Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,573,549 T→C 11.5% I102T (ATC→ACC)  PP_2257 → aerotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,573,5490TC11.5% 132.0 / 8.1 52I102T (ATC→ACC) PP_2257aerotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (24/22);  new base C (2/4);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

CCTGGTGCGCCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTG  >  NC_002947/2573406‑2573691
                                                                                                                                               |                                                                                                                                              
ccTGGTGCGCCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTc                                                                                                                                            >  6:175053/1‑148 (MQ=255)
    gTGCGCCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGaa                                                                                                                                         <  7:238330/147‑1 (MQ=255)
       cgcCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTgg                                                                                                                                    <  2:199836/149‑1 (MQ=255)
        gcCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCACCTTCGaggat                                                                                                                                      >  5:295691/1‑142 (MQ=255)
        gcCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCACCTTCGaggat                                                                                                                                      <  6:295691/146‑5 (MQ=255)
         ccACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCACCTTCGAGGATGGGc                                                                                                                                  <  8:305966/149‑1 (MQ=255)
           aCCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCACCTTCGAGGATGGGCgt                                                                                                                                <  7:132786/149‑2 (MQ=255)
              cGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTa                                                                                                                             <  5:175053/149‑1 (MQ=255)
                    tGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGt                                                                                                                       <  6:309337/149‑1 (MQ=255)
                     accgccgGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGt                                                                                                                       >  7:217836/2‑148 (MQ=255)
                      ccgccgGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTa                                                                                                                     >  8:201664/1‑149 (MQ=255)
                       cgccgGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTAc                                                                                                                    >  5:193574/1‑149 (MQ=255)
                              ggTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCgg                                                                                                             >  7:240260/1‑149 (MQ=255)
                               gtgtACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGt                                                                                                            >  2:339027/1‑149 (MQ=255)
                                    cGAGTTGATGTGGCGCTACCTGAAAGCCTGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTgcgc                                                                                                        <  4:95108/148‑1 (MQ=255)
                                    cGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTgcgc                                                                                                        <  1:339027/148‑1 (MQ=255)
                                                      ccTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGa                                                                                       <  8:70652/147‑1 (MQ=255)
                                                      ccTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGa                                                                                       <  2:296214/147‑1 (MQ=255)
                                                        tGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTAcgc                                                                                           <  7:300242/141‑1 (MQ=255)
                                                        tGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTAcgc                                                                                           >  8:300242/1‑141 (MQ=255)
                                                         gAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGt                                                                                                            >  4:239780/1‑123 (MQ=255)
                                                         gAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGt                                                                                                            <  3:239780/123‑1 (MQ=255)
                                                            aGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAg                                                                               <  6:193574/149‑1 (MQ=255)
                                                            aGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTAGGGCATAcgcgcac                                                                                       <  5:141031/141‑3 (MQ=255)
                                                            aGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTAGGGCATAcgcgcac                                                                                       >  6:141031/1‑139 (MQ=255)
                                                                           gATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGccc                                                                >  7:1500/1‑149 (MQ=255)
                                                                            aTGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCt                                                               <  3:182180/149‑1 (MQ=255)
                                                                               ggTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGt                                                             <  5:113006/148‑1 (MQ=255)
                                                                                gTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTAt                                                           <  1:91292/149‑1 (MQ=255)
                                                                                gTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTAt                                                           >  3:268893/1‑149 (MQ=255)
                                                                                      tCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCgg                                                     >  6:59462/1‑149 (MQ=255)
                                                                                      tCAAGAACCGCTGCCGTAACGGAGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCgg                                                     <  8:217836/149‑1 (MQ=255)
                                                                                         aGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGc                                                                        >  6:16973/1‑127 (MQ=255)
                                                                                         aGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGc                                                                        <  5:16973/127‑1 (MQ=255)
                                                                                          gAACCGCTGCGGTAACTGAGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTgc                                                 <  4:176117/149‑1 (MQ=255)
                                                                                                   ccGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACgg                                          <  8:240260/147‑1 (MQ=255)
                                                                                                             gACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCAtt                              <  7:201664/149‑1 (MQ=255)
                                                                                                              actactactGGGTCAATGCCTACGTCACGCGCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTg                             >  1:27460/1‑149 (MQ=255)
                                                                                                                tactactGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTc                           >  3:10026/1‑149 (MQ=255)
                                                                                                                  ctactGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCgg                         >  8:164092/1‑149 (MQ=255)
                                                                                                                      tGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGGGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGgcgcc                     >  1:21834/1‑149 (MQ=255)
                                                                                                                          tCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTg                             >  7:151786/1‑137 (MQ=255)
                                                                                                                          tCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTg                             <  8:151786/137‑1 (MQ=255)
                                                                                                                          tCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGgcgccgcgc                 >  5:146226/1‑149 (MQ=255)
                                                                                                                          tCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGgcgccgcgc                 >  3:246289/1‑149 (MQ=255)
                                                                                                                              tGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGc             >  6:154645/1‑149 (MQ=255)
                                                                                                                                 cTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTc                           <  7:173600/132‑1 (MQ=255)
                                                                                                                                 cTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTc                           >  8:173600/1‑132 (MQ=255)
                                                                                                                                 cTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCt            >  6:131039/1‑147 (MQ=255)
                                                                                                                                       cccGCCCACCTTCGAGGATGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCt                                                               >  4:144522/3‑90 (MQ=38)
                                                                                                                                       cccGCCCACCTTCGAGGATGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCt                                                               <  3:144522/88‑1 (MQ=38)
                                                                                                                                         cGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTg  >  8:305957/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CCTGGTGCGCCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTG  >  NC_002947/2573406‑2573691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑