Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,444,415	A→C	7.8%	*159G (TGA→GGA)	sulA ←	cell division inhibitor SulA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,444,415	0	A	C	7.8%	192.7 / 7.6	64	*159G (TGA→GGA)	sulA	cell division inhibitor SulA
Reads supporting (aligned to +/- strand):  ref base A (33/26);  new base C (3/2);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCG  >  NC_002947/2444272‑2444563                                                                                                                                                |                                                                                                                                                     cAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATTAGcc                                                                                                                                                   >  8:278763/1‑147 (MQ=255) cAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGcc                                                                                                                                                   >  8:313894/1‑147 (MQ=255) cAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGa                                                                                                                                                 <  2:212279/149‑1 (MQ=255) cAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTAGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGcc                                                                                                                                                   >  6:119725/1‑147 (MQ=255)      ccATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCgg                                                                                                                                            <  2:295146/149‑1 (MQ=255)          gCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGt                                                                                                                                        >  4:183378/1‑149 (MQ=255)           cTCAGTGTGGGCGTGAAGATGGCCGGTCTCCTGATGTATGGAGAGATCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGtt                                                                                                                                       >  4:162272/1‑149 (MQ=255)              aGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCa                                                                                                                                     <  5:119725/148‑1 (MQ=255)               gtgtGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAgg                                                                                                                                   >  8:12069/1‑149 (MQ=255)                   gggCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCttt                                                                                                                               >  7:180874/1‑149 (MQ=255)                       gTGCCGATGCCCGGTCGCCTGATGGATGGCGAGCTCTAGCGTGATGAAGCGCCGCGTGTGCTGGACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGc                                                                                                                           <  8:218678/149‑1 (MQ=255)                       gTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgtg                                                                                                                            >  8:12251/1‑148 (MQ=255)                         gCAGATGGCCGGTCGCCTTATGGATCGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGCCGGATGATCAGGCTTTGTGCAt                                                                                                                         >  2:147536/1‑149 (MQ=255)                           aGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCAttt                                                                                                                       >  1:294820/1‑149 (MQ=255)                              tGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCg                                                                                                                    <  6:203323/149‑1 (MQ=255)                                gCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCgg                                                                                                                   <  8:180874/148‑1 (MQ=255)                                       gCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCt                                                                                                           <  3:183378/149‑1 (MQ=255)                                                 tggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAg                                                                                                   >  7:90524/1‑147 (MQ=255)                                                 tggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAg                                                                                                   >  4:319960/1‑147 (MQ=255)                                                    cGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAgtt                                                                                              >  8:39378/1‑149 (MQ=255)                                                       gCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAgttgtt                                                                                           <  3:12172/149‑1 (MQ=255)                                                           gAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGGAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGc                                                                                        <  1:111236/148‑1 (MQ=255)                                                                tgatgaAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAg                                                                                                                                    >  6:339984/1‑99 (MQ=255)                                                                tgatgaAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAg                                                                                                                                    <  5:339984/99‑1 (MQ=255)                                                                       gggCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCAtt                                                                           <  3:319960/149‑1 (MQ=255)                                                                                   ctgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccag                                                               >  4:252551/1‑149 (MQ=255)                                                                                   ctgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccag                                                               >  3:298767/1‑149 (MQ=255)                                                                                   ctgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccag                                                               >  6:252688/1‑149 (MQ=255)                                                                                     gcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                              >  6:142550/1‑148 (MQ=255)                                                                                      cACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagcc                                                             <  3:162272/148‑1 (MQ=255)                                                                                       aCTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagccag                                                           >  3:189827/1‑149 (MQ=255)                                                                                             tgtgCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCt                                                         <  8:163303/145‑1 (MQ=255)                                                                                             tgtgCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCt                                                         >  7:163303/1‑145 (MQ=255)                                                                                                 cagcagCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACgg                                                 >  5:21861/1‑149 (MQ=255)                                                                                                  agcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCGAGCCAGCTCACCACGgt                                                >  8:274543/1‑149 (MQ=255)                                                                                                  agcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgt                                                <  7:52739/149‑1 (MQ=255)                                                                                                   gcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgtg                                               <  7:12251/149‑1 (MQ=255)                                                                                                    cagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTg                                                                    >  5:259076/1‑127 (MQ=255)                                                                                                    cagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTg                                                                    <  6:259076/127‑1 (MQ=255)                                                                                                    cagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgtgt                                              >  6:210203/1‑149 (MQ=255)                                                                                                     agcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGtgtg                                             >  3:93780/1‑149 (MQ=255)                                                                                                     agcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGtgtg                                             <  5:252688/149‑1 (MQ=255)                                                                                                     agcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGtgtg                                             <  3:252551/149‑1 (MQ=255)                                                                                                      gcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATTAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTgg                                            <  7:278763/149‑1 (MQ=255)                                                                                                         tATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTg                                         <  7:39378/149‑1 (MQ=255)                                                                                                               gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                              <  6:266185/122‑1 (MQ=255)                                                                                                               gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                              >  5:266185/1‑122 (MQ=255)                                                                                                               gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGcc                                    >  2:166058/1‑148 (MQ=255)                                                                                                                 tATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                                 >  6:9920/1‑149 (MQ=255)                                                                                                                   tttGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                                 <  6:21861/147‑1 (MQ=255)                                                                                                                       aaGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCTGGTGCa                            >  7:232110/1‑148 (MQ=255)                                                                                                                        aGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                          <  8:80881/149‑1 (MQ=255)                                                                                                                            aCAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                          >  4:259241/1‑145 (MQ=255)                                                                                                                            aCAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                          <  3:259241/145‑1 (MQ=255)                                                                                                                               ggCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTcac                   <  4:189827/149‑1 (MQ=255)                                                                                                                                gCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTcaca                  <  7:313894/149‑1 (MQ=255)                                                                                                                                gCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTca                    >  2:67958/1‑147 (MQ=255)                                                                                                                                     gCCTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGcc                       <  2:153648/139‑1 (MQ=255)                                                                                                                                     gCCTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGcc                       >  1:153648/1‑139 (MQ=255)                                                                                                                                      ccTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCCCGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAg            >  7:227660/1‑149 (MQ=39)                                                                                                                                        tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTgg                                            <  4:70475/115‑1 (MQ=39)                                                                                                                                        tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTgg                                            >  3:70475/1‑115 (MQ=39)                                                                                                                                        tGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt          >  6:69434/1‑149 (MQ=255)                                                                                                                                        tGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt          >  3:240904/1‑149 (MQ=255)                                                                                                                                                aGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAgcgcg  <  2:294820/149‑1 (MQ=255)                                                                                                                                                aGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGAAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAgcgcg  <  1:147536/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CAAGGCCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCG  >  NC_002947/2444272‑2444563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑