Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,709,592 G→A 7.4% V57V (GTG→GTA)  PP_2372 → sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,709,5920GA 7.4% 155.0 / 2.3 54V57V (GTG→GTA) PP_2372sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

GCCTTGTCTTGCCACTGGTGGCAAATGCGCAACCGGCTTCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCGGGGTGAA  >  NC_002947/2709450‑2709738
                                                                                                                                                 |                                                                                                                                                  
gCCTTGTCTTGCCACTGGTGGCAAATGCGCAACCGGCTTCGGTGGTGTTTCTCAACCCGGGGCTGTATGGCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCaa                                                                                                                                                 >  1:102575/1‑149 (MQ=255)
 ccTTGTCTTGCCACTGGTGGCAAATGCGCAACCGGCTTCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAAtata                                                                                                                                             <  8:232108/149‑1 (MQ=255)
     gTCTTGCCACTGGTGGCAAATGCGCAACCGGCTTCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATagcga                                                                                                                                         >  3:169068/1‑149 (MQ=255)
                              aaCCGGCTTCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAAtata                                                                                                                                             >  4:100009/1‑120 (MQ=255)
                              aaCCGGCTTCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAAtata                                                                                                                                             <  3:100009/120‑1 (MQ=255)
                                  ggCTTCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAgg                                                                                                            >  4:63439/1‑149 (MQ=255)
                                     ttCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGccc                                                                                                         >  8:34258/1‑149 (MQ=255)
                                       cGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGccc                                                                                                         <  4:182585/147‑1 (MQ=255)
                                                  ctcAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAg                                                                                            >  5:265644/1‑149 (MQ=255)
                                                   tcAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAgc                                                                                           >  7:43332/1‑149 (MQ=255)
                                                   tcAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAgc                                                                                           >  2:183661/1‑149 (MQ=255)
                                                     aaCCCGGGGCTGT‑‑‑CCCCCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTACAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAgcgc                                                                                         >  3:75743/1‑149 (MQ=255)
                                                     aaCCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACTTGACACTGACCCAGGCCCGGGCGATCCTGAgc                                                                                           <  1:40074/147‑1 (MQ=255)
                                                     aaCCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATc                                                                                                 <  7:146439/141‑1 (MQ=255)
                                                     aaCCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATc                                                                                                 >  8:146439/1‑141 (MQ=255)
                                                     aaCCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAgcgc                                                                                         <  4:166314/149‑1 (MQ=255)
                                                     aaCCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTACAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAgcgc                                                                                         >  3:71228/1‑149 (MQ=255)
                                                             gCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGc                                                                                 >  5:144394/1‑149 (MQ=255)
                                                                     ccACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCg                                                                            >  1:121230/1‑149 (MQ=255)
                                                                      cACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCg                                                                            >  5:168339/1‑148 (MQ=255)
                                                                               aTTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTgg                                                                  >  3:258996/1‑149 (MQ=255)
                                                                               aTTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTgg                                                                  <  6:265644/149‑1 (MQ=255)
                                                                                  cTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTg                                                                <  6:144394/148‑1 (MQ=255)
                                                                                   tGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCt                                                              >  3:325422/1‑149 (MQ=255)
                                                                                     gACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTg                                                             <  7:34258/148‑1 (MQ=255)
                                                                                     gACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTACAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTgg                                                            <  4:75743/149‑1 (MQ=255)
                                                                                     gACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTACAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTgg                                                            <  4:71228/149‑1 (MQ=255)
                                                                                       ccAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAgcgccgc                                                                                                                                  <  5:190085/77‑1 (MQ=255)
                                                                                       ccAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAgcgccgc                                                                                                                                  >  6:190085/1‑77 (MQ=255)
                                                                                          gCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAAc                                                       >  8:135048/1‑149 (MQ=255)
                                                                                                cccGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAAc                                                       <  1:203341/143‑1 (MQ=255)
                                                                                                cccGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAAc                                                       >  2:203341/1‑143 (MQ=255)
                                                                                                 ccGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGCCCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAg                                                 >  5:11855/1‑148 (MQ=255)
                                                                                                 ccGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAg                                                 >  4:28427/1‑148 (MQ=255)
                                                                                                 ccGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAg                                                 >  5:342566/1‑148 (MQ=255)
                                                                                                 ccGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAg                                                 >  6:71072/1‑148 (MQ=255)
                                                                                                        aTGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTgg                                          <  4:169068/148‑1 (MQ=255)
                                                                                                          gCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTgg                                          >  1:238164/1‑146 (MQ=255)
                                                                                                          gCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTgg                                          <  2:238164/146‑1 (MQ=255)
                                                                                                            aGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCg                                     >  8:242395/1‑149 (MQ=255)
                                                                                                                cgccgcGGACTAGCTGGGCATGACGCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGaaa                                 <  5:47113/149‑1 (MQ=255)
                                                                                                                cgccgcGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGaaa                                 <  1:285814/149‑1 (MQ=255)
                                                                                                                    gcgGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCt                             <  2:102575/149‑1 (MQ=255)
                                                                                                                             cTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTc                    <  6:211813/149‑1 (MQ=255)
                                                                                                                                   aTGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTc                    <  3:173338/143‑1 (MQ=255)
                                                                                                                                   aTGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTc                    >  4:173338/1‑143 (MQ=255)
                                                                                                                                   aTGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTgg              >  4:125396/1‑149 (MQ=255)
                                                                                                                                         cTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCgg        >  4:202139/1‑149 (MQ=255)
                                                                                                                                            cAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCgggg      <  7:135048/148‑1 (MQ=255)
                                                                                                                                            cAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCggg       <  7:242395/147‑1 (MQ=255)
                                                                                                                                            cAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCggg       <  8:43332/147‑1 (MQ=255)
                                                                                                                                               gTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCGGGGTGaa  <  2:121230/149‑1 (MQ=255)
                                                                                                                                               gTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCGGGGTGaa  <  5:126368/149‑1 (MQ=255)
                                                                                                                                               gTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACACAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTTGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCGGGGTGaa  <  6:168339/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCCTTGTCTTGCCACTGGTGGCAAATGCGCAACCGGCTTCGGTGGTGTTTCTCAACCCGGGGCTGT‑‑‑CCACCGAGACATTCTGGACCAGCTATACCCGCTTCATGCAGGCCGCCGCGGACGAGCTGGGCATGACCCTGCAGGTGCAATATAGCGAGCGCCGCGCCGACCTGACACTGACCCAGGCCCGGGCGATCCTGAGCGCGCCGCAGCGGCCGGATTACCTGGTGCTGGTCAACGAGCAGTACGTGGCACCGGAAATCCTGCGCCTGTCGCGTGGCACCGGGGTGAA  >  NC_002947/2709450‑2709738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑