Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,265,671 T→A 9.6% L244Q (CTG→CAG)  folC → bifunctional folypolyglutamate synthase/dihydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,265,6710TA 9.6% 143.0 / 5.9 52L244Q (CTG→CAG) folCbifunctional folypolyglutamate synthase/dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base T (18/29);  new base A (3/2);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

GTGATCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTAAT  >  NC_002947/2265524‑2265819
                                                                                                                                                   |                                                                                                                                                    
gTGATCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTg                                                                                                                                                     <  8:250782/149‑1 (MQ=255)
   aTCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTgcgc                                                                                                                                                  >  4:20651/1‑149 (MQ=255)
    tCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTgcgc                                                                                                                                                  <  8:313203/148‑1 (MQ=255)
      tGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGAc                                                                                                                                               >  7:96233/1‑149 (MQ=255)
        gATCCGCCCGGGCCGTTGCTGGACACGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGa                                                                                                                                                <  5:197896/146‑1 (MQ=255)
        gATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCt                                                                                                                                             <  1:170549/149‑1 (MQ=255)
         aTCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGCGCAGCTCGACCTg                                                                                                                                            <  2:86052/149‑1 (MQ=255)
         aTCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTg                                                                                                                                            >  1:68009/1‑149 (MQ=255)
         aTCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTg                                                                                                                                            <  7:194731/149‑1 (MQ=255)
           ccGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACCGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGcc                                                                                                                                          >  4:55288/1‑149 (MQ=255)
             gCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGCGCAGCTCGACCTGcgca                                                                                                                                        <  6:313502/149‑4 (MQ=255)
                          cTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGc                                                                                                                            <  1:136881/148‑1 (MQ=255)
                             gACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGccc                                                                                                                          <  4:127380/147‑1 (MQ=255)
                             gACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCAt                                                                                                                        >  3:29211/1‑149 (MQ=255)
                               cAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATg                                                                                                                       >  1:246297/1‑148 (MQ=255)
                                    ccgccgAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAAc                                                                                                                 <  2:68009/149‑1 (MQ=255)
                                     cgccgAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACg                                                                                                                >  4:124711/1‑149 (MQ=255)
                                     cgccgAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACg                                                                                                                >  4:262508/1‑149 (MQ=255)
                                               gCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTgg                                                                                                       <  8:96233/148‑1 (MQ=255)
                                                    gcCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGc                                                                                                 <  2:245018/149‑1 (MQ=255)
                                                       aCTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAgg                                                                                              <  2:224599/149‑1 (MQ=255)
                                                       aCTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGAGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAgg                                                                                              >  5:241226/1‑149 (MQ=255)
                                                         tGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCt                                                                                            >  5:223239/1‑149 (MQ=255)
                                                           ttCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCttt                                                                                          <  1:6861/149‑1 (MQ=255)
                                                            tCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCt                                                                                                         >  5:281467/1‑133 (MQ=255)
                                                            tCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCt                                                                                                         <  6:281467/133‑1 (MQ=255)
                                                            tCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTc                                                                                         >  7:219245/1‑149 (MQ=255)
                                                              cTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCGTTCct                                                                                       >  8:214085/1‑149 (MQ=255)
                                                               tGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCctg                                                                                      >  7:97829/1‑149 (MQ=255)
                                                                 cgcgGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCctg                                                                                      <  7:148513/147‑1 (MQ=255)
                                                                      tCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATgg                                                                               <  3:20651/149‑1 (MQ=255)
                                                                        gggAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATgggg                                                                             <  8:97829/149‑1 (MQ=255)
                                                                             ttCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGcc                                                                                                     >  5:58136/1‑120 (MQ=255)
                                                                             ttCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGcc                                                                                                     <  6:58136/120‑1 (MQ=255)
                                                                                caCCGGGCCAGGGCCGAAGGCTGCTGGAGCTGGCGGGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCGTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTg                                                                     <  3:169182/148‑1 (MQ=255)
                                                                                        cAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACgcgg                                                             <  3:262508/149‑1 (MQ=255)
                                                                                             ccGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCaa                                                        <  6:241226/149‑1 (MQ=255)
                                                                                                    ctgctgGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCTCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGcc                                                 <  8:59690/149‑1 (MQ=255)
                                                                                                       ctgGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAg                                               <  4:282044/148‑1 (MQ=255)
                                                                                                        tgGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgc                                             >  4:252021/1‑149 (MQ=255)
                                                                                                          gAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgcgc                                           >  6:29479/1‑149 (MQ=255)
                                                                                                          gAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgcgc                                           <  5:29479/149‑1 (MQ=255)
                                                                                                            gCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCACCAGGCgctg                                         <  4:29211/149‑1 (MQ=255)
                                                                                                                 cGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGa                                    <  2:246297/149‑1 (MQ=255)
                                                                                                                     gTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATAc                                 <  8:219245/148‑1 (MQ=255)
                                                                                                                          gCCGCTGATCGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTCCCCATGGAAAACGCCACGCTGGCCTTGCAGGCATTCCTGCTGATGGGGCTGCCGAGGAACGCGGCGCAAGTCCGCCAGGAGCTGCAGGATACCCGTAt                           >  2:256690/1‑149 (MQ=255)
                                                                                                                           ccGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCGACGCTGGCCTTGCAGGCTTTCGTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTAt                           <  1:256690/148‑1 (MQ=255)
                                                                                                                                  tGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGc                   <  3:55288/149‑1 (MQ=255)
                                                                                                                                  gcaaaggcAGGTCGCGCAGCTCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgc                                             >  2:62554/7‑123 (MQ=255)
                                                                                                                                     aaggcAGGTCGCGCAGCTCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGtc                >  1:214745/4‑149 (MQ=255)
                                                                                                                                      aggcAGGTCGCGCAGCTCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGtct               >  7:221969/3‑149 (MQ=255)
                                                                                                                                       cgcAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGtctc              <  3:124711/149‑1 (MQ=255)
                                                                                                                                                   tGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTAAt  >  8:228710/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTGATCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTAAT  >  NC_002947/2265524‑2265819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑