Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,799,186 C→T 14.6% intergenic (‑200/+16) PP_2452 ← / ← ansB hypothetical protein/glutaminase‑asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,799,1860CT14.6% 112.0 / 10.1 48intergenic (‑200/+16)PP_2452/ansBhypothetical protein/glutaminase‑asparaginase
Reads supporting (aligned to +/- strand):  ref base C (17/24);  new base T (5/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-01

ATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGT  >  NC_002947/2799039‑2799334
                                                                                                                                                    |                                                                                                                                                    
aTTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGccc                                                                                                                                                     >  5:267615/1‑149 (MQ=255)
    gCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGa                                                                                                                                                  <  5:18077/148‑1 (MQ=255)
    gCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAg                                                                                                                                                 >  8:34153/1‑149 (MQ=255)
     cAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                                <  7:34153/149‑1 (MQ=255)
     cAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                                >  2:5691/1‑149 (MQ=255)
      aaCAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAggg                                                                                                                                               >  7:242067/1‑149 (MQ=255)
       aCAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACATCTGTGTCTACGC‑GGTTCACCTTATGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgggg                                                                                                                                              >  1:284128/1‑149 (MQ=255)
         aGCTTGTGAGTAGAAAACCAGCGTGGGCCTGTAAACCATGAGGCCGGGCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGa                                                                                                                                            <  4:234828/149‑1 (MQ=255)
         aGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAggggg                                                                                                                                             >  8:174192/1‑148 (MQ=255)
         aGCATGTCACGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGGCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGACGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGa                                                                                                                                            <  4:239149/149‑1 (MQ=255)
              gTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCa                                                                                                                                        <  1:5691/148‑1 (MQ=255)
                   gagaAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCACGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAg                                                                                                                                   <  1:271412/149‑1 (MQ=255)
                      aaaTCCAGCGGTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGGCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACt                                                                                                                               <  1:216182/149‑1 (MQ=255)
                                   gCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCg                                                                                                                  <  6:134582/149‑1 (MQ=255)
                                        tAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTg                                                                                                               <  6:143742/147‑1 (MQ=255)
                                           aCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGccc                                                                                                                                                     <  6:341557/106‑1 (MQ=255)
                                           aCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGccc                                                                                                                                                     >  5:341557/1‑106 (MQ=255)
                                           aCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGct                                                                                                          >  3:58283/1‑149 (MQ=255)
                                               tGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCttt                                                                                                      >  3:65774/1‑149 (MQ=255)
                                                 aGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                                     <  2:196741/148‑1 (MQ=255)
                                                          aGAAAGCTAAACGATGTCGCCGGTTTGTACCCTCTGTGTCTACGC‑GGTTGAGGATTTGGCAACGAGGCCGTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCTCTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTggg                                                                                           <  7:223367/149‑1 (MQ=255)
                                                           gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGATGCAGCTCTTTGCTGTCCTGGGt                                                                                          >  7:114534/1‑149 (MQ=255)
                                                            aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                            >  2:215128/1‑146 (MQ=255)
                                                            aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                            <  1:215128/146‑1 (MQ=255)
                                                            aaaGGTAAACGATGTAGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTc                                                                                         >  8:78840/1‑149 (MQ=255)
                                                                gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                                     <  4:58283/149‑1 (MQ=255)
                                                                gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                                     <  4:65774/149‑1 (MQ=255)
                                                                gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                                     >  3:200191/1‑149 (MQ=255)
                                                                gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                                     >  3:303977/1‑149 (MQ=255)
                                                                 tAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGAAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGt                                                                                    >  3:74745/1‑149 (MQ=255)
                                                                    aCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGAACCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCAt                                                                                 >  4:15994/1‑149 (MQ=255)
                                                                                gTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGc                                                                     <  8:114534/149‑1 (MQ=255)
                                                                                             gtgtCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCt                                                        >  4:37509/1‑149 (MQ=255)
                                                                                                   aCGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                                  >  3:120313/1‑149 (MQ=255)
                                                                                                    cgc‑gGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                                  <  4:200191/148‑1 (MQ=255)
                                                                                                     gc‑gGTTGAGGAATTGGCACCGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                                <  7:78840/149‑1 (MQ=255)
                                                                                                            gAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTACTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAg                                          >  4:212249/1‑149 (MQ=255)
                                                                                                              ggAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTACTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAgg                                         <  3:212249/148‑1 (MQ=255)
                                                                                                                    ggCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                                   <  5:315430/148‑1 (MQ=255)
                                                                                                                    ggCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                                   <  7:40566/148‑1 (MQ=255)
                                                                                                                    ggCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGAAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                                   <  4:74745/148‑1 (MQ=255)
                                                                                                                             ccccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAcc                          >  3:139028/2‑148 (MQ=255)
                                                                                                                               ccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg                       >  4:155797/1‑149 (MQ=255)
                                                                                                                               ccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg                       >  4:88053/1‑149 (MQ=255)
                                                                                                                               ccTCAGAGCCGGCGCACCGGCCCTGAGTGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGGCTCACGAcctga                       <  2:249602/149‑4 (MQ=255)
                                                                                                                                cTCAGGGCCGGTGCGCCGGCTCTGAGGGTGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                                <  8:226873/123‑1 (MQ=255)
                                                                                                                                cTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCACAGAAGATACGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                                >  7:226873/1‑123 (MQ=255)
                                                                                                                                    gggCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGtt                  >  2:289854/3‑149 (MQ=255)
                                                                                                                                     agCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTc                 <  4:120313/149‑1 (MQ=255)
                                                                                                                                       ccGTCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCtt               <  4:303977/149‑1 (MQ=255)
                                                                                                                                             cACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATc         <  6:267615/149‑1 (MQ=255)
                                                                                                                                                    ccTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGt  >  3:232702/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGC‑GGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGT  >  NC_002947/2799039‑2799334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑