Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,273,062 C→G 14.9% intergenic (+27/+264) PP_2003 → / ← PP_2004 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,273,0620CG14.9% 139.6 / 8.4 51intergenic (+27/+264)PP_2003/PP_2004hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (19/24);  new base G (4/4);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGGCGCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGA  >  NC_002947/2272914‑2273203
                                                                                                                                                    |                                                                                                                                              
cAGCGGCGCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGcc                                                                                                                                                >  8:147925/1‑149 (MQ=255)
cAGCGGCGCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGcc                                                                                                                                                >  8:129729/1‑149 (MQ=255)
  gcggcgCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCgg                                                                                                                                              <  2:120657/149‑1 (MQ=255)
              gtggATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGaaa                                                                                                                                  <  1:160853/149‑1 (MQ=255)
                 gATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCGGGCTGCCGGCGAAAggg                                                                                                                               <  3:116620/149‑1 (MQ=255)
                 gATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAgg                                                                                                                                <  2:339705/148‑1 (MQ=255)
                     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg                                                                                                                            <  6:248963/148‑1 (MQ=39)
                     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCg                                                                                                                            <  6:301691/148‑1 (MQ=255)
                     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTAGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg                                                                                                                            <  3:67483/148‑1 (MQ=38)
                      cAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGt                                                                                                                          <  7:147925/149‑1 (MQ=255)
                       aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGtt                                                                                                                         <  7:129729/149‑1 (MQ=255)
                         ccAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCa                                                                                                                       <  7:1066/149‑1 (MQ=255)
                              gTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTg                                                                                                                  >  8:59202/1‑149 (MQ=255)
                                   cAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAAc                                                                                                             <  5:128421/149‑1 (MQ=255)
                                         gtgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCCACCCATTCGCCGGCCGCCACCGAAATGGCCAGTTAAGGCTGAAAACAAccc                                                                                                        >  6:147971/1‑148 (MQ=255)
                                                cgccgGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACa                                                                                                <  2:236058/149‑1 (MQ=255)
                                                cgccgGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCGCCCTTTGATGGGTCGCCTGAACCCCCCCTTTCGCCGGCCGCCCGCGAAAGGGCCCGGTCACGCTGTAAACCTccccgggta                                                                                                  >  5:152831/1‑142 (MQ=255)
                                                   cggccgAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGAc                                                                                             <  7:239678/149‑1 (MQ=255)
                                                                aCCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAAc                                                                               >  5:111292/1‑149 (MQ=255)
                                                                 cccATTCCGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTAGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCAGTAAACATCCCCTGTTACAGACGCCCCGCG‑AAAACt                                                                              >  2:84496/1‑149 (MQ=255)
                                                                    aTTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTc                                                                           <  7:29679/149‑1 (MQ=255)
                                                                    aTTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCAGAACCCACCCTTTCGCCGGCAGCCCGCCAAAGGGCCGGTTCAGGCTGTAAAAAACCCCAGTTAAAGACGCCCCGCC‑AATACttta                                                                           >  4:227614/1‑148 (MQ=255)
                                                                     ttCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCt                                                                          <  3:227614/149‑1 (MQ=255)
                                                                            gcagcaGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCGTTCGCCGGCAGCCCGCGAAAGGGGCGGGTCAGGCTGTTAACATACCCGGTTTCAGACGCCCCGCG‑ATAACTTTTTGCAAAcc                                                                   >  7:121902/1‑149 (MQ=255)
                                                                                  ggATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTAc                                                             <  7:59202/149‑1 (MQ=255)
                                                                                    aTGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTAGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATACCCTGTTACAGACGCCCCGCG‑ATAACTTTCTg                                                                         >  8:172404/1‑135 (MQ=255)
                                                                                        ccAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCAGAACCGACCCATTAGCCGGCCGCCCGCGAAACGGCCGGTTAAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCAGAACCCCTGGAACGTTTaa                                                       >  6:138164/1‑148 (MQ=255)
                                                                                         cAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGCAAGGGCCGGTTCAGGCTGGAAACATCCCCTGTTACAGCCGCCCCGCG‑ATAACTTTGTGAACACCTGCTACGTTTAc                                                       >  5:264650/1‑148 (MQ=255)
                                                                                                      ccgccgccCCCTCCATTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCTAAAGGGCCGGTTCAGGCTGAAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCtt                                          <  5:147971/148‑1 (MQ=255)
                                                                                                       cgccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGt                                        >  5:72372/1‑149 (MQ=255)
                                                                                                        gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGt                                        >  6:168447/1‑148 (MQ=255)
                                                                                                        gccgccCCCTCCCTTTGATGGGTCGCCGGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtc                                       <  6:152831/149‑1 (MQ=255)
                                                                                                            cccccTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtcatca                                   <  5:168447/149‑1 (MQ=255)
                                                                                                              ccctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGCCGCCCCGCG‑ATAACGTTCTGCACACCTGGTACGTTTACCACTGAGAAGCTTGGCataatc                                 >  7:143160/1‑149 (MQ=255)
                                                                                                              ccctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGCATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTcatcat                                  <  1:84496/149‑1 (MQ=255)
                                                                                                               cctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCGGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATcc                                >  7:163675/1‑149 (MQ=255)
                                                                                                                ctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCAt                                                <  2:234147/132‑1 (MQ=255)
                                                                                                                         aTGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCcg                                                                                       <  3:79745/85‑1 (MQ=255)
                                                                                                                            ggTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCTGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCt                   <  8:163675/149‑1 (MQ=255)
                                                                                                                             gTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTg                  <  6:72372/149‑1 (MQ=255)
                                                                                                                              tCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt                              >  7:137647/1‑136 (MQ=255)
                                                                                                                              tCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt                              <  8:137647/136‑1 (MQ=255)
                                                                                                                                 ccTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCt                >  4:164544/1‑147 (MQ=255)
                                                                                                                                  cTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACCCCTGCTACGTTTACCAGTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg             >  3:275780/1‑149 (MQ=255)
                                                                                                                                           cccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtc                                       <  7:121816/114‑1 (MQ=255)
                                                                                                                                           cccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtc                                       >  8:121816/1‑114 (MQ=255)
                                                                                                                                            ccTTTCGCGGGCTGCCGGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACCCCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGc    >  6:247900/1‑148 (MQ=255)
                                                                                                                                            ccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc                                            >  6:30245/1‑108 (MQ=255)
                                                                                                                                            ccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc                                            <  5:30245/108‑1 (MQ=255)
                                                                                                                                             cTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGCCAGTAAACATCCCATGTTACAGACGCCACGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCAATCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCg   >  6:53286/1‑148 (MQ=255)
                                                                                                                                             cTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa  >  3:80901/1‑149 (MQ=255)
                                                                                                                                              tttCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg             <  3:228163/137‑1 (MQ=255)
                                                                                                                                              tttCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg             >  4:228163/1‑137 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CAGCGGCGCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGA  >  NC_002947/2272914‑2273203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑