Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,369,767 T→C 10.2% L862P (CTG→CCG) ‡ gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,369,7670TC10.2% 127.3 / 5.6 50L862P (CTG→CCG) ‡gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (19/25);  new base C (2/3);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

AACGTTGCCCGTGGTGGCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATGACGAC  >  NC_002947/2369619‑2369909
                                                                                                                                                     |                                                                                                                                                  
aaCGTTGCCCGTGGTGGCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTCCCGAAGTGCTGTGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCt                                                                                                                                                    <  8:280820/149‑1 (MQ=255)
        ccGTGGTGGCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCa                                                                                                                                            <  4:224088/149‑1 (MQ=255)
           tggtggCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCCCAGCGGCAggc                                                                                                                                         <  1:332729/149‑3 (MQ=255)
           tggtggCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCCCAGCGGCAggc                                                                                                                                         <  7:141722/149‑3 (MQ=255)
             gtggCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCCCAGCGGCAGGCga                                                                                                                                       <  6:100501/149‑2 (MQ=255)
              tggCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTg                                                                                                                                      <  5:64345/149‑1 (MQ=255)
                gCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGAc                                                                                                                                    <  4:285238/149‑1 (MQ=255)
                  tcttcgcggTCGGACCAGCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGAc                                                                                                                                    >  4:112355/8‑148 (MQ=255)
                     cgcTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACgag                                                                                                                                 <  8:230806/147‑1 (MQ=255)
                      gcTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATc                                                                                                                              <  8:78455/149‑1 (MQ=255)
                          gTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATcgccg                                                                                                                          >  3:277878/1‑149 (MQ=255)
                             cGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCgccgcc                                                                                                                        >  5:107401/1‑148 (MQ=255)
                               aCC‑GCGAAGAGGACTTGCGTAGCGTAGTGCTGTGGCTGGTGAAAGCCCAGCATGTGAAGAACTCGGTCAGGGTGACGGTCGTCTCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTTAGGTGATCGACGCCGaa                                                                                                                     <  6:188841/149‑1 (MQ=255)
                               aCC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGccgccg                                                                                                                       >  3:121610/1‑147 (MQ=255)
                               aCC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGccgccg                                                                                                                       >  3:211097/1‑147 (MQ=255)
                                   gcgAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAggc                                                                                                                  <  1:118794/149‑1 (MQ=255)
                                              ttCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTa                                                                                                   >  8:281713/1‑149 (MQ=255)
                                              ttCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGAGAAGAACTCGGTCATCGTGCCGGTCGGCGCCATGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTa                                                                                                   >  6:334650/1‑149 (MQ=255)
                                                 cGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCg                                                                                                >  6:112544/1‑149 (MQ=255)
                                                     ccGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATc                                                                                            <  5:112544/149‑1 (MQ=255)
                                                        aaGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAg                                                                                                                    >  8:309150/1‑126 (MQ=255)
                                                        aaGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAg                                                                                                                    <  7:309150/126‑1 (MQ=255)
                                                                   cTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCt                                                                              <  4:211097/149‑1 (MQ=255)
                                                                   cTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCt                                                                              <  4:121610/149‑1 (MQ=255)
                                                                      gTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGc                                                                            <  8:26015/148‑1 (MQ=255)
                                                                       tGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTc                                                                          <  7:162876/149‑1 (MQ=255)
                                                                                agcagGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCAcc                                                                 >  6:214235/1‑149 (MQ=255)
                                                                                     gTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTc                                                                                    <  4:275455/125‑1 (MQ=255)
                                                                                     gTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTc                                                                                    >  3:275455/1‑125 (MQ=255)
                                                                                     gTGAAGAACTCGGTCATCGTGCCGGCCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACaa                                                            >  6:34981/1‑149 (MQ=255)
                                                                                      tGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAAc                                                           <  3:112355/149‑1 (MQ=255)
                                                                                       gaagaaCTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAAcc                                                          >  3:91303/1‑149 (MQ=255)
                                                                                          gaaCTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCTCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCa                                                       <  6:107401/149‑1 (MQ=255)
                                                                                             cTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTACCGCGTCGCCTGCCGCTGGGCGGCCGCCCTGACGAGATAGCCCCCCAAGGC‑‑‑‑GTGGCGTGCTAACCCCAATTCATTTACGGCCAGCGCGACAAAACCCACAAccaaaag                                                     >  2:172877/1‑143 (MQ=255)
                                                                                                ggTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACg                                                 >  3:161837/1‑149 (MQ=255)
                                                                                                ggTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTACCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACg                                                 <  1:172877/149‑1 (MQ=255)
                                                                                                  tCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGt                                               <  7:281713/149‑1 (MQ=255)
                                                                                                      cGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCg                                           >  5:127251/1‑149 (MQ=255)
                                                                                                        tGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCgtg                                         >  7:114418/1‑149 (MQ=255)
                                                                                                            ggTCGGCGCCAAGGGGGGTTTTCTGCCGCGGCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGCCCCCTGTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCgtg                                         <  1:275983/149‑1 (MQ=255)
                                                                                                                   gcCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGc                              >  6:139740/1‑149 (MQ=255)
                                                                                                                    ccAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGcc                             <  4:91303/149‑1 (MQ=255)
                                                                                                                     cAAGGGTGGTTTCCTGCCGCGTCGCCTGGCGCAGGGCGGCAGCCGAGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGGGTGCTTCCGGATCGTCAATTACGGCCTGCAGGACCAAACCCCCAAACTCAACGACGGTGGGCTGGGGACGCCgggc                             >  6:196031/1‑146 (MQ=255)
                                                                                                                       tgggTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCGGTGAGGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCAGCTAGAACGTCTAGGACGGTGGCGTGGTTCGGCCGGCCaat                          <  6:127251/148‑2 (MQ=255)
                                                                                                                                     ccGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatg             <  5:334650/148‑1 (MQ=255)
                                                                                                                                         cacggcTGCCGCCCAGCGGCAGGCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgat        >  4:283502/6‑149 (MQ=255)
                                                                                                                                          acggcTGCCGCCCAGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgat        >  1:63076/5‑148 (MQ=255)
                                                                                                                                            gCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgatgac     >  7:245013/1‑149 (MQ=255)
                                                                                                                                            gCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgatgac     <  4:277878/149‑1 (MQ=255)
                                                                                                                                             ccTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCatgacgatgacg    <  4:161837/149‑1 (MQ=255)
                                                                                                                                                 ccGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATgacgac  <  8:245013/147‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AACGTTGCCCGTGGTGGCCTGCGCTGGTCCGACC‑GCGAAGAGGACTTCCGTACCGAAGTGCTGGGGCTGGTGAAAGCCCAGCAGGTGAAGAACTCGGTCATCGTGCCGGTCGGCGCCAAGGGTGGTTTCCTGCCGCGTCGCCTGCCGCTGGGCGGCAGCCGTGACGAGATCGCCGCCGAAGGC‑‑‑‑GTGGCGTGCTACCGCATCTTCATTTCCGGCCTGCTCGACATCACCGACAACCTCAAGGACGGTGGCGTGGTGCCGCCGGCCAACGTGGTGCGTCATGACGATGACGAC  >  NC_002947/2369619‑2369909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑