Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,518,456 C→G 9.5% L292L (CTC→CTG)  PP_2211 → AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,518,4560CG 9.5% 127.6 / 4.4 42L292L (CTC→CTG) PP_2211AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (15/23);  new base G (2/2);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

CCCTGAGCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAGGGGCAGGGCAC  >  NC_002947/2518312‑2518600
                                                                                                                                                |                                                                                                                                                
cccTGAGCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGcc                                                                                                                                              <  1:168724/149‑1 (MQ=255)
cccTGAGCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGCCTCAGc                                                                                                                                               >  7:290770/1‑148 (MQ=255)
  cTGAGCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAg                                                                                                                                            <  4:30893/149‑1 (MQ=255)
  cTGAGCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAg                                                                                                                                            <  3:234826/149‑1 (MQ=255)
      gCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTGAGCCAggcga                                                                                                                                        <  4:1767/149‑4 (MQ=255)
      gCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTGAGCCAggcga                                                                                                                                        <  1:87416/149‑4 (MQ=255)
      gCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAgg                                                                                                                                           >  4:268788/1‑146 (MQ=255)
            gCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCTGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTg                                                                                                                                   <  7:312656/148‑1 (MQ=255)
              ccGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGc                                                                                                                                 >  7:162215/1‑148 (MQ=255)
                   gCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATggg                                                                                                                           >  3:128665/1‑149 (MQ=255)
                          gCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCtt                                                                                                                    <  4:128665/149‑1 (MQ=255)
                            cTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGc                                                                                                                  <  8:162215/149‑1 (MQ=255)
                                 tCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGa                                                                                                                        <  7:315514/138‑1 (MQ=255)
                                 tCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGa                                                                                                                        >  8:315514/1‑138 (MQ=255)
                                             ccTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTc                                                                                                  <  2:14109/148‑1 (MQ=255)
                                             ccTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGGTTGGGGTTCg                                                                                                 >  7:27993/1‑149 (MQ=255)
                                                   gCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGa                                                                                             >  1:49213/1‑147 (MQ=255)
                                                   gCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGAca                                                                                           <  1:81757/149‑1 (MQ=255)
                                                            ccTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGt                                                                                  >  6:247050/1‑149 (MQ=255)
                                                             cTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGtt                                                                                 <  3:268788/149‑1 (MQ=255)
                                                                cGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCt                                                                               <  6:216346/148‑1 (MQ=255)
                                                                cGTCGGAGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCTTTTGGGGTTCGCCGACAGCAGCTCGTTc                                                                                <  5:206283/147‑1 (MQ=255)
                                                                 gTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCtat                                                                             <  2:49213/149‑1 (MQ=255)
                                                                     gcgcCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGAGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCAATGGGAGCGCTTGCCGAGCGTTTGGGTTTCGCAGACAGCAGCTCGGTCTATAAg                                                                          >  6:330051/1‑148 (MQ=255)
                                                                            gCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCg                                                                  >  3:308392/1‑149 (MQ=255)
                                                                                  gagGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTg                                                            >  2:284242/1‑149 (MQ=255)
                                                                                       gCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTc                                                        >  6:335293/1‑148 (MQ=255)
                                                                                       gCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTc                                                        <  5:335293/148‑1 (MQ=255)
                                                                                                 cAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAAccc                                             >  5:318100/1‑149 (MQ=255)
                                                                                                   gggCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCgg                                           >  6:22253/1‑149 (MQ=255)
                                                                                                    ggCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCggg                                          <  4:308392/149‑1 (MQ=255)
                                                                                                          aaGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGGCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACAtt                                     <  8:126266/148‑1 (MQ=255)
                                                                                                            ggACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATc                                  <  5:56979/149‑1 (MQ=255)
                                                                                                                aGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCggg                               >  3:267994/1‑148 (MQ=255)
                                                                                                                 gCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCa                             <  5:247050/149‑1 (MQ=255)
                                                                                                                          gggAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAg                    <  4:267994/149‑1 (MQ=255)
                                                                                                                                  acgtcttccTGGCTGAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAgg            >  3:19731/8‑149 (MQ=255)
                                                                                                                                       ttccTGGCTGAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAGgggcag       >  3:93577/3‑149 (MQ=255)
                                                                                                                                        gCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAGgggcagg      <  3:81259/149‑1 (MQ=255)
                                                                                                                                        gCCTGGCGCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAGgggcag       <  8:230608/148‑1 (MQ=255)
                                                                                                                                             gCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGGTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAGgggcagggca   <  7:235214/147‑1 (MQ=255)
                                                                                                                                             gCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATATCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAGGGGCAGGGCAc  <  8:27993/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCCTGAGCAATGGCCCGATGCTGACCGCCTGGCTCGGCAGCTGTGCCTGTCGCCATCGACCCTGCGTCGGCGCCTGGCAGAAGAGGGGCAGAACTACCAGGGCTTGAAGGACAGCGTACGACGGGAGCTGGCGATCGCCTGGCTCAGCCAGGAAGACGTGGCCATGGGCGAGCTTGCCGAGCGTTTGGGGTTCGCCGACAGCAGCTCGTTCTATAAGGCATTTCGCAAGTGGTTCGGCTGCAACCCGGGACATTATCGGGCATTGATCCAGGCGCGAGGGGCAGGGCAC  >  NC_002947/2518312‑2518600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑