Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,482,504 T→A 6.7% intergenic (‑129/‑49) PP_2176 ← / → PP_2177 transcriptional regulator, LysR family/transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,482,5040TA 6.7% 223.6 / 5.0 74intergenic (‑129/‑49)PP_2176/PP_2177transcriptional regulator, LysR family/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base A (3/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

TGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCAT  >  NC_002947/2482360‑2482647
                                                                                                                                                |                                                                                                                                               
tGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAt                                                                                                                                             >  5:3895/1‑149 (MQ=255)
tGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAt                                                                                                                                             >  2:212222/1‑149 (MQ=255)
tGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGAAAAt                                                                                                                                             >  7:297869/1‑149 (MQ=255)
   aggaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAtttt                                                                                                                                          <  7:294108/149‑1 (MQ=255)
    ggaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTc                                                                                                                                         <  7:39079/149‑1 (MQ=255)
    ggaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTCACGCGTGAAATTTTTCATGTAAATTTTc                                                                                                                                         <  1:193749/149‑1 (MQ=255)
     gaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCa                                                                                                                                        <  3:311955/149‑1 (MQ=255)
     gaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCa                                                                                                                                        >  7:76111/1‑149 (MQ=255)
          aGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGaa                                                                                                                                    >  2:325918/1‑148 (MQ=255)
             cACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAAt                                                                                                                                <  8:76111/149‑1 (MQ=255)
                  ggCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGAAAATTTACATGAAAAAtttcac                                                                                                                           <  8:297869/149‑6 (MQ=255)
                         cGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAAt                                                                                                                    >  2:297911/1‑149 (MQ=255)
                          gCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGTGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAAtt                                                                                                                   >  3:249101/1‑149 (MQ=255)
                              aGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCAc                                                                                                               <  3:124521/149‑1 (MQ=255)
                              aGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCAc                                                                                                               >  5:115154/1‑149 (MQ=255)
                               gcgcCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACg                                                                                                              <  1:225561/149‑1 (MQ=255)
                                  catgaccaGAAACCCGCGCCACCGCAGCCAATCCGCCCCGTTGCGCATCAAGACCTGTGTGGGGGGCTTCACATTTCCATCGTTCGTCTTGTCGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCa                                                                                                           <  4:249101/142‑1 (MQ=255)
                                      tAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTcc                                                                                                       >  5:27841/1‑149 (MQ=255)
                                      tAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTcc                                                                                                       <  7:45716/149‑1 (MQ=255)
                                         aGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGa                                                                                                     >  7:22630/1‑148 (MQ=255)
                                         aGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGa                                                                                                     >  1:224134/1‑148 (MQ=255)
                                            aaCCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTATATTTACATGAAACATAAGTCTAAAAATTTCACGGCAGTCCGAAAc                                                                                                  >  8:146200/1‑148 (MQ=255)
                                             aCCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGa                                                                                                <  6:27841/149‑1 (MQ=255)
                                               ccGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGAt                                                                                               >  1:139630/1‑148 (MQ=255)
                                               ccGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATg                                                                                              <  8:128316/149‑1 (MQ=255)
                                                cgcgCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGa                                                                                             <  5:305104/149‑1 (MQ=255)
                                                 gcgcCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATg                                                                                              >  6:136331/1‑147 (MQ=255)
                                                 gcgcCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATg                                                                                              >  2:118043/1‑147 (MQ=255)
                                                   gcCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATg                                                                                              >  4:216652/1‑145 (MQ=255)
                                                       ccGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAAt                                                                                                                                <  3:258849/107‑1 (MQ=255)
                                                       ccGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAAt                                                                                                                                >  4:258849/1‑107 (MQ=255)
                                                        cGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCAc                                                                                     <  6:115154/149‑1 (MQ=255)
                                                            gCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAag                                                                                 <  1:297911/149‑1 (MQ=255)
                                                             ccAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGAAAATTTACATGAAAAAtttcac                                                                                                                           >  7:268738/1‑101 (MQ=38)
                                                             ccAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGAAAATTTACATGAAAAAtttcac                                                                                                                           <  8:268738/106‑6 (MQ=38)
                                                              cAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAagag                                                                               >  3:47257/1‑149 (MQ=255)
                                                               aaTCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAgg                                                                              >  3:41285/1‑149 (MQ=255)
                                                                aTCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGt                                                                             >  4:152209/1‑149 (MQ=255)
                                                                aTCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGt                                                                             >  3:71434/1‑149 (MQ=255)
                                                                 tCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGtt                                                                            <  6:3895/149‑1 (MQ=255)
                                                                  ccGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAATTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTg                                                                           <  5:59797/149‑1 (MQ=255)
                                                                    gCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGaa                                                                         <  1:212222/149‑1 (MQ=255)
                                                                     ccACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAg                                                                        <  3:155276/149‑1 (MQ=255)
                                                                         gTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACg                                                                                                              <  6:296304/107‑1 (MQ=255)
                                                                         gTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACg                                                                                                              >  5:296304/1‑107 (MQ=255)
                                                                                    aGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTa                                                         <  2:139630/149‑1 (MQ=255)
                                                                                     gCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCa                                                                                                           >  8:20816/1‑98 (MQ=255)
                                                                                     gCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCa                                                                                                           <  7:20816/98‑1 (MQ=255)
                                                                                     gCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAgg                                                                              >  5:252401/1‑127 (MQ=255)
                                                                                     gCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTAt                                                        >  4:76742/1‑149 (MQ=255)
                                                                                     gCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTAt                                                        <  3:216652/149‑1 (MQ=255)
                                                                                      cccTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTAtt                                                       <  1:325918/149‑1 (MQ=255)
                                                                                        ctctGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGACGAAGATGCAAGAAGAGGTTGAAGCCCTCGCGATCCTTATTCa                                                     >  8:47362/1‑149 (MQ=255)
                                                                                              gggCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTAt                                                        <  4:231210/140‑1 (MQ=255)
                                                                                              gggCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTAt                                                        >  3:231210/1‑140 (MQ=255)
                                                                                               ggcggcTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTg                                              >  4:283720/1‑149 (MQ=255)
                                                                                                         caTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCa                                    <  8:22630/149‑1 (MQ=255)
                                                                                                          aTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTa                                                         <  6:50270/127‑1 (MQ=255)
                                                                                                          aTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGAGGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTc                                     >  4:56229/1‑147 (MQ=255)
                                                                                                                 tCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTg                            <  3:152209/149‑1 (MQ=255)
                                                                                                                   gTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGTACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACATGAc                          >  1:166396/1‑149 (MQ=255)
                                                                                                                    ttCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGAcc                         >  3:17664/1‑149 (MQ=255)
                                                                                                                        gCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTgg                     <  3:76742/149‑1 (MQ=255)
                                                                                                                         cTTGACGCGTGAAATTTTTCATGTAAATTTACATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTggg                    >  2:236117/1‑149 (MQ=255)
                                                                                                                            gACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTg                  >  7:127079/1‑148 (MQ=255)
                                                                                                                              cgcgTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAAc               <  6:101076/149‑1 (MQ=255)
                                                                                                                               gcgTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGAt                                                              <  4:8982/101‑1 (MQ=255)
                                                                                                                                cgTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTg                      >  7:195084/1‑140 (MQ=255)
                                                                                                                                cgTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTg                      <  8:190081/140‑1 (MQ=255)
                                                                                                                                cgTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTg                      <  8:195084/140‑1 (MQ=255)
                                                                                                                                  tGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGc           >  3:284652/1‑149 (MQ=255)
                                                                                                                                    ttaTTTTTCATGAAAATTTACATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGcc          >  5:215934/3‑148 (MQ=255)
                                                                                                                                           tCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCa   <  1:300867/148‑1 (MQ=255)
                                                                                                                                            cATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCAt  <  4:71434/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCAT  >  NC_002947/2482360‑2482647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑