Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 197,590 T→C 14.0% D1032D (GAT→GAC PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947197,5900TC14.0% 235.0 / 26.1 100D1032D (GAT→GACPP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base C (4/10);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 108 total aligned reads displayed.

CCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACT  >  NC_002947/197442‑197737
                                                                                                                                                     |                                                                                                                                                     
cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAt                                                                                                                                                       <  2:193742/149‑1 (MQ=37)
cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAt                                                                                                                                                       <  5:93250/149‑1 (MQ=37)
 ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAtt                                                                                                                                                      >  1:152276/1‑149 (MQ=37)
 ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAtt                                                                                                                                                      >  1:157395/1‑149 (MQ=37)
     aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTcc                                                                                                                                                   <  4:82247/148‑1 (MQ=37)
          gAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGAccc                                                                                                                                             >  1:137927/1‑149 (MQ=37)
              ccGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAt                                                                                                                                                       >  8:142128/1‑135 (MQ=37)
              ccGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAt                                                                                                                                                       <  7:142128/135‑1 (MQ=37)
                   ggTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                     >  8:300789/1‑148 (MQ=37)
                     tCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GAc                                                                                                                                <  2:300175/149‑1 (MQ=37)
                     tCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GAc                                                                                                                                <  5:298649/149‑1 (MQ=37)
                         cGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTc                                                                                                                             >  3:63666/1‑148 (MQ=37)
                         cGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACACTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCAAGGGCAGCGACGATTTCCAGACCCCGGCGAAA‑‑GACGTc                                                                                                                             >  2:219168/1‑148 (MQ=255)
                           aaGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTa                                                                                                                           >  1:159055/1‑148 (MQ=37)
                                  cgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACg                                                                                                                   <  2:159055/149‑1 (MQ=37)
                                     gATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGtt                                                                                                                >  2:174044/1‑149 (MQ=37)
                                      atgaCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAAACATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGtt                                                                                                                <  1:219168/145‑1 (MQ=255)
                                         aCCTACCCCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa                                                                                                             <  2:157395/148‑1 (MQ=37)
                                         aCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa                                                                                                             <  6:19314/148‑1 (MQ=37)
                                         aCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa                                                                                                             <  1:174044/148‑1 (MQ=37)
                                         aCCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGa                                                                                                             <  2:152276/148‑1 (MQ=37)
                                           cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGAccg                                                                                                          >  2:12162/1‑147 (MQ=25)
                                           cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTg                                                                                                          >  5:55619/1‑149 (MQ=38)
                                             acacCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAtt                                                                                                                                                      <  1:211760/105‑1 (MQ=40)
                                             acacCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAtt                                                                                                                                                      >  2:211760/1‑105 (MQ=40)
                                              cacCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCa                                                                                                       <  7:300789/149‑1 (MQ=39)
                                                 cGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCg                                                                                                    >  1:69086/1‑149 (MQ=39)
                                                 cGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCg                                                                                                    >  5:187322/1‑149 (MQ=21)
                                                   tGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTc                                                                                                  <  3:78933/149‑1 (MQ=21)
                                                     aTCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCa                                                                                                 <  3:159506/148‑1 (MQ=18)
                                                       ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCa                                                                                              >  8:69459/1‑149 (MQ=37)
                                                       ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCa                                                                                              >  5:115947/1‑149 (MQ=17)
                                                        cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCa                                                                                              >  6:339914/1‑148 (MQ=17)
                                                         tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                                                            <  2:69086/149‑1 (MQ=37)
                                                         tGAGCAATCCTGCCCAGACGCCGGAAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                                                            >  5:231775/1‑149 (MQ=37)
                                                           aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCTATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                                                            <  4:142246/147‑1 (MQ=37)
                                                           aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGTCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATc                                                                                            >  8:68717/1‑147 (MQ=37)
                                                           aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCga                                                                                          <  3:237283/149‑1 (MQ=17)
                                                           aGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCAAGGCCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCCTCAGCGTCACCATc                                                                                            >  1:128849/1‑147 (MQ=255)
                                                             cAATCCTGCCCAGACGCCGGTAACCGTGACGCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑g                                                                                                                                  >  1:29042/1‑107 (MQ=25)
                                                             cAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑g                                                                                                                                  <  2:29042/107‑1 (MQ=35)
                                                             cAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgag                                                                                         >  3:301354/1‑148 (MQ=37)
                                                                tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAAc                                                                                      >  4:68781/1‑148 (MQ=37)
                                                                tCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAAc                                                                                      <  3:68781/148‑1 (MQ=37)
                                                                 ccTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAACCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGc                                                                                                     >  3:209135/1‑132 (MQ=37)
                                                                   tGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCca                                                                                  >  2:307518/1‑149 (MQ=37)
                                                                      ccAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCAATACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAg                                                                               >  5:99165/1‑149 (MQ=17)
                                                                        aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGc                                                                             <  7:270043/149‑1 (MQ=17)
                                                                        aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGc                                                                             <  6:115947/149‑1 (MQ=17)
                                                                          aCGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCgg                                                                           >  8:103008/1‑149 (MQ=255)
                                                                          aCGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCgg                                                                           >  2:320380/1‑149 (MQ=17)
                                                                           cGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGt                                                                          <  7:103008/149‑1 (MQ=255)
                                                                            gCCGTTGACCGTGACCCTGTCCAACGTCCAAACGATTACCGTTTAAGCCGGCTAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTAGAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTa                                                                         <  5:13716/149‑1 (MQ=11)
                                                                                gTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAttt                                                                     <  6:187322/149‑1 (MQ=17)
                                                                                 tgaCCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTc                                                                    <  4:207648/147‑1 (MQ=255)
                                                                                   aCCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                                                                  <  2:277864/149‑1 (MQ=34)
                                                                                   aCCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                                                                  <  6:67015/149‑1 (MQ=34)
                                                                                   aCCGTGACCCTGACCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                                                                  <  7:199823/149‑1 (MQ=255)
                                                                                     cGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg                                                                 >  1:227537/1‑148 (MQ=32)
                                                                                     cGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg                                                                 >  4:313807/1‑148 (MQ=32)
                                                                                     cGTGACCCTCTCCAACGGCCAAACTCTTAGCGTTGAAGCCGCCAAAACACAGGGCAGCGTCGACTTCCAGACCCCGGCGAATTCCTCGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                                                                  <  1:272302/149‑1 (MQ=11)
                                                                                      gTGACCCTGTCCAACGGCCAAACCATTACCGTTGAGAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg                                                                 >  1:50557/1‑148 (MQ=17)
                                                                                           ccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGCCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa                                                          >  2:211919/1‑149 (MQ=17)
                                                                                           ccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTg                                                           >  4:48493/1‑148 (MQ=32)
                                                                                           ccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa                                                          >  3:63171/1‑149 (MQ=32)
                                                                                           ccTGTCAAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa                                                          >  1:87079/1‑149 (MQ=17)
                                                                                            cTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgaga                                                                                        <  4:195566/118‑1 (MQ=32)
                                                                                            cTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgaga                                                                                        >  3:195566/1‑118 (MQ=32)
                                                                                                 cAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCg                                                     >  3:138846/1‑148 (MQ=12)
                                                                                                  aaCGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCTAGCAACTGACCCCGaa                                                   <  6:55619/149‑1 (MQ=17)
                                                                                                  aaCGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGaa                                                   >  8:227749/1‑149 (MQ=32)
                                                                                                    cGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAcc                                                 <  7:69459/149‑1 (MQ=33)
                                                                                                         aaaCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACg                                            >  7:198715/1‑149 (MQ=35)
                                                                                                           aCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGcc                                          <  2:50557/149‑1 (MQ=37)
                                                                                                             cATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCgg                                        <  7:68717/149‑1 (MQ=37)
                                                                                                               ttACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt                                      >  4:247854/1‑149 (MQ=37)
                                                                                                                   cGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGa                                  <  4:138846/149‑1 (MQ=12)
                                                                                                                     ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt                                      <  8:72905/143‑1 (MQ=37)
                                                                                                                     ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt                                      >  7:72905/1‑143 (MQ=37)
                                                                                                                       gAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAc                              >  2:153834/1‑149 (MQ=37)
                                                                                                                        aaGCCGGCAAAACCCAGTGCAGCGTCGACTTCCAGACCCCTGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACg                             <  1:210653/149‑1 (MQ=11)
                                                                                                                            ccGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGAt                           >  7:22579/1‑148 (MQ=37)
                                                                                                                             cGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATc                          >  7:128362/1‑148 (MQ=12)
                                                                                                                              ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAc                        <  3:313807/149‑1 (MQ=38)
                                                                                                                              ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAc                        <  4:63666/149‑1 (MQ=38)
                                                                                                                              ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATCTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGAc                                 <  6:295370/140‑1 (MQ=255)
                                                                                                                              ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATCTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCCGATcgg                        >  5:295370/1‑146 (MQ=11)
                                                                                                                                 aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTaa                                                                        <  2:148507/98‑1 (MQ=33)
                                                                                                                                 aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTaa                                                                        >  1:148507/1‑98 (MQ=33)
                                                                                                                                 aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGa                     <  2:137927/149‑1 (MQ=39)
                                                                                                                                   gACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACt                   <  2:227537/149‑1 (MQ=39)
                                                                                                                                     cccAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACACCGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCg                 >  5:102976/1‑149 (MQ=255)
                                                                                                                                      ccAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACGACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCgg                >  6:332118/1‑149 (MQ=255)
                                                                                                                                        aGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTc              <  3:48493/149‑1 (MQ=39)
                                                                                                                                            cAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGaca          >  6:87166/1‑149 (MQ=39)
                                                                                                                                             aGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGacac         <  7:227749/149‑1 (MQ=39)
                                                                                                                                               cGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGAcacca       >  5:332147/1‑149 (MQ=39)
                                                                                                                                                   gATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCactac   >  2:128602/1‑149 (MQ=39)
                                                                                                                                                   gATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCactac   >  1:81891/1‑149 (MQ=39)
                                                                                                                                                    aTTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCactact  >  8:46522/1‑149 (MQ=40)
                                                                                                                                                     |                                                                                                                                                     
CCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAT‑‑GACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACT  >  NC_002947/197442‑197737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: