Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,238,142 A→T 8.0% F148I (TTC→ATC)  PP_1975 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,238,1420AT 8.0% 143.3 / 2.3 50F148I (TTC→ATC) PP_1975EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base A (20/26);  new base T (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TTCTTCTCAAGCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAGCACAG  >  NC_002947/2237994‑2238286
                                                                                                                                                    |                                                                                                                                                
ttcttcTCAAGCCCGTATAACAAGCCGGCGATCATTAGCAGGACCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCAAGTTCTCGGTCAGCCAGCAGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGAGGTCATGGCGaa                                                                                                                                                  >  2:111047/1‑149 (MQ=255)
       cAAGCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGc                                                                                                                                            >  7:25718/1‑148 (MQ=255)
          gCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATg                                                                                                                                        >  6:97649/1‑149 (MQ=255)
          gCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATg                                                                                                                                        >  2:200155/1‑149 (MQ=255)
           cccGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGATGTTCGCCATga                                                                                                                                       <  7:63425/149‑2 (MQ=255)
            ccGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGATGTTCGCCATga                                                                                                                                       >  8:63425/1‑147 (MQ=255)
            ccGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGACCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGcc                                                                                                                                      >  7:209984/1‑149 (MQ=255)
                      aGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCgg                                                                                                                            >  5:199323/1‑149 (MQ=255)
                        ccGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCggtg                                                                                                                          <  6:44175/149‑1 (MQ=255)
                        ccGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCggtg                                                                                                                          <  2:80609/149‑1 (MQ=255)
                              aTCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGATCATCGCCATGCCTTTGGCGCGGTGGTGCTc                                                                                                                    <  1:27611/149‑1 (MQ=255)
                                  ttAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCggg                                                                                                                 >  1:306665/1‑148 (MQ=255)
                                        ggCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTg                                                                                                          <  2:174504/149‑1 (MQ=255)
                                         gaaaggTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCCGTCAGACAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCCGTGGTGATCGGGCAGCTTGa                                                                                                         >  2:341312/5‑149 (MQ=255)
                                           ccGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGAt                                                                                                        >  5:78936/1‑148 (MQ=255)
                                              gTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGc                                                                                                    <  1:100660/149‑1 (MQ=255)
                                                            tAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTTGGTCCGATGGAGGGTGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAgg                                                                                       <  6:341827/148‑1 (MQ=255)
                                                            tAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAgg                                                                                       <  2:219529/148‑1 (MQ=255)
                                                              gaagaTGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGc                                                                                      >  5:294843/1‑147 (MQ=255)
                                                                 gaTGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCg                                                                                 <  1:200155/149‑1 (MQ=255)
                                                                 gaTGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCg                                                                                 <  3:43963/149‑1 (MQ=255)
                                                                  aTGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCgg                                                                                <  5:330132/149‑1 (MQ=255)
                                                                       ttCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGAt                                                                             >  6:217445/1‑147 (MQ=255)
                                                                          ccAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAgcg                                                                        >  3:106398/1‑149 (MQ=255)
                                                                           cAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAgcgc                                                                       <  2:306665/149‑1 (MQ=255)
                                                                             gCCCCAGTTCTCGGTCAGCCAGGCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAgcgcgc                                                                     <  6:339850/149‑1 (MQ=255)
                                                                               cccAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTg                                                                                           >  4:199966/1‑125 (MQ=255)
                                                                               cccAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTg                                                                                           <  3:199966/125‑1 (MQ=255)
                                                                               cccAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCa                                                                   >  2:8728/1‑149 (MQ=255)
                                                                                ccAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCAc                                                                  >  1:34324/1‑149 (MQ=255)
                                                                                    ttCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGt                                                              >  5:81109/1‑149 (MQ=255)
                                                                                      ctcGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTg                                                             <  6:199323/148‑1 (MQ=255)
                                                                                         ggTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGaaa                                                          <  6:294843/148‑1 (MQ=255)
                                                                                         ggTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAAc                                                         <  5:97649/149‑1 (MQ=255)
                                                                                                cagccGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTTCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCaa                                                  <  8:209984/149‑1 (MQ=255)
                                                                                                 agccGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAg                                                 >  3:302770/1‑149 (MQ=255)
                                                                                                    cgccCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCa                                              <  6:78936/149‑1 (MQ=255)
                                                                                                     gccCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCAc                                             >  4:87393/1‑149 (MQ=255)
                                                                                                      cccAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACg                                            >  6:185351/1‑149 (MQ=255)
                                                                                                               ggtCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGa                                    <  3:87393/148‑1 (MQ=255)
                                                                                                                gtCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATc                                  <  1:295013/149‑1 (MQ=255)
                                                                                                                   cGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGTTGAAACCCTGCAAGGCACGGAACAGGATCATg                               <  5:185351/149‑1 (MQ=255)
                                                                                                                    gATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATg                               >  8:120538/1‑148 (MQ=255)
                                                                                                                         aGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGTTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCAAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGTAACAGGATCATGCTCTcc                         <  1:341312/149‑1 (MQ=255)
                                                                                                                             ggCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGt                     <  5:217445/149‑1 (MQ=255)
                                                                                                                               cAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTc                   <  6:81109/149‑1 (MQ=255)
                                                                                                                                  aaGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTTATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCGACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAg                <  7:120538/149‑1 (MQ=255)
                                                                                                                                  aaGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAg                <  4:302770/149‑1 (MQ=255)
                                                                                                                                        cGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATc          <  1:8728/149‑1 (MQ=255)
                                                                                                                                           gcATGGCGATGTTCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAg       >  4:206682/2‑149 (MQ=255)
                                                                                                                                                gCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAGCAcag  >  2:6425/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TTCTTCTCAAGCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAGCACAG  >  NC_002947/2237994‑2238286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑