Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,710,330 G→T 15.2% V303V (GTG→GTT)  PP_2372 → sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,710,3300GT15.2% 177.2 / 21.2 66V303V (GTG→GTT) PP_2372sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base T (7/3);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

GTCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCT  >  NC_002947/2710187‑2710476
                                                                                                                                               |                                                                                                                                                  
gTCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTcgcg                                                                                                                                                >  5:212982/1‑148 (MQ=255)
 tCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGTGCGaa                                                                                                                                              >  3:60015/1‑149 (MQ=255)
 tCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGaa                                                                                                                                              <  1:325499/149‑1 (MQ=255)
        cTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTa                                                                                                                                       >  8:18866/1‑149 (MQ=255)
          cGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGTTGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcgc                                                                                                                                     <  6:212982/149‑4 (MQ=255)
           gCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTTACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTc                                                                                                                                    >  4:279349/1‑149 (MQ=255)
           gCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTc                                                                                                                                    >  3:131852/1‑149 (MQ=255)
            cccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTcc                                                                                                                                   >  4:191468/1‑149 (MQ=255)
             ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                  <  2:114121/149‑1 (MQ=255)
             ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                  <  2:77836/149‑1 (MQ=255)
              cGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCgg                                                                                                                                 <  2:262402/149‑1 (MQ=255)
               gAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGt                                                                                                                                <  8:156194/149‑1 (MQ=255)
                  gCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTAtt                                                                                                                             <  7:18866/149‑1 (MQ=255)
                     ttGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGTCGGCGTGCGCGAACGCCGTATTCCGGTATTg                                                                                                                            >  4:159473/1‑147 (MQ=255)
                       gcgcgcgcGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                          <  3:62527/147‑1 (MQ=255)
                       gcgcgcgcGTATCGATGGGCGCCTGAGCGTACTGATGGGGGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAg                                                                                                                         <  5:257624/148‑1 (MQ=255)
                        cgcgcgcgTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAgg                                                                                                                        <  1:13408/148‑1 (MQ=255)
                         gcgcgcgTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGaa                                                                                                                      <  5:312741/149‑1 (MQ=255)
                                        ggCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcg                                                                                                                                      <  8:86801/118‑3 (MQ=255)
                                        ggCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcg                                                                                                                                      >  7:86801/1‑116 (MQ=255)
                                        ggCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCaggccaa                                                                                                        <  1:87764/148‑1 (MQ=255)
                                            ccTGAGCGTACTGATGGTTGGGCATTTCAACCTTGGCGGTTGGTCCATGGTGATGCTTCACGATGATTCACACGCGCTGGCGTTGAACCGCGACGGCGTGAGCGAACGCATTATTCCCGTATTGCAGGAAATCCATCaggccaagtcca                                                                                                   >  4:272838/1‑149 (MQ=255)
                                             cTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAg                                                                                                  >  7:265349/1‑149 (MQ=255)
                                                aGCGTACTGATGGGGGGGCATTTCACCCGTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGc                                                                                                <  3:272838/148‑1 (MQ=255)
                                                   gTACTGTTGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTg                                                                                            <  4:131852/149‑1 (MQ=255)
                                                    tACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTgg                                                                                           <  1:13210/149‑1 (MQ=255)
                                                          tgggtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGTCGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAg                                                                                     <  3:159473/149‑1 (MQ=255)
                                                           gggtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGTGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAg                                                                                     <  4:60015/148‑1 (MQ=255)
                                                             gtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTg                                                                                  >  3:301329/1‑149 (MQ=255)
                                                              tgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGt                                                                                 >  1:32605/1‑149 (MQ=255)
                                                                ggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTg                                                                               >  8:111659/1‑149 (MQ=255)
                                                                        aCCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGga                                                                       >  1:177027/1‑149 (MQ=255)
                                                                          ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                      <  2:325115/148‑1 (MQ=255)
                                                                          ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                      >  1:325115/1‑148 (MQ=255)
                                                                          ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                      >  8:124145/1‑148 (MQ=255)
                                                                                cGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACg                                                               <  2:177027/149‑1 (MQ=255)
                                                                                   ttGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCg                                                            >  1:174861/1‑149 (MQ=255)
                                                                                       gCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGa                                                        >  8:154477/1‑149 (MQ=255)
                                                                                         cATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtt                                                      >  6:68411/1‑149 (MQ=255)
                                                                                           tGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtttt                                                    <  3:191468/149‑1 (MQ=255)
                                                                                               gATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGc                                                <  8:265349/149‑1 (MQ=255)
                                                                                                   ctgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                            <  7:154477/149‑1 (MQ=255)
                                                                                                     gcAAGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                            >  5:303557/1‑147 (MQ=255)
                                                                                                      cACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACa                                          <  2:174861/148‑1 (MQ=255)
                                                                                                        cGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTg                                       >  2:105236/1‑149 (MQ=255)
                                                                                                         gatgatGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGc                                      >  4:110512/1‑149 (MQ=255)
                                                                                                             atgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCg                                    >  3:33246/1‑147 (MQ=255)
                                                                                                             atgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGaa                                  <  3:110512/149‑1 (MQ=255)
                                                                                                                cccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAgg                                <  5:68411/148‑1 (MQ=255)
                                                                                                                 ccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggc                              >  5:233884/1‑149 (MQ=255)
                                                                                                                  cACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcg                             >  3:175423/1‑149 (MQ=255)
                                                                                                                           gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                    <  7:111659/149‑1 (MQ=255)
                                                                                                                           gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCg                     <  3:279349/148‑1 (MQ=255)
                                                                                                                             gTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCggg                   >  2:174963/1‑148 (MQ=255)
                                                                                                                                 ccggaataCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCg              >  3:271050/8‑149 (MQ=255)
                                                                                                                                 aaCCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCg              >  4:3736/1‑149 (MQ=255)
                                                                                                                                  cggaataCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGt             >  1:332770/7‑149 (MQ=255)
                                                                                                                                  aCCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGt             >  6:203395/1‑149 (MQ=255)
                                                                                                                                       taCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc        >  8:138077/2‑149 (MQ=255)
                                                                                                                                       taCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTAAGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc        >  4:299182/2‑149 (MQ=255)
                                                                                                                                         cGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGt      >  5:318905/1‑149 (MQ=255)
                                                                                                                                         cGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGt      <  4:271050/149‑1 (MQ=255)
                                                                                                                                          ggCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGtt     <  6:233884/149‑1 (MQ=255)
                                                                                                                                            cGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTTGGCGTTACAGTGCCGCAGGGCGGCCGCCCGGGTACCGTTACCCGTTTc   >  3:95522/1‑149 (MQ=255)
                                                                                                                                            cGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTc   >  3:5765/1‑149 (MQ=255)
                                                                                                                                             gTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCt  >  2:26118/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GTCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCT  >  NC_002947/2710187‑2710476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑