1 (MQ=255)
          tCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCggg                                                                                                                                     >  5:278959/1‑146 (MQ=255)
          tCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCggg                                                                                                                                     <  6:278959/146‑1 (MQ=255)
             cgGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAg                                                                                                                               <  2:323733/149‑1 (MQ=255)
                    aGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGcc                                                                                                                        >  1:328478/1‑149 (MQ=255)
                    aGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGcc                                                                                                                        >  5:266495/1‑149 (MQ=255)
                      tCGGCATATGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCgg                                                                                                                      <  8:269600/149‑1 (MQ=255)
                        ggCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCgggg                                                                                                                    <  8:24186/149‑1 (MQ=255)
                            tAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGc                                                                                                                <  6:266495/149‑1 (MQ=255)
                             aaGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGcc                                                                                                               >  5:102598/1‑149 (MQ=255)
                               gCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCtt                                                                                                             >  8:119301/1‑149 (MQ=255)
                                cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTc                                                                                                            <  6:132538/149‑1 (MQ=255)
                                cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTc                                                                                                            >  6:113402/1‑149 (MQ=255)
                                cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTc                                                                                                            <  4:165386/149‑1 (MQ=255)
                                cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTc                                                                                                            >  5:304974/1‑149 (MQ=255)
                                  cAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGc                                                                                                          >  4:160793/1‑149 (MQ=255)
                                    aGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCa                                                                                                        <  7:119301/149‑1 (MQ=255)
                                         aaCCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGa                                                                                                   >  6:293885/1‑149 (MQ=255)
                                          aCCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGAt                                                                                                  <  1:41856/149‑1 (MQ=255)
                                          aCCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGAt                                                                                                  <  6:47575/149‑1 (MQ=255)
                                               cttccAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGa                                                                                               <  2:260634/145‑1 (MQ=255)
                                                   cAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACttt                                                                                         >  3:226934/1‑149 (MQ=255)
                                                    aGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACttt                                                                                         >  4:84219/1‑148 (MQ=255)
                                                    aGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTc                                                                                        >  7:283503/1‑149 (MQ=255)
                                                     gCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCggg                                                                                                                                     <  4:280515/103‑1 (MQ=255)
                                                         aTTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGt                                                                                   >  4:121641/1‑149 (MQ=255)
                                                                aGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGcc                                                                            <  6:102598/149‑1 (MQ=255)
                                                                    gcgcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCgg                                                                                                                          >  1:81349/1‑99 (MQ=255)
                                                                    gcgcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCgg                                                                                                                          <  2:81349/99‑1 (MQ=255)
                                                                    gcgcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAgg                                                                        >  7:134086/1‑149 (MQ=255)
                                                                     cgcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCGAg                                                                         <  6:79641/147‑1 (MQ=255)
                                                                       ccATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTa                                                                     <  5:113402/149‑1 (MQ=255)
                                                                            gCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAg                                                                <  3:160793/149‑1 (MQ=255)
                                                                                        aGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTc                                                    >  2:260388/1‑149 (MQ=255)
                                                                                                 ccTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGc                                           <  1:293459/149‑1 (MQ=255)
                                                                                                              ggATAATCCTGTCGCAGCCGGCTGCCAGCAGTTGTTGAACAGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGTACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCaat                               >  4:204922/1‑146 (MQ=255)
                                                                                                               gATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACtt                              <  2:328478/148‑1 (MQ=255)
                                                                                                                    tGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGACCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACt                               <  6:309086/142‑1 (MQ=255)
                                                                                                                    tGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGACCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACt                               >  5:309086/1‑142 (MQ=255)
                                                                                                                     gCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGt                        >  4:235338/1‑148 (MQ=255)
                                                                                                                      cTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTa                       <  8:283503/148‑1 (MQ=255)
                                                                                                                         tCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAg                   >  4:184465/1‑149 (MQ=255)
                                                                                                                           gCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGc                 <  3:184465/149‑1 (MQ=255)
                                                                                                                              gCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCgg              <  3:235338/149‑1 (MQ=255)
                                                                                                                                  gCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTACCAGGCCGGGTCt          >  6:236920/1‑149 (MQ=255)
                                                                                                                                    tccccgcagTTGAACAACTGCTGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTg         >  8:298076/6‑148 (MQ=255)
                                                                                                                                     ccccgcagTTGAACAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGc                                           <  2:217839/109‑1 (MQ=255)
                                                                                                                                     ccccgcagTTGAACAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGc                                           >  1:217839/5‑113 (MQ=255)
                                                                                                                                      ccAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTCGCGTCACCTTCACTTAGCAGTAACACGCCGGGTCTGAAc      >  6:108786/1‑149 (MQ=255)
                                                                                                                                        agcagTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCt    >  6:217550/1‑149 (MQ=255)
                                                                                                                                        agcagTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCt    <  1:260388/149‑1 (MQ=255)
                                                                                                                                          cagTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTgg  <  5:236920/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTGG  >  NC_002947/2010315‑2010601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑