Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,696,295 C→G 5.7% L66V (CTC→GTC)  PP_2363 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,696,2950CG 5.7% 226.3 / 3.9 70L66V (CTC→GTC) PP_2363hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/33);  new base G (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

GCAGTACGGCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTACGACTTC  >  NC_002947/2696149‑2696438
                                                                                                                                                    |                                                                                                                                               
gCAGTACGGCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTc                                                                                                                                               <  2:252798/149‑1 (MQ=255)
  aGTACGGCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTcag                                                                                                                                             >  2:295115/1‑149 (MQ=255)
   gTACGGCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTcagc                                                                                                                                            <  7:307571/149‑1 (MQ=255)
    tACGGCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTcag                                                                                                                                             >  7:75527/1‑147 (MQ=255)
        gCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCTGGTTCGCAACACGGTACAGACGGCTTCCAGGCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCgg                                                                                                                                       <  7:179294/149‑1 (MQ=255)
        gCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCgg                                                                                                                                       >  1:152070/1‑149 (MQ=255)
          cTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTg                                                                                                                                     <  8:212493/149‑1 (MQ=255)
             ggCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATc                                                                                                                                  <  4:234721/149‑1 (MQ=255)
                  tttGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGc                                                                                                                                                 <  1:19069/129‑1 (MQ=255)
                  tttGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGc                                                                                                                                                 >  2:19069/1‑129 (MQ=255)
                      ttCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTAc                                                                                                                          <  4:307774/148‑1 (MQ=255)
                         gtcgGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTC‑C‑GCTGCTGAGCAAGGTCAGCAGCgaa                                                                                                                                      <  5:300325/133‑3 (MQ=255)
                         gtcgGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTC‑C‑GCTGCTGAGCAAGGTCAGCAGCgaa                                                                                                                                      >  6:300325/1‑131 (MQ=255)
                           cgGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCAc                                                                                                                     >  2:263261/1‑148 (MQ=255)
                           cgGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCAc                                                                                                                     >  2:259007/1‑148 (MQ=255)
                              tGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATc                                                                                                                 <  8:75527/149‑1 (MQ=255)
                                 ccACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCTACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCAcg                                                                                                              <  7:288609/149‑1 (MQ=255)
                                  cACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCAACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCAcgc                                                                                                             >  1:46877/1‑149 (MQ=255)
                                         ccGGCAGCGTTTGGCATCCTTTATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑CTGCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCAcgcccgcc                                                                                                        <  1:259007/148‑1 (MQ=255)
                                         ccGGCAGCGTTTGGCATCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTTCC‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCAcgcccgcc                                                                                                        <  1:263261/148‑1 (MQ=255)
                                          cGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCg                                                                                                     >  3:119470/1‑149 (MQ=255)
                                            gCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCggg                                                                                                   >  6:255063/1‑149 (MQ=255)
                                              aGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCgg                                                                                                                                       <  1:124177/111‑1 (MQ=255)
                                              aGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCgg                                                                                                                                       >  2:124177/1‑111 (MQ=255)
                                                    tGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTgg                                                                                           >  6:94196/1‑149 (MQ=255)
                                                    tGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTgg                                                                                           >  8:144314/1‑149 (MQ=255)
                                                      gCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTc                                                                                         <  1:295115/149‑1 (MQ=255)
                                                         gCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGc                                                                                      <  2:46877/149‑1 (MQ=255)
                                                          ccTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGcc                                                                                     >  7:138444/1‑149 (MQ=255)
                                                           cTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCgactg                                                                                    >  1:121578/1‑145 (MQ=255)
                                                           cTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGccc                                                                                    <  6:137726/149‑1 (MQ=255)
                                                           cTTAATTCGACGCAGGTTCGCAACACCGTACAGACGGCTTCCAGCATCAACACCGGATTGCAGTCCACCGGTT‑C‑GCTGATGACCTAACTCAGCAGCGGTGATAATTTCTACGCCACGATCACCCACGCCACCGGTCGGCTGGTCGGccc                                                                                    >  5:114758/1‑149 (MQ=255)
                                                                ttCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAg                                                                               >  5:8685/1‑149 (MQ=255)
                                                                      gCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGcc                                                                                     >  6:340217/1‑137 (MQ=255)
                                                                      gCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGcc                                                                                     <  5:340217/137‑1 (MQ=255)
                                                                      gCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGAT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTCGGCTGGTCGGCCCGACAGGCGATg                                                                         >  5:34164/1‑149 (MQ=255)
                                                                        aGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGt                                                                        >  2:27333/1‑148 (MQ=255)
                                                                           ttCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCg                                                                                   >  8:96392/1‑134 (MQ=255)
                                                                           ttCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCg                                                                                   <  7:96392/134‑1 (MQ=255)
                                                                           ttCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCAtt                                                                    <  8:138444/149‑1 (MQ=255)
                                                                               cAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGCT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCt                                                                <  1:27333/149‑1 (MQ=255)
                                                                                aaCACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTa                                                               >  3:176835/1‑149 (MQ=255)
                                                                                    cGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACg                                                           <  2:152070/149‑1 (MQ=255)
                                                                                      gTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCt                                                         <  8:42366/149‑1 (MQ=255)
                                                                                         cAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGt                                                       <  4:119470/148‑1 (MQ=255)
                                                                                            aCGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTAt                                                     <  8:112549/147‑1 (MQ=255)
                                                                                            aCGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTAt                                                     >  7:112549/1‑147 (MQ=255)
                                                                                             cGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGc                                                   <  5:19143/148‑1 (MQ=255)
                                                                                             cGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGc                                                   >  6:19143/1‑148 (MQ=255)
                                                                                                cTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGa                                                >  2:49849/1‑148 (MQ=255)
                                                                                                 ttCCAGCCTCCACTCCGGGTTGCCGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGTGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACTGGCGATGGCATTCCCTACACGCTGTATGCCGac                                               >  5:291294/1‑148 (MQ=255)
                                                                                                 ttCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GATGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGac                                               >  8:20266/1‑148 (MQ=255)
                                                                                                    cAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGacaac                                            <  1:49849/148‑1 (MQ=255)
                                                                                                         tCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACaccacc                                      >  3:165292/1‑149 (MQ=255)
                                                                                                                cGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTAcc                               <  4:176835/149‑1 (MQ=255)
                                                                                                                 gggTTGCAGTGCACAGGTT‑C‑GCTGCTGACCTTACTCAGCAGCGGTGATCATTTCTACTCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTAccc                              <  6:114758/149‑1 (MQ=255)
                                                                                                                   gTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGa                            <  5:255063/149‑1 (MQ=255)
                                                                                                                        aGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTAcc                       >  6:45441/1‑149 (MQ=255)
                                                                                                                            cACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTAGACGCTGTATGCCGACAACACCACCCGCTACCCGATTTCCCgg                    <  5:235717/148‑1 (MQ=255)
                                                                                                                            cACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCggg                   >  3:295110/1‑149 (MQ=255)
                                                                                                                             aCCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCgggg                  >  6:339345/1‑149 (MQ=255)
                                                                                                                                 gTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGtgtg              <  5:339345/149‑1 (MQ=255)
                                                                                                                                  tt‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTgg             <  6:8685/149‑1 (MQ=255)
                                                                                                                                   t‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGc            >  7:165726/1‑149 (MQ=255)
                                                                                                                                     c‑GCTGCTGAGCAAGGTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTggg                                                                                               >  2:180897/1‑65 (MQ=37)
                                                                                                                                     c‑GCTGCTGAGCAAGGTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTggg                                                                                               <  1:180897/65‑1 (MQ=37)
                                                                                                                                         tgctgACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTAc        >  6:102502/1‑149 (MQ=255)
                                                                                                                                           ctgACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTACGa      <  6:34164/149‑1 (MQ=255)
                                                                                                                                             gACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTACGACt    >  3:143032/1‑149 (MQ=255)
                                                                                                                                               ccTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTACGACTTc  <  7:144314/149‑1 (MQ=255)
                                                                                                                                                cTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTAc        <  8:131159/142‑1 (MQ=255)
                                                                                                                                                cTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTAc        >  7:131159/1‑142 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCAGTACGGCCTGGGCGCTTTGTTCGTCGGTGGCCACCTTGCCGGCAGCGTTTGGCAGCCTTAATTCGACGCAGGTTCGCAACACGGTACAGACGGCTTCCAGCCTCAACTCCGGGTTGCAGTGCACCGGTT‑C‑GCTGCTGACCTTGCTCAGCAGCGGTGATCATTTCTACGCCACGATCACGCCCGCCTCGGGTGGGCTGGTCGGCCCGACAGGCGATGTCATTCCCTACACGCTGTATGCCGACAACACCACCCGCTACCCGATTACCCGGGGTGTGGCCTACGACTTC  >  NC_002947/2696149‑2696438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑