Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,444,424 G→T 7.8% R156S (CGC→AGC)  sulA ← cell division inhibitor SulA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,444,4240GT 7.8% 197.8 / 6.4 64R156S (CGC→AGC) sulAcell division inhibitor SulA
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base T (3/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

CCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCGCTTT  >  NC_002947/2444277‑2444567
                                                                                                                                                   |                                                                                                                                               
ccATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCgg                                                                                                                                                <  2:295146/149‑1 (MQ=255)
    gCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGt                                                                                                                                            >  4:183378/1‑149 (MQ=255)
     cTCAGTGTGGGCGTGAAGATGGCCGGTCTCCTGATGTATGGAGAGATCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGtt                                                                                                                                           >  4:162272/1‑149 (MQ=255)
        aGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCa                                                                                                                                         <  5:119725/148‑1 (MQ=255)
         gtgtGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAgg                                                                                                                                       >  8:12069/1‑149 (MQ=255)
             gggCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCttt                                                                                                                                   >  7:180874/1‑149 (MQ=255)
                 gTGCCGATGCCCGGTCGCCTGATGGATGGCGAGCTCTAGCGTGATGAAGCGCCGCGTGTGCTGGACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGc                                                                                                                               <  8:218678/149‑1 (MQ=255)
                 gTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgtg                                                                                                                                >  8:12251/1‑148 (MQ=255)
                   gCAGATGGCCGGTCGCCTTATGGATCGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGCCGGATGATCAGGCTTTGTGCAt                                                                                                                             >  2:147536/1‑149 (MQ=255)
                     aGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCAttt                                                                                                                           >  1:294820/1‑149 (MQ=255)
                        tGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCg                                                                                                                        <  6:203323/149‑1 (MQ=255)
                          gCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCgg                                                                                                                       <  8:180874/148‑1 (MQ=255)
                                 gCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCt                                                                                                               <  3:183378/149‑1 (MQ=255)
                                           tggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAg                                                                                                       >  4:319960/1‑147 (MQ=255)
                                           tggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAg                                                                                                       >  7:90524/1‑147 (MQ=255)
                                              cGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAgtt                                                                                                  >  8:39378/1‑149 (MQ=255)
                                                 gCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAgttgtt                                                                                               <  3:12172/149‑1 (MQ=255)
                                                     gAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGGAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGc                                                                                            <  1:111236/148‑1 (MQ=255)
                                                          tgatgaAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAg                                                                                                                                        >  6:339984/1‑99 (MQ=255)
                                                          tgatgaAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAg                                                                                                                                        <  5:339984/99‑1 (MQ=255)
                                                                 gggCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCAtt                                                                               <  3:319960/149‑1 (MQ=255)
                                                                             ctgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccag                                                                   >  3:298767/1‑149 (MQ=255)
                                                                             ctgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccag                                                                   >  6:252688/1‑149 (MQ=255)
                                                                             ctgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccag                                                                   >  4:252551/1‑149 (MQ=255)
                                                                               gcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                                  >  6:142550/1‑148 (MQ=255)
                                                                                cACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagcc                                                                 <  3:162272/148‑1 (MQ=255)
                                                                                 aCTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagccag                                                               >  3:189827/1‑149 (MQ=255)
                                                                                       tgtgCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCt                                                             <  8:163303/145‑1 (MQ=255)
                                                                                       tgtgCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCt                                                             >  7:163303/1‑145 (MQ=255)
                                                                                           cagcagCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACgg                                                     >  5:21861/1‑149 (MQ=255)
                                                                                            agcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCGAGCCAGCTCACCACGgt                                                    >  8:274543/1‑149 (MQ=255)
                                                                                            agcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgt                                                    <  7:52739/149‑1 (MQ=255)
                                                                                             gcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgtg                                                   <  7:12251/149‑1 (MQ=255)
                                                                                              cagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTg                                                                        >  5:259076/1‑127 (MQ=255)
                                                                                              cagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTg                                                                        <  6:259076/127‑1 (MQ=255)
                                                                                              cagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgtgt                                                  >  6:210203/1‑149 (MQ=255)
                                                                                               agcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGtgtg                                                 <  5:252688/149‑1 (MQ=255)
                                                                                               agcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGtgtg                                                 >  3:93780/1‑149 (MQ=255)
                                                                                               agcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGtgtg                                                 <  3:252551/149‑1 (MQ=255)
                                                                                                gcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATTAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTgg                                                <  7:278763/149‑1 (MQ=255)
                                                                                                   tATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTg                                             <  7:39378/149‑1 (MQ=255)
                                                                                                         gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                                  >  5:266185/1‑122 (MQ=255)
                                                                                                         gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                                  <  6:266185/122‑1 (MQ=255)
                                                                                                         gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGcc                                        >  2:166058/1‑148 (MQ=255)
                                                                                                           tATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                                     >  6:9920/1‑149 (MQ=255)
                                                                                                             tttGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                                     <  6:21861/147‑1 (MQ=255)
                                                                                                                 aaGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCTGGTGCa                                >  7:232110/1‑148 (MQ=255)
                                                                                                                  aGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                              <  8:80881/149‑1 (MQ=255)
                                                                                                                      aCAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                              >  4:259241/1‑145 (MQ=255)
                                                                                                                      aCAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                              <  3:259241/145‑1 (MQ=255)
                                                                                                                         ggCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTcac                       <  4:189827/149‑1 (MQ=255)
                                                                                                                          gCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTcaca                      <  7:313894/149‑1 (MQ=255)
                                                                                                                          gCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTca                        >  2:67958/1‑147 (MQ=255)
                                                                                                                               gCCTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGcc                           >  1:153648/1‑139 (MQ=255)
                                                                                                                               gCCTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGcc                           <  2:153648/139‑1 (MQ=255)
                                                                                                                                ccTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCCCGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAg                >  7:227660/1‑149 (MQ=39)
                                                                                                                                  tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTgg                                                <  4:70475/115‑1 (MQ=39)
                                                                                                                                  tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTgg                                                >  3:70475/1‑115 (MQ=39)
                                                                                                                                  tGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt              >  6:69434/1‑149 (MQ=255)
                                                                                                                                  tGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt              >  3:240904/1‑149 (MQ=255)
                                                                                                                                          aGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAgcgcg      <  2:294820/149‑1 (MQ=255)
                                                                                                                                          aGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGAAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAgcgcg      <  1:147536/149‑1 (MQ=255)
                                                                                                                                           gCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAgcgcgc     >  4:252005/1‑149 (MQ=255)
                                                                                                                                              gAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCGCttt  <  3:252005/149‑1 (MQ=255)
                                                                                                                                              gAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCGCttt  <  5:142550/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTTGCAGCGCGCTTT  >  NC_002947/2444277‑2444567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑