| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,572 | G→A | 13.7% | K1026K (AAG→AAA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,572 | 0 | G | A | 13.7% | 261.4 / 27.3 | 109 | K1026K (AAG→AAA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (50/44); new base A (5/10); total (55/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
GATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACT > NC_002947/197426‑197720 | caccacCGCGACCCTGACGGCGGAAACGTCGGACACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCAAGACGCCGGTAACCGTGACCCAGACCAACGGCCAAACAAATACCGTAGA‑AGCCGCCAAAACCCAgg > 7:51326/1‑149 (MQ=11) caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAgg > 6:93250/1‑149 (MQ=37) caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAgg > 7:78617/1‑149 (MQ=37) caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAgg < 1:43346/149‑1 (MQ=37) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAggg > 1:163981/1‑149 (MQ=37) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAggg > 5:19314/1‑149 (MQ=37) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAggg < 8:312271/149‑1 (MQ=37) caccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCa < 1:22668/149‑1 (MQ=37) cgcgACCCTGACGGCGCGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCg > 5:67015/1‑149 (MQ=37) cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAGCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCg > 1:277864/1‑149 (MQ=37) cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGAt < 2:193742/149‑1 (MQ=37) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGAtt > 1:152276/1‑149 (MQ=37) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGAtt > 1:157395/1‑149 (MQ=37) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTcc < 7:293100/148‑1 (MQ=21) gAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGAccc > 1:137927/1‑149 (MQ=37) ccGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGAt > 8:142128/1‑135 (MQ=37) ccGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGAt < 7:142128/135‑1 (MQ=37) ggTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt > 8:300789/1‑148 (MQ=37) tCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GAc < 2:300175/149‑1 (MQ=37) tCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GAc < 5:298649/149‑1 (MQ=37) cGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTc > 5:180369/1‑148 (MQ=37) cGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTc > 3:63666/1‑148 (MQ=37) cGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACACTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCAAGGGCAGCGACGATTTCCAGACCCCGGCGAAA‑‑GACGTc > 2:219168/1‑148 (MQ=255) aaGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTa > 1:159055/1‑148 (MQ=37) cgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACg < 2:159055/149‑1 (MQ=37) gATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGtt > 2:174044/1‑149 (MQ=37) aCCTACCCCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa < 2:157395/148‑1 (MQ=37) aCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa < 2:152276/148‑1 (MQ=37) aCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa < 1:174044/148‑1 (MQ=37) aCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa < 6:19314/148‑1 (MQ=37) cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGAccg > 2:12162/1‑147 (MQ=25) cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTg > 5:55619/1‑149 (MQ=38) acacCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGAtt < 1:211760/105‑1 (MQ=40) cacCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCa < 7:300789/149‑1 (MQ=39) cGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCg > 1:69086/1‑149 (MQ=39) cGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCg > 5:187322/1‑149 (MQ=21) aTCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCa < 3:159506/148‑1 (MQ=18) ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCa > 8:69459/1‑149 (MQ=37) ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCa > 5:115947/1‑149 (MQ=17) cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCa > 6:339914/1‑148 (MQ=17) tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc < 2:69086/149‑1 (MQ=37) tGAGCAATCCTGCCCAGACGCCGGAAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc > 5:231775/1‑149 (MQ=37) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTTTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCga < 6:315254/149‑1 (MQ=17) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCTATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc < 4:142246/147‑1 (MQ=37) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGTCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc > 8:68717/1‑147 (MQ=37) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCga < 3:237283/149‑1 (MQ=17) aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCAAGGCCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCCTCAGCGTCACCATc > 1:128849/1‑147 (MQ=255) cAATCCTGCCCAGACGCCGGTAACCGTGACGCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑g > 1:29042/1‑107 (MQ=25) cAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑g < 2:29042/107‑1 (MQ=35) cAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgag > 3:301354/1‑148 (MQ=37) tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAAc > 4:68781/1‑148 (MQ=37) tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAAc < 3:68781/148‑1 (MQ=37) ccTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAACCCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGc > 3:209135/1‑132 (MQ=37) tGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCca > 2:307518/1‑149 (MQ=37) ccAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCAATACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAg > 5:99165/1‑149 (MQ=17) aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGc < 6:115947/149‑1 (MQ=17) aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGc < 7:270043/149‑1 (MQ=17) aCGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGA‑AGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCgg > 8:103008/1‑149 (MQ=255) aCGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCgg > 2:320380/1‑149 (MQ=17) gCCGTTGACCGTGACCCTGTCCAACGTCCAAACGATTACCGTTTA‑AGCCGGCTAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTAGAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTa < 5:13716/149‑1 (MQ=11) gTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAttt < 6:187322/149‑1 (MQ=17) tgaCCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGA‑AGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTc < 4:207648/147‑1 (MQ=255) aCCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 2:277864/149‑1 (MQ=34) aCCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 6:67015/149‑1 (MQ=34) aCCGTGACCCTGACCAACGGCCAAACGATTACCGTTGA‑AGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 7:199823/149‑1 (MQ=255) ccGTGACCCTGTCCAACGGCCAAACGATTACCGTTGA‑AGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg < 4:127656/149‑1 (MQ=16) cGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg > 1:227537/1‑148 (MQ=32) cGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg > 4:313807/1‑148 (MQ=32) cGTGACCCTCTCCAACGGCCAAACTCTTAGCGTTGA‑AGCCGCCAAAACACAGGGCAGCGTCGACTTCCAGACCCCGGCGAATTCCTCGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 1:272302/149‑1 (MQ=11) gTGACCCTGTCCAACGGCCAAACCATTACCGTTGAGAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg > 1:50557/1‑148 (MQ=17) ccTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGCCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa > 2:211919/1‑149 (MQ=17) ccTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTg > 4:48493/1‑148 (MQ=32) ccTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa > 3:63171/1‑149 (MQ=32) ccTGTCAAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa > 1:87079/1‑149 (MQ=17) cTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgaga > 3:195566/1‑118 (MQ=32) cTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgaga < 4:195566/118‑1 (MQ=32) cAACGGCCAAACGATTACCGTTGA‑AGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCg > 3:138846/1‑148 (MQ=12) aaCGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCTAGCAACTGACCCCGaa < 6:55619/149‑1 (MQ=17) aaCGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaa > 8:227749/1‑149 (MQ=32) aaaCCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACg > 7:198715/1‑149 (MQ=35) aCCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGcc < 2:50557/149‑1 (MQ=37) gaTTACCGTTGA‑AGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCgg < 2:173990/148‑1 (MQ=12) cATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCgg < 7:68717/149‑1 (MQ=37) ttACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt > 4:247854/1‑149 (MQ=37) cGTTGA‑AGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGa < 4:138846/149‑1 (MQ=12) ttGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt < 8:72905/143‑1 (MQ=37) ttGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt > 7:72905/1‑143 (MQ=37) gA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAc > 2:153834/1‑149 (MQ=37) a‑aGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGAccgc < 1:307518/148‑3 (MQ=37) a‑aGCCGGCAAAACCCAGTGCAGCGTCGACTTCCAGACCCCTGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACg < 1:210653/149‑1 (MQ=11) ccGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGAt > 7:22579/1‑148 (MQ=37) cGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATc > 7:128362/1‑148 (MQ=12) ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAc < 3:313807/149‑1 (MQ=38) ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAc < 4:63666/149‑1 (MQ=38) ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATCTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGAc < 6:295370/140‑1 (MQ=255) ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATCTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACC‑CGATcgg > 5:295370/1‑146 (MQ=11) aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTaa < 2:148507/98‑1 (MQ=33) aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTaa > 1:148507/1‑98 (MQ=33) aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGa < 2:137927/149‑1 (MQ=39) gACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACt < 2:227537/149‑1 (MQ=39) | GATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGA‑AGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACT > NC_002947/197426‑197720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |