Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,710,335 A→C 13.9% E305A (GAA→GCA)  PP_2372 → sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,710,3350AC13.9% 168.4 / 18.1 64E305A (GAA→GCA) PP_2372sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (28/27);  new base C (6/3);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTC  >  NC_002947/2710188‑2710482
                                                                                                                                                   |                                                                                                                                                   
tCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGTGCGaa                                                                                                                                                    >  3:60015/1‑149 (MQ=255)
tCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGaa                                                                                                                                                    <  1:325499/149‑1 (MQ=255)
       cTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTa                                                                                                                                             >  8:18866/1‑149 (MQ=255)
         cGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGTTGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcgc                                                                                                                                           <  6:212982/149‑4 (MQ=255)
          gCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTTACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTc                                                                                                                                          >  4:279349/1‑149 (MQ=255)
          gCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTc                                                                                                                                          >  3:131852/1‑149 (MQ=255)
           cccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTcc                                                                                                                                         >  4:191468/1‑149 (MQ=255)
            ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                        <  2:77836/149‑1 (MQ=255)
            ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                        <  2:114121/149‑1 (MQ=255)
             cGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCgg                                                                                                                                       <  2:262402/149‑1 (MQ=255)
              gAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGt                                                                                                                                      <  8:156194/149‑1 (MQ=255)
                 gCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTAtt                                                                                                                                   <  7:18866/149‑1 (MQ=255)
                    ttGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGTCGGCGTGCGCGAACGCCGTATTCCGGTATTg                                                                                                                                  >  4:159473/1‑147 (MQ=255)
                      gcgcgcgcGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                                <  3:62527/147‑1 (MQ=255)
                      gcgcgcgcGTATCGATGGGCGCCTGAGCGTACTGATGGGGGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAg                                                                                                                               <  5:257624/148‑1 (MQ=255)
                       cgcgcgcgTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAgg                                                                                                                              <  1:13408/148‑1 (MQ=255)
                        gcgcgcgTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGaa                                                                                                                            <  5:312741/149‑1 (MQ=255)
                                       ggCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcg                                                                                                                                            <  8:86801/118‑3 (MQ=255)
                                       ggCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcg                                                                                                                                            >  7:86801/1‑116 (MQ=255)
                                       ggCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCaggccaa                                                                                                              <  1:87764/148‑1 (MQ=255)
                                           ccTGAGCGTACTGATGGTTGGGCATTTCAACCTTGGCGGTTGGTCCATGGTGATGCTTCACGATGATTCACACGCGCTGGCGTTGAACCGCGACGGCGTGAGCGAACGCATTATTCCCGTATTGCAGGAAATCCATCaggccaagtcca                                                                                                         >  4:272838/1‑149 (MQ=255)
                                            cTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAg                                                                                                        >  7:265349/1‑149 (MQ=255)
                                               aGCGTACTGATGGGGGGGCATTTCACCCGTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGc                                                                                                      <  3:272838/148‑1 (MQ=255)
                                                  gTACTGTTGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTg                                                                                                  <  4:131852/149‑1 (MQ=255)
                                                   tACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTgg                                                                                                 <  1:13210/149‑1 (MQ=255)
                                                         tgggtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGTCGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAg                                                                                           <  3:159473/149‑1 (MQ=255)
                                                          gggtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGTGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAg                                                                                           <  4:60015/148‑1 (MQ=255)
                                                            gtgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTg                                                                                        >  3:301329/1‑149 (MQ=255)
                                                             tgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGt                                                                                       >  1:32605/1‑149 (MQ=255)
                                                               ggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTg                                                                                     >  8:111659/1‑149 (MQ=255)
                                                                       aCCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGga                                                                             >  1:177027/1‑149 (MQ=255)
                                                                         ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                            >  8:124145/1‑148 (MQ=255)
                                                                         ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                            >  1:325115/1‑148 (MQ=255)
                                                                         ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                            <  2:325115/148‑1 (MQ=255)
                                                                               cGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACg                                                                     <  2:177027/149‑1 (MQ=255)
                                                                                  ttGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCg                                                                  >  1:174861/1‑149 (MQ=255)
                                                                                      gCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGa                                                              >  8:154477/1‑149 (MQ=255)
                                                                                        cATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtt                                                            >  6:68411/1‑149 (MQ=255)
                                                                                          tGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtttt                                                          <  3:191468/149‑1 (MQ=255)
                                                                                              gATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGc                                                      <  8:265349/149‑1 (MQ=255)
                                                                                                  ctgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                  <  7:154477/149‑1 (MQ=255)
                                                                                                    gcAAGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                  >  5:303557/1‑147 (MQ=255)
                                                                                                     cACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACa                                                <  2:174861/148‑1 (MQ=255)
                                                                                                       cGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTg                                             >  2:105236/1‑149 (MQ=255)
                                                                                                        gatgatGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGc                                            >  4:110512/1‑149 (MQ=255)
                                                                                                            atgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCg                                          >  3:33246/1‑147 (MQ=255)
                                                                                                            atgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGaa                                        <  3:110512/149‑1 (MQ=255)
                                                                                                               cccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAgg                                      <  5:68411/148‑1 (MQ=255)
                                                                                                                ccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggc                                    >  5:233884/1‑149 (MQ=255)
                                                                                                                 cACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcg                                   >  3:175423/1‑149 (MQ=255)
                                                                                                                          gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                          <  7:111659/149‑1 (MQ=255)
                                                                                                                          gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCg                           <  3:279349/148‑1 (MQ=255)
                                                                                                                            gTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCggg                         >  2:174963/1‑148 (MQ=255)
                                                                                                                                ccggaataCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCg                    >  3:271050/8‑149 (MQ=255)
                                                                                                                                aaCCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCg                    >  4:3736/1‑149 (MQ=255)
                                                                                                                                 cggaataCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGt                   >  1:332770/7‑149 (MQ=255)
                                                                                                                                 aCCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGt                   >  6:203395/1‑149 (MQ=255)
                                                                                                                                      taCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc              >  8:138077/2‑149 (MQ=255)
                                                                                                                                      taCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTAAGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc              >  4:299182/2‑149 (MQ=255)
                                                                                                                                        cGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGt            >  5:318905/1‑149 (MQ=255)
                                                                                                                                        cGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGt            <  4:271050/149‑1 (MQ=255)
                                                                                                                                         ggCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGtt           <  6:233884/149‑1 (MQ=255)
                                                                                                                                           cGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTTGGCGTTACAGTGCCGCAGGGCGGCCGCCCGGGTACCGTTACCCGTTTc         >  3:95522/1‑149 (MQ=255)
                                                                                                                                           cGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTc         >  3:5765/1‑149 (MQ=255)
                                                                                                                                            gTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCt        >  2:26118/1‑149 (MQ=255)
                                                                                                                                                  gAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTc  <  2:32605/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCGTACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTC  >  NC_002947/2710188‑2710482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑