Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,265,674 G→T 10.0% R245L (CGC→CTC)  folC → bifunctional folypolyglutamate synthase/dihydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,265,6740GT10.0% 146.1 / 7.2 50R245L (CGC→CTC) folCbifunctional folypolyglutamate synthase/dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base G (18/27);  new base T (3/2);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

ATCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTAATGT  >  NC_002947/2265527‑2265821
                                                                                                                                                   |                                                                                                                                                   
aTCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTgcgc                                                                                                                                                    >  4:20651/1‑149 (MQ=255)
 tCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTgcgc                                                                                                                                                    <  8:313203/148‑1 (MQ=255)
   tGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGAc                                                                                                                                                 >  7:96233/1‑149 (MQ=255)
     gATCCGCCCGGGCCGTTGCTGGACACGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGa                                                                                                                                                  <  5:197896/146‑1 (MQ=255)
     gATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCt                                                                                                                                               <  1:170549/149‑1 (MQ=255)
      aTCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGCGCAGCTCGACCTg                                                                                                                                              <  2:86052/149‑1 (MQ=255)
      aTCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTg                                                                                                                                              <  7:194731/149‑1 (MQ=255)
      aTCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTg                                                                                                                                              >  1:68009/1‑149 (MQ=255)
        ccGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACCGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGcc                                                                                                                                            >  4:55288/1‑149 (MQ=255)
          gCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGCGCAGCTCGACCTGcgca                                                                                                                                          <  6:313502/149‑4 (MQ=255)
                       cTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGc                                                                                                                              <  1:136881/148‑1 (MQ=255)
                          gACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGccc                                                                                                                            <  4:127380/147‑1 (MQ=255)
                          gACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCAt                                                                                                                          >  3:29211/1‑149 (MQ=255)
                            cAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATg                                                                                                                         >  1:246297/1‑148 (MQ=255)
                                 ccgccgAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAAc                                                                                                                   <  2:68009/149‑1 (MQ=255)
                                  cgccgAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACg                                                                                                                  >  4:262508/1‑149 (MQ=255)
                                  cgccgAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACg                                                                                                                  >  4:124711/1‑149 (MQ=255)
                                            gCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTgg                                                                                                         <  8:96233/148‑1 (MQ=255)
                                                 gcCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGc                                                                                                   <  2:245018/149‑1 (MQ=255)
                                                    aCTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAgg                                                                                                <  2:224599/149‑1 (MQ=255)
                                                    aCTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGAGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAgg                                                                                                >  5:241226/1‑149 (MQ=255)
                                                      tGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCt                                                                                              >  5:223239/1‑149 (MQ=255)
                                                        ttCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCttt                                                                                            <  1:6861/149‑1 (MQ=255)
                                                         tCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCt                                                                                                           <  6:281467/133‑1 (MQ=255)
                                                         tCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCt                                                                                                           >  5:281467/1‑133 (MQ=255)
                                                         tCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTc                                                                                           >  7:219245/1‑149 (MQ=255)
                                                           cTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCGTTCct                                                                                         >  8:214085/1‑149 (MQ=255)
                                                            tGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCctg                                                                                        >  7:97829/1‑149 (MQ=255)
                                                              cgcgGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCctg                                                                                        <  7:148513/147‑1 (MQ=255)
                                                                   tCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATgg                                                                                 <  3:20651/149‑1 (MQ=255)
                                                                     gggAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATgggg                                                                               <  8:97829/149‑1 (MQ=255)
                                                                          ttCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGcc                                                                                                       >  5:58136/1‑120 (MQ=255)
                                                                          ttCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGcc                                                                                                       <  6:58136/120‑1 (MQ=255)
                                                                             caCCGGGCCAGGGCCGAAGGCTGCTGGAGCTGGCGGGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCGTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTg                                                                       <  3:169182/148‑1 (MQ=255)
                                                                                     cAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACgcgg                                                               <  3:262508/149‑1 (MQ=255)
                                                                                          ccGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCaa                                                          <  6:241226/149‑1 (MQ=255)
                                                                                                 ctgctgGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCTCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGcc                                                   <  8:59690/149‑1 (MQ=255)
                                                                                                    ctgGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAg                                                 <  4:282044/148‑1 (MQ=255)
                                                                                                     tgGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgc                                               >  4:252021/1‑149 (MQ=255)
                                                                                                       gAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgcgc                                             <  5:29479/149‑1 (MQ=255)
                                                                                                       gAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgcgc                                             >  6:29479/1‑149 (MQ=255)
                                                                                                         gCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCACCAGGCgctg                                           <  4:29211/149‑1 (MQ=255)
                                                                                                              cGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGa                                      <  2:246297/149‑1 (MQ=255)
                                                                                                                  gTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATAc                                   <  8:219245/148‑1 (MQ=255)
                                                                                                                       gCCGCTGATCGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTCCCCATGGAAAACGCCACGCTGGCCTTGCAGGCATTCCTGCTGATGGGGCTGCCGAGGAACGCGGCGCAAGTCCGCCAGGAGCTGCAGGATACCCGTAt                             >  2:256690/1‑149 (MQ=255)
                                                                                                                        ccGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCGACGCTGGCCTTGCAGGCTTTCGTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTAt                             <  1:256690/148‑1 (MQ=255)
                                                                                                                               tGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGc                     <  3:55288/149‑1 (MQ=255)
                                                                                                                               gcaaaggcAGGTCGCGCAGCTCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGgc                                               >  2:62554/7‑123 (MQ=255)
                                                                                                                                  aaggcAGGTCGCGCAGCTCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGtc                  >  1:214745/4‑149 (MQ=255)
                                                                                                                                   aggcAGGTCGCGCAGCTCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGtct                 >  7:221969/3‑149 (MQ=255)
                                                                                                                                    cgcAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGtctc                <  3:124711/149‑1 (MQ=255)
                                                                                                                                                tGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTAAt    >  8:228710/1‑149 (MQ=255)
                                                                                                                                                  cgcgACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTAAtgt  <  3:252021/149‑1 (MQ=255)
                                                                                                                                                   gcgACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTaa     <  1:182484/145‑1 (MQ=255)
                                                                                                                                                   gcgACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTaa     >  2:182484/1‑145 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCTGGATCCGCCCGGGCCGTTGCTGGACAAGGCCGCCGAACTGGCCGCGCCACTGTTCCTGCGCGGTCGGGAATTCGACCTGGCCAGTGCCGAAGGCTGCTGGAGCTGGCGTGGTACTGCCGCTGATGGTGCGCAGGTCGAGCTGCGCGACCTGCCTTTGCTGGACCTGCCCATGGAAAACGCCACGCTGGCCTTGCAGGCTTTCCTGCTGATGGGGCTGCCGTGGAACGCGGCGCAAGTCCGCCAGGCGCTGCTGGATACCCGTATCACTGGGCGTCTCGACCTCCGTAATGT  >  NC_002947/2265527‑2265821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑