Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,799,177 C→T 13.4% intergenic (‑191/+25) PP_2452 ← / ← ansB hypothetical protein/glutaminase‑asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,799,1770CT13.4% 122.4 / 9.9 52intergenic (‑191/+25)PP_2452/ansBhypothetical protein/glutaminase‑asparaginase
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base T (5/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.58e-01

AGTAAATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTT  >  NC_002947/2799034‑2799321
                                                                                                                                                |                                                                                                                                                
agtaaATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCAcc                                                                                                                                             >  5:134582/1‑149 (MQ=255)
agtaaATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCAcc                                                                                                                                             >  2:271412/1‑149 (MQ=255)
     aTTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGccc                                                                                                                                        >  5:267615/1‑149 (MQ=255)
         gCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGa                                                                                                                                     <  5:18077/148‑1 (MQ=255)
         gCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAg                                                                                                                                    >  8:34153/1‑149 (MQ=255)
          cAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                   <  7:34153/149‑1 (MQ=255)
          cAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                   >  2:5691/1‑149 (MQ=255)
           aaCAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAggg                                                                                                                                  >  7:242067/1‑149 (MQ=255)
            aCAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACATCTGTGTCTACGC‑GGTTCACCTTATGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgggg                                                                                                                                 >  1:284128/1‑149 (MQ=255)
              aGCTTGTGAGTAGAAAACCAGCGTGGGCCTGTAAACCATGAGGCCGGGCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGa                                                                                                                               <  4:234828/149‑1 (MQ=255)
              aGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAggggg                                                                                                                                >  8:174192/1‑148 (MQ=255)
              aGCATGTCACGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGGCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGACGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGa                                                                                                                               <  4:239149/149‑1 (MQ=255)
                   gTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCa                                                                                                                           <  1:5691/148‑1 (MQ=255)
                        gagaAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCACGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAg                                                                                                                      <  1:271412/149‑1 (MQ=255)
                           aaaTCCAGCGGTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGGCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACt                                                                                                                  <  1:216182/149‑1 (MQ=255)
                                        gCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCg                                                                                                     <  6:134582/149‑1 (MQ=255)
                                             tAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTg                                                                                                  <  6:143742/147‑1 (MQ=255)
                                                aCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGccc                                                                                                                                        >  5:341557/1‑106 (MQ=255)
                                                aCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGccc                                                                                                                                        <  6:341557/106‑1 (MQ=255)
                                                aCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGct                                                                                             >  3:58283/1‑149 (MQ=255)
                                                    tGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCttt                                                                                         >  3:65774/1‑149 (MQ=255)
                                                      aGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                        <  2:196741/148‑1 (MQ=255)
                                                               aGAAAGCTAAACGATGTCGCCGGTTTGTACCCTCTGTGTCTACGC‑GGTTGAGGATTTGGCAACGAGGCCGTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCTCTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTggg                                                                              <  7:223367/149‑1 (MQ=255)
                                                                gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGATGCAGCTCTTTGCTGTCCTGGGt                                                                             >  7:114534/1‑149 (MQ=255)
                                                                 aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                               <  1:215128/146‑1 (MQ=255)
                                                                 aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                               >  2:215128/1‑146 (MQ=255)
                                                                 aaaGGTAAACGATGTAGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTc                                                                            >  8:78840/1‑149 (MQ=255)
                                                                     gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                        <  4:58283/149‑1 (MQ=255)
                                                                     gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                        <  4:65774/149‑1 (MQ=255)
                                                                     gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                        >  3:200191/1‑149 (MQ=255)
                                                                     gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                        >  3:303977/1‑149 (MQ=255)
                                                                      tAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGAAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGt                                                                       >  3:74745/1‑149 (MQ=255)
                                                                         aCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGAACCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCAt                                                                    >  4:15994/1‑149 (MQ=255)
                                                                                     gTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGc                                                        <  8:114534/149‑1 (MQ=255)
                                                                                                  gtgtCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCt                                           >  4:37509/1‑149 (MQ=255)
                                                                                                        aCGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                     >  3:120313/1‑149 (MQ=255)
                                                                                                         cgc‑gGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                     <  4:200191/148‑1 (MQ=255)
                                                                                                          gc‑gGTTGAGGAATTGGCACCGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                   <  7:78840/149‑1 (MQ=255)
                                                                                                                 gAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTACTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAg                             >  4:212249/1‑149 (MQ=255)
                                                                                                                   ggAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTACTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAgg                            <  3:212249/148‑1 (MQ=255)
                                                                                                                         ggCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                      <  5:315430/148‑1 (MQ=255)
                                                                                                                         ggCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                      <  7:40566/148‑1 (MQ=255)
                                                                                                                         ggCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGAAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                      <  4:74745/148‑1 (MQ=255)
                                                                                                                                  ccccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAcc             >  3:139028/2‑148 (MQ=255)
                                                                                                                                    ccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg          >  4:155797/1‑149 (MQ=255)
                                                                                                                                    ccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg          >  4:88053/1‑149 (MQ=255)
                                                                                                                                    ccTCAGAGCCGGCGCACCGGCCCTGAGTGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGGCTCACGAcctga          <  2:249602/149‑4 (MQ=255)
                                                                                                                                     cTCAGGGCCGGTGCGCCGGCTCTGAGGGTGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                   <  8:226873/123‑1 (MQ=255)
                                                                                                                                     cTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCACAGAAGATACGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                   >  7:226873/1‑123 (MQ=255)
                                                                                                                                         gggCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGtt     >  2:289854/3‑149 (MQ=255)
                                                                                                                                          agCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTc    <  4:120313/149‑1 (MQ=255)
                                                                                                                                            ccGTCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCtt  <  4:303977/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGTAAATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTT  >  NC_002947/2799034‑2799321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑