Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,311,048 G→A 7.7% V267I (GTA→ATA) ‡ PP_2033 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,311,0480GA 7.7% 152.8 / 2.4 52V267I (GTA→ATA) ‡PP_2033hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base A (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GAGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGC  >  NC_002947/2310901‑2311189
                                                                                                                                                   |                                                                                                                                             
gAGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACCTCGTGCTTCCGTGCGTGCACTCGAATATTGGCGGTGTTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGAGCGTGCCTGt                                                                                                                                              <  6:327599/149‑1 (MQ=255)
            cggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCgtgt                                                                                                                                  <  6:193788/149‑1 (MQ=255)
             ggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcactgg                                                                                                                                 <  3:275954/149‑6 (MQ=255)
                aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTc                                                                                                                                 >  3:37578/1‑146 (MQ=255)
                aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGa                                                                                                                               <  4:37578/148‑1 (MQ=255)
                aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAg                                                                                                                              <  4:160967/149‑1 (MQ=255)
                    cTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGt                                                                                                                          >  7:279352/1‑149 (MQ=255)
                     ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTg                                                                                                                         >  5:244135/1‑149 (MQ=255)
                        ccGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGcc                                                                                                                       <  6:33000/148‑1 (MQ=255)
                                 cgcAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGc                                                                                                             >  5:63595/1‑149 (MQ=255)
                                  gcAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGcc                                                                                                            <  2:326456/149‑1 (MQ=255)
                                    aGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCaa                                                                                                          <  7:133408/149‑1 (MQ=255)
                                       ggCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                       <  6:302531/149‑1 (MQ=255)
                                        gCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                       <  3:322979/148‑1 (MQ=255)
                                        gCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                       <  6:63595/148‑1 (MQ=255)
                                                    tgctgcCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTg                                                                                          >  7:47613/1‑149 (MQ=255)
                                                    tgctgcCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTg                                                                                          >  6:337612/1‑149 (MQ=255)
                                                            ggCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGc                                                                                  <  5:337612/149‑1 (MQ=255)
                                                                  cACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGc                                                                            <  7:66656/149‑1 (MQ=255)
                                                                   aCTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                              >  5:149171/1‑146 (MQ=255)
                                                                   aCTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                              <  6:149171/146‑1 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCATTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGc                                                                                                             <  7:165076/113‑1 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                         >  4:107187/1‑149 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                         >  6:200560/1‑149 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                         >  8:66560/1‑149 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAATCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGc                                                                                                             >  8:165076/1‑113 (MQ=255)
                                                                            ttGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                                  <  7:66560/149‑1 (MQ=255)
                                                                            ttGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                                  <  1:49065/149‑1 (MQ=255)
                                                                                     gtTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAgg                                                         <  8:74526/149‑1 (MQ=255)
                                                                                                 cccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatg                                             <  8:47613/149‑1 (MQ=255)
                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCt                                                                                           >  6:154260/1‑102 (MQ=255)
                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                            >  5:7695/1‑149 (MQ=255)
                                                                                                         gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTg                                        <  6:7695/146‑1 (MQ=255)
                                                                                                         gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGgga                                     <  6:244135/149‑1 (MQ=255)
                                                                                                          aGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggag                                    <  3:107187/149‑1 (MQ=255)
                                                                                                              ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                >  6:45854/1‑149 (MQ=255)
                                                                                                              ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                >  6:50788/1‑149 (MQ=255)
                                                                                                                  ttGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAt                            >  7:250179/1‑149 (MQ=255)
                                                                                                                      tgtgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGat                                                                        <  7:246862/101‑1 (MQ=255)
                                                                                                                      tgtgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGat                                                                        >  8:246862/1‑101 (MQ=255)
                                                                                                                          cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                    >  3:2917/1‑149 (MQ=255)
                                                                                                                          cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                    >  4:31797/1‑149 (MQ=255)
                                                                                                                          cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                    <  8:279352/149‑1 (MQ=255)
                                                                                                                               cGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcgg               >  4:110382/1‑149 (MQ=255)
                                                                                                                                gCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcggc              >  4:204628/1‑149 (MQ=255)
                                                                                                                                      ccAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACATGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc         >  4:227741/1‑148 (MQ=255)
                                                                                                                                       acacgcgtgcCTACAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc         >  5:274091/5‑147 (MQ=255)
                                                                                                                                        aGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAg      >  4:110778/1‑149 (MQ=255)
                                                                                                                                         acgcgtgcCTACAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAgg     >  1:257650/3‑149 (MQ=255)
                                                                                                                                         acgcgtgcCTACAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAgg     <  2:257650/147‑1 (MQ=255)
                                                                                                                                             gtgcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgcgc  <  2:243350/148‑1 (MQ=255)
                                                                                                                                             gtgcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgcgc  >  1:243350/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GAGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGC  >  NC_002947/2310901‑2311189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑