Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,311,049 T→C 7.8% V267A (GTA→GCA) ‡ PP_2033 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,311,0490TC 7.8% 142.1 / 3.2 51V267A (GTA→GCA) ‡PP_2033hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GAGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310901‑2311197
                                                                                                                                                    |                                                                                                                                                    
gAGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACCTCGTGCTTCCGTGCGTGCACTCGAATATTGGCGGTGTTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGAGCGTGCCTGt                                                                                                                                                      <  6:327599/149‑1 (MQ=255)
            cggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCgtgt                                                                                                                                          <  6:193788/149‑1 (MQ=255)
             ggcATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTACAGGCAcgcactgg                                                                                                                                         <  3:275954/149‑6 (MQ=255)
                aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTc                                                                                                                                         >  3:37578/1‑146 (MQ=255)
                aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGa                                                                                                                                       <  4:37578/148‑1 (MQ=255)
                aTAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAg                                                                                                                                      <  4:160967/149‑1 (MQ=255)
                    cTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGt                                                                                                                                  >  7:279352/1‑149 (MQ=255)
                     ttCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTg                                                                                                                                 >  5:244135/1‑149 (MQ=255)
                        ccGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGcc                                                                                                                               <  6:33000/148‑1 (MQ=255)
                                 cgcAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGc                                                                                                                     >  5:63595/1‑149 (MQ=255)
                                  gcAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGcc                                                                                                                    <  2:326456/149‑1 (MQ=255)
                                    aGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCaa                                                                                                                  <  7:133408/149‑1 (MQ=255)
                                       ggCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                               <  6:302531/149‑1 (MQ=255)
                                        gCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                               <  3:322979/148‑1 (MQ=255)
                                        gCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGt                                                                                                               <  6:63595/148‑1 (MQ=255)
                                                    tgctgcCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTg                                                                                                  >  6:337612/1‑149 (MQ=255)
                                                    tgctgcCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTg                                                                                                  >  7:47613/1‑149 (MQ=255)
                                                            ggCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGc                                                                                          <  5:337612/149‑1 (MQ=255)
                                                                  cACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGc                                                                                    <  7:66656/149‑1 (MQ=255)
                                                                   aCTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                                      <  6:149171/146‑1 (MQ=255)
                                                                   aCTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGttt                                                                                      >  5:149171/1‑146 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCATTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGc                                                                                                                     <  7:165076/113‑1 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  6:200560/1‑149 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  4:107187/1‑149 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGa                                                                                 >  8:66560/1‑149 (MQ=255)
                                                                     tCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAATCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGc                                                                                                                     >  8:165076/1‑113 (MQ=255)
                                                                            ttGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                                          <  7:66560/149‑1 (MQ=255)
                                                                            ttGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGc                                                                          <  1:49065/149‑1 (MQ=255)
                                                                                     gtTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAgg                                                                 <  8:74526/149‑1 (MQ=255)
                                                                                                 cccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatg                                                     <  8:47613/149‑1 (MQ=255)
                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCt                                                                                                   >  6:154260/1‑102 (MQ=255)
                                                                                                  ccTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGtgatga                                                    >  5:7695/1‑149 (MQ=255)
                                                                                                         gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTg                                                <  6:7695/146‑1 (MQ=255)
                                                                                                         gAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGgga                                             <  6:244135/149‑1 (MQ=255)
                                                                                                          aGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGggag                                            <  3:107187/149‑1 (MQ=255)
                                                                                                              ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                        >  6:45854/1‑149 (MQ=255)
                                                                                                              ggTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGc                                        >  6:50788/1‑149 (MQ=255)
                                                                                                                  ttGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGAt                                    >  7:250179/1‑149 (MQ=255)
                                                                                                                      tgtgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGat                                                                                >  8:246862/1‑101 (MQ=255)
                                                                                                                      tgtgCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGat                                                                                <  7:246862/101‑1 (MQ=255)
                                                                                                                          cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                            <  8:279352/149‑1 (MQ=255)
                                                                                                                          cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                            >  4:31797/1‑149 (MQ=255)
                                                                                                                          cAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTg                            >  3:2917/1‑149 (MQ=255)
                                                                                                                               cGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcgg                       >  4:110382/1‑149 (MQ=255)
                                                                                                                                gCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGggcggc                      >  4:204628/1‑149 (MQ=255)
                                                                                                                                      ccAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACATGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc                 >  4:227741/1‑148 (MQ=255)
                                                                                                                                       acacgcgtgcCTACAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGAc                 >  5:274091/5‑147 (MQ=255)
                                                                                                                                        aGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAg              >  4:110778/1‑149 (MQ=255)
                                                                                                                                         acgcgtgcCTACAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAgg             <  2:257650/147‑1 (MQ=255)
                                                                                                                                         acgcgtgcCTACAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAgg             >  1:257650/3‑149 (MQ=255)
                                                                                                                                             gtgcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgcgc          <  2:243350/148‑1 (MQ=255)
                                                                                                                                             gtgcCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGgcgc          >  1:243350/1‑148 (MQ=255)
                                                                                                                                                    tAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCa   <  3:204628/148‑1 (MQ=255)
                                                                                                                                                    tAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAg  <  5:200560/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGGACGAGCGGCGGCATAACTTCCCGCTGGTGCGCAGTGGCCACGACATCGTGCTGCCGGGCGTGCACTCGAATATTGGGGGTGGTTACCCGGAGACCCTTCTGGAGCAGGTGTTGCTGTGCAAGCCGCAGTCCCAGTGCGTGCCTGTAGGCACGCGTGTCGAGCAGTGCCGCGTACATGCCAAGGTGAGTGCCTTGCTGGCTTCGGCGTTTGCCGATATGGGCGAACCCAGGCCGCGCGTGATGACCTGGGAGGAGCCGATTGCCGGTGGGCGGCGGGACGAGGCGCAAAAGCAG  >  NC_002947/2310901‑2311197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑