Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,337,203 C→G 8.1% V287L (GTT→CTT)  ybhD ← DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,337,2030CG 8.1% 153.1 / 2.6 49V287L (GTT→CTT) ybhDDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (23/22);  new base G (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

AGTACTTCTGCGATCAGTACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGACACGC  >  NC_002947/2337055‑2337349
                                                                                                                                                    |                                                                                                                                                  
aGTACTTCTGCGATCAGTACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAACGCCGCGCGCGGCTACCTTCAGCGATTTGGGCATTTCTGCTGCAGGATCGAAAc                                                                                                                                                    >  5:106831/1‑149 (MQ=255)
    cTTCTGCGATCAGTACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAAt                                                                                                                                                <  5:145357/149‑1 (MQ=255)
    cTTCTGCGATCAGTACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCAGCAGCAGGATCGAAACGAAt                                                                                                                                                <  8:115044/149‑1 (MQ=255)
         gCGATCAGTACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTtctc                                                                                                                                           >  3:79110/1‑149 (MQ=255)
                gTACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGcc                                                                                                                                    >  6:191222/1‑149 (MQ=255)
                 tACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCg                                                                                                                                   >  5:15790/1‑149 (MQ=255)
                       atgatgCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACa                                                                                                                             <  5:180682/149‑1 (MQ=255)
                        tgatgCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGCACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAg                                                                                                                            <  2:33925/149‑1 (MQ=255)
                                      aaCCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATgcgccgcgcc                                                                                                              <  8:13132/149‑1 (MQ=255)
                                                  cATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGcc                                                                                                  <  6:244250/149‑1 (MQ=255)
                                                  cATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGcc                                                                                                  <  4:185843/149‑1 (MQ=255)
                                                   atatCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGccc                                                                                                 >  4:76476/1‑149 (MQ=255)
                                                        gAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCGGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCg                                                                                            >  5:165908/1‑149 (MQ=255)
                                                        gAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCg                                                                                            >  6:125752/1‑149 (MQ=255)
                                                           cATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGc                                                                                         <  3:76476/149‑1 (MQ=255)
                                                             ttttGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCATCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGTGCCGGTACACCAGCCCTAGCGTGCgg                                                                                       <  1:181279/149‑1 (MQ=255)
                                                                 gACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAAGGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGa                                                                                   >  1:221080/1‑149 (MQ=255)
                                                                 gACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAAGGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGa                                                                                   >  1:225826/1‑149 (MQ=255)
                                                                                 gTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGCTCaa                                                                   >  2:271905/1‑149 (MQ=255)
                                                                                          aCTATGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGAc                                                                                  <  5:232957/125‑1 (MQ=255)
                                                                                           cTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCt                                                          >  4:123266/1‑148 (MQ=255)
                                                                                              cggCCGCGCGCGGCGGCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACTGCGATGCGCCGCGCCGGTACACGAGGCCTAGCGTGCGGGTGAGTTGCGGCTCGATCTACGGCTCGCTCAc                                                       <  6:106831/147‑1 (MQ=255)
                                                                                                gCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTg                                                                               <  3:171679/122‑1 (MQ=255)
                                                                                                gCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTg                                                                               >  4:171679/1‑122 (MQ=255)
                                                                                                 ccGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGt                                                                                     <  8:229041/115‑1 (MQ=255)
                                                                                                 ccGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGt                                                                                     >  7:229041/1‑115 (MQ=255)
                                                                                                 ccGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCaa                                                                   >  8:295306/1‑133 (MQ=255)
                                                                                                 ccGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCaa                                                                   <  7:295306/133‑1 (MQ=255)
                                                                                                   gcgcgcgGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGt                                                 >  1:212437/1‑149 (MQ=255)
                                                                                                       gcgGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAAGGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTgggg                                             <  2:225826/149‑1 (MQ=255)
                                                                                                       gcgGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAAGGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTgggg                                             <  2:221080/149‑1 (MQ=255)
                                                                                                               cttcAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCgg                                      <  5:191222/148‑1 (MQ=255)
                                                                                                                   aGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTg                                 <  4:79110/149‑1 (MQ=255)
                                                                                                                   aGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTg                                 >  5:321960/1‑149 (MQ=255)
                                                                                                                    gCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTgg                                >  5:185399/1‑149 (MQ=255)
                                                                                                                           ggCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCt                                                                                                >  3:322975/1‑78 (MQ=255)
                                                                                                                           ggCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCt                                                                                                <  4:322975/78‑1 (MQ=255)
                                                                                                                            gCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATg                           <  8:286621/146‑1 (MQ=255)
                                                                                                                            gCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATg                           >  7:286621/1‑146 (MQ=255)
                                                                                                                            gCCATTTCTGCAGCAGGATCGAAACGAATTTCTCAGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGc                         >  4:127872/1‑148 (MQ=255)
                                                                                                                               aTTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAgg                     >  8:158239/1‑149 (MQ=255)
                                                                                                                                         cagGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATgg           >  7:42269/1‑149 (MQ=255)
                                                                                                                                              tCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGac      <  2:75235/149‑1 (MQ=255)
                                                                                                                                              tCGAAACGAATTTCTCAGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGac      >  3:232489/1‑149 (MQ=255)
                                                                                                                                                gAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGacac    <  6:185399/149‑1 (MQ=255)
                                                                                                                                                 aaaCGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCTGGTACACCAGCCCTAGCGTGTGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGACACg   >  6:190732/1‑149 (MQ=255)
                                                                                                                                                 aaaCGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGACACg   >  7:109921/1‑149 (MQ=255)
                                                                                                                                                 aaaCGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGACACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCa                       <  1:166489/129‑1 (MQ=255)
                                                                                                                                                 aaaCGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGACACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCa                       >  2:166489/1‑129 (MQ=255)
                                                                                                                                                  aaCGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGACACGc  <  2:275374/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGTACTTCTGCGATCAGTACCGGATGATGCTCGAGGACAACCTCGATGAGCATATCGAGCATTTTGACCGGTACATGCTGGGTAAGAACGACTAAGGCCGCGCGCGGCTTCCTTCAGCGATTTGGCCATTTCTGCAGCAGGATCGAAACGAATTTCTCCGCAGCCGGCGACAGCGATGCGCCGCGCCGGTACACCAGCCCTAGCGTGCGGGTGACTTGCGGCTCGATCAACGGCACGCTCACCAGCGTGGGGTGGTCGGCATTGGGCATGGCCAGGCTGGGCATGGCCGACACGC  >  NC_002947/2337055‑2337349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑