Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,227,422 T→A 7.4% intergenic (‑21/‑426) PP_1964 ← / → holB dGMP/dTMP/dhydroxyCMP kinase/DNA polymerase III subunit delta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,227,4220TA 7.4% 163.5 / 2.5 54intergenic (‑21/‑426)PP_1964/holBdGMP/dTMP/dhydroxyCMP kinase/DNA polymerase III subunit delta'
Reads supporting (aligned to +/- strand):  ref base T (25/25);  new base A (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCAACCTGCTTTGAGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCG  >  NC_002947/2227279‑2227566
                                                                                                                                               |                                                                                                                                                
gCAACCTGCTTTGAGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCgg                                                                                                                                             <  7:237040/149‑1 (MQ=255)
 ctagcTGCTTTGAGAGGATCATCTATGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGAAGGTGTCTTTACGTGATCGAGGTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCAAGGCCCCGAATTTCgg                                                                                                                                             <  4:57644/144‑1 (MQ=255)
  aaCCTGCTTTGAGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCgggg                                                                                                                                           >  4:165045/1‑149 (MQ=255)
     cTGCTTTGAGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGtt                                                                                                                                        <  6:37524/149‑1 (MQ=255)
     cTGCTTTGAGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAAATTCggggcct                                                                                                                                        <  7:133579/149‑4 (MQ=255)
        cTTTGAGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTa                                                                                                                                       <  3:260291/147‑1 (MQ=255)
        cTTTGAGCGGATCAGCTAGGGCATATCGCGCGACCTCAGGGGGGTCCATGAGCAGGGAAGCGAGGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAAATTCggggcctgga                                                                                                                                     <  2:268508/149‑7 (MQ=255)
             aGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGAt                                                                                                                                <  6:184773/149‑1 (MQ=255)
             aGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGAt                                                                                                                                >  5:43457/1‑149 (MQ=255)
              gCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATg                                                                                                                               >  4:316780/1‑149 (MQ=255)
                ggATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATg                                                                                                                               >  2:270115/1‑147 (MQ=255)
                 gATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATg                                                                                                                               >  4:59622/1‑146 (MQ=255)
                    cAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAAc                                                                                                                         >  4:274968/1‑149 (MQ=255)
                        tAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTGGGGGGTTAGAGGGATGTAGAACTCAt                                                                                                                     >  5:74489/1‑149 (MQ=255)
                         aGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATg                                                                                                                    >  2:145065/1‑149 (MQ=255)
                         aGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATg                                                                                                                    >  8:43376/1‑149 (MQ=255)
                          gggCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCAt                                                                                                                     <  3:316780/147‑1 (MQ=255)
                            gCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGttt                                                                                                                 <  3:29513/149‑1 (MQ=255)
                                   tCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCt                                                                                                          >  7:277788/1‑149 (MQ=255)
                                    cccgACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCt                                                                                                          <  7:27845/146‑1 (MQ=255)
                                     gcgACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCtt                                                                                                         <  1:270115/148‑1 (MQ=255)
                                             aGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGaa                                                                                                <  3:59622/149‑1 (MQ=255)
                                              gggTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAAc                                                                                               <  7:43376/149‑1 (MQ=255)
                                                   cTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGtat                                                                                           <  2:48933/148‑1 (MQ=255)
                                                     ccATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTataat                                                                                        >  3:81664/1‑149 (MQ=255)
                                                       aTGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAg                                                                                      >  4:156102/1‑149 (MQ=255)
                                                          aGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGc                                                                                    <  1:145065/148‑1 (MQ=255)
                                                              tGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATg                                                                               <  6:301527/149‑1 (MQ=255)
                                                              tGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATg                                                                               >  7:221627/1‑149 (MQ=255)
                                                                    cGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACATATAATAGGCCTATGTTTACa                                                                         >  3:1633/1‑149 (MQ=255)
                                                                     gACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACAt                                                                        >  6:138413/1‑149 (MQ=255)
                                                                      aCGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACAtt                                                                                                            <  6:192863/112‑1 (MQ=255)
                                                                      aCGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCACGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACAtt                                                                                                            >  5:192863/1‑112 (MQ=255)
                                                                         gtgtCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTTTAATAGGCCTATGTTTACATTTAc                                                                    >  2:191714/1‑149 (MQ=255)
                                                                         gtgtCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTAc                                                                    <  5:138413/149‑1 (MQ=255)
                                                                          tgtCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTAc                                                                    >  3:43649/1‑148 (MQ=255)
                                                                                    ggATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTGGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGaa                                                         <  6:74489/149‑1 (MQ=255)
                                                                                    ggATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGaa                                                         <  6:43457/149‑1 (MQ=255)
                                                                                          aGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATAc                                                      >  2:179589/1‑146 (MQ=255)
                                                                                                  cggcgAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCAcc                                           <  8:221627/149‑1 (MQ=255)
                                                                                                  cggcgAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCAc                                            <  8:277788/148‑1 (MQ=255)
                                                                                                         gCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGcc                                     >  5:219564/1‑148 (MQ=255)
                                                                                                           cGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTAt                                  <  3:165045/149‑1 (MQ=255)
                                                                                                                atgGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAggg                             <  6:229227/149‑1 (MQ=255)
                                                                                                                atgGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAgg                              <  4:43649/148‑1 (MQ=255)
                                                                                                                        cATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGt                      <  3:274968/148‑1 (MQ=255)
                                                                                                                         aTTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCg                    >  2:68473/1‑149 (MQ=255)
                                                                                                                         aTTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCg                    >  2:206022/1‑149 (MQ=255)
                                                                                                                         aTTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCg                    >  5:178888/1‑149 (MQ=255)
                                                                                                                                 ctctaacccccGAAATTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTgt            >  7:65488/8‑149 (MQ=255)
                                                                                                                                     aacccccGAAATTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTgtcggt        >  8:121715/4‑149 (MQ=255)
                                                                                                                                        ccccGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTgtcggtc       >  6:346198/1‑147 (MQ=255)
                                                                                                                                        ccccGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGa     <  4:81664/149‑1 (MQ=255)
                                                                                                                                           cGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCg  >  6:168792/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GCAACCTGCTTTGAGCGGATCAGCTAGGGCATATGTCGCGACCTCAGGGTGCTCCATGAGCATGGAAGCGACGGTGTCTTTACCGGATCGAGCTACTGCGGCGAGGCCGATGATGGTTGGCATTTTTGGCTCCAGGCCCCGAATTTCGGGGGTTAGAGGGATGTAGAACTCATGTTTACATTCTTAGTAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCG  >  NC_002947/2227279‑2227566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑