Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,308,317	G→C	5.4%	A251A (GCC→GCG)	PP_2031 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,308,317	0	G	C	5.4%	242.6 / 3.4	74	A251A (GCC→GCG)	PP_2031	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/36);  new base C (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

TTCACTTTGCGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCTGAGCGAGCGTTCATCGCAGATCACGAT  >  NC_002947/2308170‑2308460                                                                                                                                                    |                                                                                                                                                 ttCACTTTGCGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTggg                                                                                                                                                 >  1:116076/1‑149 (MQ=255)  tCACTTTGCGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTggg                                                                                                                                                 <  5:133682/148‑1 (MQ=255)   cACTTTGCGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGc                                                                                                                                                >  2:238631/1‑148 (MQ=255)     cTTTGCGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGaa                                                                                                                                             <  2:123173/149‑1 (MQ=255)       ttGCGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAgc                                                                                                                                           >  1:276896/1‑149 (MQ=255)          cGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCgca                                                                                                                                        <  2:230461/149‑1 (MQ=255)           gTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCgcag                                                                                                                                       >  4:86933/1‑149 (MQ=255)               tCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAgg                                                                                                                                   >  4:26899/1‑149 (MQ=255)               tCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAgg                                                                                                                                   >  3:141965/1‑149 (MQ=255)                       aTCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCcggcgg                                                                                                                            >  6:4567/1‑148 (MQ=255)                          aGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCggcagg                                                                                                                        <  8:270800/149‑1 (MQ=255)                          aGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCggcag                                                                                                                         <  8:130399/148‑1 (MQ=255)                           gCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCggcagg                                                                                                                        >  6:241265/1‑148 (MQ=255)                              ccaccCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGTGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAAc                                                                                                                    <  6:129873/149‑1 (MQ=255)                               caccCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGaa                                                                                                                                             <  8:22201/123‑1 (MQ=255)                                 cccTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAAcgcg                                                                                                                 <  4:31430/149‑1 (MQ=255)                                  ccTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGt                                                                                                                <  3:177636/149‑1 (MQ=255)                                   cTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTCTCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCcg                                                                                                                                <  3:233377/132‑1 (MQ=255)                                   cTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCcg                                                                                                                                >  4:233377/1‑132 (MQ=255)                                    tCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGcagcag                                                                                                                                    >  2:82412/1‑127 (MQ=255)                                    tCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGcagcag                                                                                                                                    <  1:82412/127‑1 (MQ=255)                                     cAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGt                                                                                                             >  7:306229/1‑149 (MQ=255)                                      aGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTgg                                                                                                              <  1:129437/147‑1 (MQ=255)                                      aGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGGTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTc                                                                                                            >  6:73980/1‑149 (MQ=255)                                           ttGCGCCATGAGCTCCCAGCTCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGc                                                                                                        <  1:72301/148‑1 (MQ=255)                                           ttGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGc                                                                                                        <  2:276896/148‑1 (MQ=255)                                            tGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCg                                                                                                       <  4:310076/148‑1 (MQ=255)                                              cgcCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCggg                                                                                                     <  3:99737/148‑1 (MQ=255)                                               gcCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTTCGCCCAGCgcagt                                                                                                                                      <  2:15198/114‑2 (MQ=255)                                               gcCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTTCGCCCAGCgcagt                                                                                                                                      >  1:15198/1‑113 (MQ=255)                                               gcCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTc                                                                                                   >  1:253214/1‑149 (MQ=255)                                                ccATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑g                                                                                                 <  8:306229/149‑1 (MQ=255)                                                ccATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑g                                                                                                 >  1:331948/1‑149 (MQ=255)                                                ccATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑g                                                                                                 >  4:312465/1‑149 (MQ=255)                                                 cATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑g                                                                                                 >  8:142173/1‑148 (MQ=255)                                                  aTCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGa                                                                                               <  5:4567/149‑1 (MQ=255)                                                     aGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATAc                                                                                            <  2:116076/149‑1 (MQ=255)                                                       cTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCggcagg                                                                                                                        >  3:49955/1‑120 (MQ=255)                                                       cTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCggcagg                                                                                                                        <  4:49955/120‑1 (MQ=255)                                                          ccAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCgg                                                                                         <  5:241265/147‑1 (MQ=255)                                                            aGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGgcag                                                                                      >  2:103901/1‑148 (MQ=255)                                                            aGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGgcag                                                                                      >  2:107908/1‑148 (MQ=255)                                                              cgcTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTTCGCCCAGCgcagtgc                                                                                                                                    <  7:14301/101‑4 (MQ=255)                                                              cgcTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTTCGCCCAGCgcagtgc                                                                                                                                    >  8:14301/1‑98 (MQ=255)                                                                cTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAAca                                                                                 <  7:201839/149‑1 (MQ=255)                                                                   gATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGcagcagcag                                                                                <  2:331948/147‑1 (MQ=255)                                                                   gATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAAcag                                                                                >  2:191365/1‑147 (MQ=255)                                                                   gATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAAcag                                                                                >  7:314943/1‑147 (MQ=255)                                                                   gATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAAcag                                                                                >  3:86029/1‑147 (MQ=255)                                                                   gATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAAcag                                                                                >  3:65349/1‑147 (MQ=255)                                                                       cGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTc                                                                          >  2:323528/1‑149 (MQ=255)                                                                         ggCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGc                                                                        <  3:86933/149‑1 (MQ=255)                                                                          gCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGc                                                                        >  2:91425/1‑148 (MQ=255)                                                                               ggCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGc                                                                   <  4:280189/148‑1 (MQ=255)                                                                                 ccTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGt                                                                <  3:26899/149‑1 (MQ=255)                                                                                          ggTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATg                                                       >  3:224251/1‑149 (MQ=255)                                                                                                    gcgcAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCNGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCCCAATGCCGTGAGAGCATGGCCAGGTg                                               <  1:107908/147‑1 (MQ=255)                                                                                                    gcgcAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGc                                              <  5:73980/148‑1 (MQ=255)                                                                                                    gcgcAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCCCAATGCCGTGAGAGCATGGCCAGGTg                                               <  1:103901/147‑1 (MQ=255)                                                                                                      gcAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGTCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGGAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGt                                           <  1:238631/149‑1 (MQ=255)                                                                                                       cAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGtt                                          >  2:251218/1‑149 (MQ=255)                                                                                                        aGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTc                                         >  6:200173/1‑149 (MQ=255)                                                                                                          gTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCa                                       <  1:191365/149‑1 (MQ=255)                                                                                                            tGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCgg                                                                                         <  6:180387/97‑1 (MQ=255)                                                                                                            tGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCgg                                                                                         >  5:180387/1‑97 (MQ=255)                                                                                                            tGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCAcc                                     >  5:236240/1‑149 (MQ=255)                                                                                                                gTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCCGTTCCACCTggg                                 <  1:323528/149‑1 (MQ=255)                                                                                                                    aGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCt                             >  6:224174/1‑149 (MQ=255)                                                                                                                                  cAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTgg                                  >  8:82784/1‑130 (MQ=255)                                                                                                                                  cAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTgg                                  <  7:82784/130‑1 (MQ=255)                                                                                                                                  cAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGGTGAGCGAGCGTGCAt               >  2:270792/1‑149 (MQ=255)                                                                                                                                    gCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCTGAGCGAGCGTTCATCg             <  4:65349/149‑1 (MQ=255)                                                                                                                                      tGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCTGAGCGAGCGTTCATCGCa           <  2:253214/149‑1 (MQ=255)                                                                                                                                       gggCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCTGAGCGAGCGTTCATCg             >  5:36263/1‑146 (MQ=255)                                                                                                                                               cgcTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCTGAGCGAGCGTTCATCGCAGATCACGAt  <  7:142173/149‑1 (MQ=255)                                                                                                                                                gcTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTCGGGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCTGAGCGAGCGTTCATCGCAGATCACGAt  >  1:89151/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TTCACTTTGCGTGCTCAGAGGCATCAGCACCACCCTCAGCTTTTGCGCCATCAGCTCCCAGCGCTCGATGCGGGCTTCGGCCTGCTGGCGGTAGGCCTGGCGCAGGTTGGCGTCGAGGGTATCGAGCTCCAGCTGGGCACTGCGCTGGGCGAAGCGCAGCAGGCCGGCGGCAGGCAACGCGTGGTCGAGCGGGTC‑GGATACCGGCAGCAACAGCAGGTCGCAATGCCGTGAGAGCATGGCCAGGTGCTGTTCCACCTGGGCGCTGAGCGAGCGTTCATCGCAGATCACGAT  >  NC_002947/2308170‑2308460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑