| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,273,066 | A→T | 15.9% | intergenic (+31/+260) | PP_2003 → / ← PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,273,066 | 0 | A | T | 15.9% | 100.8 / 12.9 | 50 | intergenic (+31/+260) | PP_2003/PP_2004 | hypothetical protein/AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (16/23); new base T (4/4); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01 | |||||||||||
GTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGA > NC_002947/2272928‑2273203 | gtggATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGaaa < 1:160853/149‑1 (MQ=255) gATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCGGGCTGCCGGCGAAAggg < 3:116620/149‑1 (MQ=255) gATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAgg < 2:339705/148‑1 (MQ=255) aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg < 6:248963/148‑1 (MQ=39) aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCg < 6:301691/148‑1 (MQ=255) aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTAGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg < 3:67483/148‑1 (MQ=38) cAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGt < 7:147925/149‑1 (MQ=255) aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGtt < 7:129729/149‑1 (MQ=255) ccAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCa < 7:1066/149‑1 (MQ=255) gTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTg > 8:59202/1‑149 (MQ=255) cAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAAc < 5:128421/149‑1 (MQ=255) gtgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCCACCCATTCGCCGGCCGCCACCGAAATGGCCAGTTAAGGCTGAAAACAAccc > 6:147971/1‑148 (MQ=255) cgccgGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACa < 2:236058/149‑1 (MQ=255) cgccgGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCGCCCTTTGATGGGTCGCCTGAACCCCCCCTTTCGCCGGCCGCCCGCGAAAGGGCCCGGTCACGCTGTAAACCTccccgggta > 5:152831/1‑142 (MQ=255) cggccgAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGAc < 7:239678/149‑1 (MQ=255) aCCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAAc > 5:111292/1‑149 (MQ=255) cccATTCCGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTAGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCAGTAAACATCCCCTGTTACAGACGCCCCGCG‑AAAACt > 2:84496/1‑149 (MQ=255) aTTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTc < 7:29679/149‑1 (MQ=255) aTTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCAGAACCCACCCTTTCGCCGGCAGCCCGCCAAAGGGCCGGTTCAGGCTGTAAAAAACCCCAGTTAAAGACGCCCCGCC‑AATACttta > 4:227614/1‑148 (MQ=255) ttCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCt < 3:227614/149‑1 (MQ=255) gcagcaGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCGTTCGCCGGCAGCCCGCGAAAGGGGCGGGTCAGGCTGTTAACATACCCGGTTTCAGACGCCCCGCG‑ATAACTTTTTGCAAAcc > 7:121902/1‑149 (MQ=255) ggATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTAc < 7:59202/149‑1 (MQ=255) aTGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTAGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATACCCTGTTACAGACGCCCCGCG‑ATAACTTTCTg > 8:172404/1‑135 (MQ=255) ccAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCAGAACCGACCCATTAGCCGGCCGCCCGCGAAACGGCCGGTTAAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCAGAACCCCTGGAACGTTTaa > 6:138164/1‑148 (MQ=255) cAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGCAAGGGCCGGTTCAGGCTGGAAACATCCCCTGTTACAGCCGCCCCGCG‑ATAACTTTGTGAACACCTGCTACGTTTAc > 5:264650/1‑148 (MQ=255) ccgccgccCCCTCCATTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCTAAAGGGCCGGTTCAGGCTGAAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCtt < 5:147971/148‑1 (MQ=255) cgccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGt > 5:72372/1‑149 (MQ=255) gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGt > 6:168447/1‑148 (MQ=255) gccgccCCCTCCCTTTGATGGGTCGCCGGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtc < 6:152831/149‑1 (MQ=255) cccccTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtcatca < 5:168447/149‑1 (MQ=255) ccctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGCCGCCCCGCG‑ATAACGTTCTGCACACCTGGTACGTTTACCACTGAGAAGCTTGGCataatc > 7:143160/1‑149 (MQ=255) ccctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGCATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTcatcat < 1:84496/149‑1 (MQ=255) cctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCGGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATcc > 7:163675/1‑149 (MQ=255) ctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCAt < 2:234147/132‑1 (MQ=255) aTGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCcg < 3:79745/85‑1 (MQ=255) ggTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCTGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCt < 8:163675/149‑1 (MQ=255) gTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTg < 6:72372/149‑1 (MQ=255) tCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt < 8:137647/136‑1 (MQ=255) tCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCt > 7:137647/1‑136 (MQ=255) ccTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCt > 4:164544/1‑147 (MQ=255) cTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACCCCTGCTACGTTTACCAGTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg > 3:275780/1‑149 (MQ=255) cccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtc < 7:121816/114‑1 (MQ=255) cccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtc > 8:121816/1‑114 (MQ=255) ccTTTCGCGGGCTGCCGGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACCCCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGc > 6:247900/1‑148 (MQ=255) ccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc > 6:30245/1‑108 (MQ=255) ccTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGc < 5:30245/108‑1 (MQ=255) cTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGCCAGTAAACATCCCATGTTACAGACGCCACGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCAATCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCg > 6:53286/1‑148 (MQ=255) cTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa > 3:80901/1‑149 (MQ=255) tttCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg < 3:228163/137‑1 (MQ=255) tttCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg > 4:228163/1‑137 (MQ=255) | GTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCG‑ATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGA > NC_002947/2272928‑2273203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |