Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 197,695 G→C 9.1% T1067T (ACG→ACC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947197,6950GC 9.1% 186.5 / 5.2 63T1067T (ACG→ACC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (25/32);  new base C (2/4);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTC  >  NC_002947/197547‑197838
                                                                                                                                                    |                                                                                                                                                
aaaCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACg                                                                                                                                                  >  7:198715/1‑149 (MQ=35)
  aCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGcc                                                                                                                                                <  2:50557/149‑1 (MQ=37)
    gaTTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCgg                                                                                                                                              <  2:173990/148‑1 (MQ=12)
    cATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCgg                                                                                                                                              <  7:68717/149‑1 (MQ=37)
      ttACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt                                                                                                                                            >  4:247854/1‑149 (MQ=37)
          cGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGa                                                                                                                                        <  4:138846/149‑1 (MQ=12)
            ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt                                                                                                                                            <  8:72905/143‑1 (MQ=37)
            ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCt                                                                                                                                            >  7:72905/1‑143 (MQ=37)
              gAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAc                                                                                                                                    >  2:153834/1‑149 (MQ=37)
               aaGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGAccgc                                                                                                                                    <  1:307518/148‑3 (MQ=37)
               aaGCCGGCAAAACCCAGTGCAGCGTCGACTTCCAGACCCCTGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCAC‑g                                                                                                                                  <  1:210653/149‑1 (MQ=11)
                  ccGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GAt                                                                                                                                >  7:22579/1‑148 (MQ=37)
                   cGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCAC‑GATc                                                                                                                               >  7:128362/1‑148 (MQ=12)
                    ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCAc                                                                                                                             <  4:63666/149‑1 (MQ=38)
                    ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCAc                                                                                                                             <  3:313807/149‑1 (MQ=38)
                    ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATCTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGAc                                                                                                                                       <  6:295370/140‑1 (MQ=255)
                    ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATCTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACC‑C‑GATcgg                                                                                                                             >  5:295370/1‑146 (MQ=11)
                      cAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCg                                                                                                                           <  2:87079/149‑1 (MQ=39)
                       aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGa                                                                                                                          <  2:137927/149‑1 (MQ=39)
                         gACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACt                                                                                                                        <  2:227537/149‑1 (MQ=39)
                           cccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACACCGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCg                                                                                                                      >  5:102976/1‑149 (MQ=255)
                            ccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACGACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCgg                                                                                                                     >  6:332118/1‑149 (MQ=255)
                              aGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTc                                                                                                                   <  3:48493/149‑1 (MQ=39)
                                  cAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGaca                                                                                                               >  6:87166/1‑149 (MQ=39)
                                   aGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGacac                                                                                                              <  7:227749/149‑1 (MQ=39)
                                     cGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGAcacca                                                                                                            >  5:332147/1‑149 (MQ=39)
                                         gATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCactac                                                                                                        >  1:81891/1‑149 (MQ=39)
                                         gATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCactac                                                                                                        >  2:128602/1‑149 (MQ=39)
                                          aTTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCactact                                                                                                       >  8:46522/1‑149 (MQ=40)
                                                aGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCactact                                                                                                       <  6:326429/143‑1 (MQ=40)
                                                aGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCactact                                                                                                       >  5:326429/1‑143 (MQ=40)
                                                     ccGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGAc                                                                                            <  4:301354/149‑1 (MQ=255)
                                                        gCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGc                                                                                         <  1:153834/149‑1 (MQ=255)
                                                                       aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCg                                                                                                                      <  5:12636/105‑1 (MQ=40)
                                                                       aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCg                                                                                                                      >  6:12636/1‑105 (MQ=40)
                                                                       aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGAc                                                                          >  2:308775/1‑149 (MQ=255)
                                                                             ggTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAgg                                                                    >  4:238341/1‑149 (MQ=255)
                                                                             ggTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAgg                                                                    <  8:198715/149‑1 (MQ=255)
                                                                              gTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggc                                                                   <  6:332147/149‑1 (MQ=255)
                                                                                  gACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcggc                                                                <  5:332118/148‑1 (MQ=255)
                                                                                   aCTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGt                                                              >  5:286044/1‑149 (MQ=255)
                                                                                   aCTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGt                                                              <  8:22579/149‑1 (MQ=255)
                                                                                    cTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTg                                                             <  5:87166/149‑1 (MQ=255)
                                                                                    cTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTg                                                             <  2:81891/149‑1 (MQ=255)
                                                                                    cTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTg                                                             <  7:46522/149‑1 (MQ=255)
                                                                                          gCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCAcc                                                       <  1:211919/149‑1 (MQ=255)
                                                                                               aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGgcggcg                                                               >  1:151379/1‑136 (MQ=255)
                                                                                               aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGgcggcg                                                               <  2:151379/136‑1 (MQ=255)
                                                                                                     gagaACGCCAGAGGCGGTTATTTCGAGCATCTGACCCCGAACCCGACGCCGGCTCAGACCAG‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCATGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGa                                             <  6:231775/148‑1 (MQ=18)
                                                                                                     gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACATCTCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGa                                             >  1:271481/1‑149 (MQ=255)
                                                                                                     gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGAc                                            >  3:305617/1‑149 (MQ=255)
                                                                                                      agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGAcc                                           <  4:63171/149‑1 (MQ=255)
                                                                                                            ccACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAg                                      >  1:327181/1‑148 (MQ=255)
                                                                                                             cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGAGACCCTGAGc                                     >  1:252098/1‑148 (MQ=255)
                                                                                                                aGGCGGTAATTTCGAGCAACTGACCCCGAACCCGCCGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATc                                 >  1:322244/1‑149 (MQ=255)
                                                                                                                aGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATc                                 <  3:247854/149‑1 (MQ=255)
                                                                                                                  gCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCt                               <  4:305617/149‑1 (MQ=255)
                                                                                                                         tttCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAAt                                  >  5:187084/1‑139 (MQ=255)
                                                                                                                         tttCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAAt                                  <  6:187084/139‑1 (MQ=255)
                                                                                                                                  aCTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTaa               <  1:128602/149‑1 (MQ=255)
                                                                                                                                         ccGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAc        <  1:308775/149‑1 (MQ=255)
                                                                                                                                            aaCCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCtt     <  6:286044/149‑1 (MQ=255)
                                                                                                                                               ccGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTc  >  7:267025/1‑149 (MQ=255)
                                                                                                                                               ccGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTc  >  8:11979/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAC‑GATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTC  >  NC_002947/197547‑197838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑