Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,469,860 T→C 6.3% intergenic (+29/+6) vacJ → / ← PP_2164 lipoprotein VacJ/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,469,8600TC 6.3% 180.9 / 2.5 63intergenic (+29/+6)vacJ/PP_2164lipoprotein VacJ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/30);  new base C (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

TCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGATCTGATCACCCAC  >  NC_002947/2469722‑2470006
                                                                                                                                          |                                                                                                                                                  
tctcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAAt                                                                                                                                          >  5:330369/1‑149 (MQ=255)
  tcGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAAt                                                                                                                                          <  7:234074/147‑1 (MQ=255)
    ggCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCAt                                                                                                                                      <  2:66769/149‑1 (MQ=255)
        gAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc                                                                                                                                   >  6:176298/1‑148 (MQ=255)
        gAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGc                                                                                                                                   >  4:253901/1‑148 (MQ=255)
             gCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAAt                                                                                                                             <  3:233313/149‑1 (MQ=255)
              cTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATc                                                                                                                            >  1:37970/1‑149 (MQ=255)
                   tCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCggg                                                                                                                                                <  4:23011/124‑1 (MQ=255)
                     aGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGAGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGcc                                                                                                                     >  7:200572/1‑149 (MQ=255)
                        gTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCg                                                                                                                   >  7:127472/1‑148 (MQ=255)
                               gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGc                                                                                                                         <  7:113101/135‑1 (MQ=255)
                               gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGc                                                                                                                         >  8:113101/1‑135 (MQ=255)
                               gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctg                                                                                                           >  6:297907/1‑149 (MQ=255)
                               gTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTctg                                                                                                           <  8:74147/149‑1 (MQ=255)
                                   aTCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCtgctgc                                                                                                       >  4:3264/1‑149 (MQ=255)
                                       tCATTCGCAATGCCTACCTTCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAATAGGCCTCGGGTTAAATGAATGGCAAGAATAAGCAGCCCGAAATTCTTCTgacctc                                                                                                   >  5:247997/1‑147 (MQ=255)
                                       tCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcccgc                                                                                                   >  2:335543/1‑149 (MQ=255)
                                          ttCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGc                                                                                                                         <  3:320727/124‑1 (MQ=255)
                                          ttCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGc                                                                                                                         >  4:320727/1‑124 (MQ=255)
                                              aaaTGCCTAACTGAAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTAGGGTTCAATGCATGGCCAGAATCCGCATCCCGAACTTCTGCTGCCCGCCCGGCTg                                                                                            <  6:247997/148‑1 (MQ=255)
                                               aaTGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTgg                                                                                           >  7:31748/1‑149 (MQ=255)
                                               aaTGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTgg                                                                                           >  1:329342/1‑149 (MQ=255)
                                                aTGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATGTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAGTGCATGGCCAGTATCCGCAGCCGGAACTTCTGCTGCCCGCCCGGCTGGt                                                                                          >  6:19101/1‑149 (MQ=255)
                                                aTGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCTGCTGGt                                                                                          <  2:301029/149‑1 (MQ=255)
                                                   ccTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCg                                                                                        <  6:330369/148‑1 (MQ=255)
                                                       ccTGCAAAACCGCGAGTTAAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCaa                                                                                    <  8:309506/148‑1 (MQ=255)
                                                             aaaCCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATAc                                                                             >  6:282897/1‑149 (MQ=255)
                                                              aaCCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCAGTGAACCAGGCGCCGCAAAGCGCCCTCGGGTTCAATGCATGGCAAGAATCCGCAGCCCGAACATCAGCTGCCCGCCCGGCTGGTCGGCAATCCATaa                                                                             >  7:166685/1‑147 (MQ=255)
                                                                        tCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGc                                                                  >  4:49153/1‑149 (MQ=255)
                                                                         cAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGTCCAGAATCCGCAGCCCGAACTTCTGCTTCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGc                                                                  <  2:37970/148‑1 (MQ=255)
                                                                          aaGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCt                                                                 <  7:68792/148‑1 (MQ=255)
                                                                          aaGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg                                                                <  3:3264/149‑1 (MQ=255)
                                                                            ggTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGa                                                                                                                               >  3:289516/1‑84 (MQ=255)
                                                                            ggTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGa                                                                                                                               <  4:289516/84‑1 (MQ=255)
                                                                             gTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATa                                                                              <  5:32737/132‑1 (MQ=255)
                                                                             gTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATa                                                                              >  6:32737/1‑132 (MQ=255)
                                                                                  ggATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg                                                                <  3:15466/141‑1 (MQ=255)
                                                                                  ggATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTg                                                                >  4:15466/1‑141 (MQ=255)
                                                                                     tGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAAc                                                                                                                >  5:159159/1‑90 (MQ=255)
                                                                                     tGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAAc                                                                                                                <  6:159159/90‑1 (MQ=255)
                                                                                       gCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGcccc                                                    <  8:31748/148‑1 (MQ=255)
                                                                                        cGAGGTCGAAGACGAGTTCTGATCTGTGAACGTGGGGCCGCATAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGGCCGAACTTGTGCTGCCCGCGCGGCTGGGCGGGAATCCATACCTCTTCGGTGCTGGCATGCAGCCCCtt                                                  <  8:166685/149‑1 (MQ=255)
                                                                                           ggTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAg                                               >  5:298319/1‑149 (MQ=255)
                                                                                           ggTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAg                                               <  3:253901/149‑1 (MQ=255)
                                                                                                 aGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCg                                          >  3:323169/1‑148 (MQ=255)
                                                                                                  gacgacTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGa                                        <  5:282897/149‑1 (MQ=255)
                                                                                                          cTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCTAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCa                                                                                >  2:128518/1‑101 (MQ=255)
                                                                                                          cTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCTAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCa                                                                                <  1:128518/101‑1 (MQ=255)
                                                                                                             tgcattgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTc                                                 >  2:261197/6‑129 (MQ=37)
                                                                                                             tgcattgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTc                                                 <  1:261197/124‑1 (MQ=37)
                                                                                                             tgcattgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTc                             <  2:180534/144‑1 (MQ=38)
                                                                                                             aTCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcccgc                                                                                                   <  1:248943/79‑1 (MQ=255)
                                                                                                             aTCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTgcccgc                                                                                                   >  2:248943/1‑79 (MQ=255)
                                                                                                             aTCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGa                                <  5:297907/146‑1 (MQ=255)
                                                                                                                attgAACCCGAGGCCGCTTTGCGGCCCCAGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGAt                          >  7:119783/3‑149 (MQ=38)
                                                                                                                  tgAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGa                           <  6:298319/146‑1 (MQ=255)
                                                                                                                    aaCCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCg                       >  4:163645/1‑148 (MQ=255)
                                                                                                                       cTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGa                                <  6:190409/136‑1 (MQ=255)
                                                                                                                       cTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGa                                >  5:190409/1‑136 (MQ=255)
                                                                                                                        tGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACg                                  <  6:261471/133‑1 (MQ=255)
                                                                                                                        tGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACg                                  >  5:261471/1‑133 (MQ=255)
                                                                                                                             ccGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGATc             <  5:176298/149‑1 (MQ=255)
                                                                                                                                        ccTCGGGTTCATTGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTTGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGATCTGATcacccac  <  5:19101/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
TCTCGGCCGAAAAGCTGATTCAGGGTGACAAGTACATCTTCATTCGCAATGCCTACCTGCAAAACCGCGAGTTCAAGGTGAAGGATGGCGAGGTCGAAGACGACTTCTGATCTGTGAACCTGGGGCCGCAAAGCGGCCTCGGGTTCAATGCATGGCCAGAATCCGCAGCCCGAACTTCTGCTGCCCGCCCGGCTGGTCGGCAATCCATACCACTTCGGTGCTGGCATGCAGCCCCTTCAGCGCCGGATGGACGGAGTCGATCCGTACCTCGATCTGATCACCCAC  >  NC_002947/2469722‑2470006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑