Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,810,342	T→C	6.2%	L250P (CTC→CCC)	thrS →	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,810,342	0	T	C	6.2%	185.6 / 2.1	64	L250P (CTC→CCC)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (28/32);  new base C (2/2);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CGGCGCCTACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTG  >  NC_002947/2810196‑2810485                                                                                                                                                    |                                                                                                                                                 cGGCGCCTACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTc                                                                                                                                                 <  5:242820/148‑1 (MQ=255) cGGCGCCTACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCg                                                                                                                                                >  5:56376/1‑149 (MQ=255) cGGCGCCTACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCg                                                                                                                                                <  5:212154/149‑1 (MQ=255)   gcgcCTACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGAc                                                                                                                                              >  1:124043/1‑149 (MQ=255)    cgcCTACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGAcc                                                                                                                                             <  4:282401/149‑1 (MQ=255)        tACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGtt                                                                                                                                         >  5:32630/1‑149 (MQ=255)             gcgtggcgATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCAc                                                                                                                                     <  6:105085/148‑1 (MQ=255)               gtggcgATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTg                                                                                                                                  <  8:304881/149‑1 (MQ=255)                  gcgATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCa                                                                                                                                <  6:32630/148‑1 (MQ=255)                        ccAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGgaag                                                                                                                           >  2:310535/1‑147 (MQ=255)                        ccAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGAATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGgaagaa                                                                                                                         >  6:168557/1‑149 (MQ=255)                         cAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGaagaag                                                                                                                        >  6:297389/1‑149 (MQ=255)                         cAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGaagaag                                                                                                                        >  2:64062/1‑149 (MQ=255)                          aaGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGc                                                                                                                       <  7:306865/149‑1 (MQ=255)                             aaCGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGcccc                                                                                                                    <  2:124043/149‑1 (MQ=255)                                   cagcTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGc                                                                                                                                 <  4:325051/130‑1 (MQ=255)                                   cagcTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGc                                                                                                                                 >  3:325051/1‑130 (MQ=255)                                    agcTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATg                                                                                                             <  3:83262/149‑1 (MQ=255)                                      cTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑gt                                                                                                          >  1:68402/1‑149 (MQ=255)                                        tCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑gtgt                                                                                                        >  8:197418/1‑149 (MQ=255)                                          aGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTc                                                                                                      <  5:168557/149‑1 (MQ=255)                                            cgcgTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCCCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTg                                                                                                    >  6:200178/1‑149 (MQ=255)                                            cgcgTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCCCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTg                                                                                                    >  8:46583/1‑149 (MQ=255)                                              cgTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGc                                                                                                  <  5:14773/149‑1 (MQ=255)                                               gtgtACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCa                                                                                                 <  1:310535/149‑1 (MQ=255)                                                tgtACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCCCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCAc                                                                                                <  5:200178/149‑1 (MQ=255)                                                tgtACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCCCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCAc                                                                                                <  7:46583/149‑1 (MQ=255)                                                  tACGGCACGGGCTGGGCTGACAAGCAGCAGGTTGCTGCGTACATCCATCGCATCCAAGAAGCCGTAAAACGCGCCC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACg                                                                                               <  1:149778/148‑1 (MQ=255)                                                          ggcCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGAc                                                                                                                                              <  2:221196/94‑1 (MQ=255)                                                          ggcCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGAc                                                                                                                                              >  1:221196/1‑94 (MQ=255)                                                          ggcCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCt                                                                                      <  1:64062/149‑1 (MQ=255)                                                                ggCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGAcc                                                                                 <  4:150497/148‑1 (MQ=255)                                                                 gCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGt                                                                               >  2:113684/1‑149 (MQ=255)                                                                   tGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTc                                                                              >  3:200976/1‑148 (MQ=255)                                                                       aagaagCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCGACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACGTCCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGCCTGGCCCGTCTccc                                                                          >  1:93787/1‑149 (MQ=255)                                                                         gaagCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAgg                                                                       >  4:315976/1‑149 (MQ=255)                                                                              agcTGGCTGCGTACATGCAGCGCATCGAAGATGCCGAAAAACGCGTCG‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTc                                                                  <  6:56376/149‑1 (MQ=255)                                                                              agcTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTc                                                                  >  2:47062/1‑149 (MQ=255)                                                                                  ggctgCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAg                                                               >  8:143451/1‑148 (MQ=255)                                                                                   gctgCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAg                                                               <  1:47062/147‑1 (MQ=255)                                                                                       cGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTAc                                                         <  2:93787/149‑1 (MQ=255)                                                                                        gTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACa                                                        >  7:230782/1‑149 (MQ=255)                                                                                                     aTCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCa                                           <  8:230782/149‑1 (MQ=255)                                                                                                     aTCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCa                                           <  7:197418/149‑1 (MQ=255)                                                                                                          agaagCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCa                                           >  4:242000/1‑144 (MQ=255)                                                                                                          agaagCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCa                                           <  3:242000/144‑1 (MQ=255)                                                                                                           ccgtctgAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAgcgc                                       <  3:193672/141‑1 (MQ=255)                                                                                                              gCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGaaaa                                  >  3:293209/1‑149 (MQ=255)                                                                                                                 gAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACgg                               >  5:135703/1‑149 (MQ=255)                                                                                                                   aaaaCGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAACCGGCt                             <  2:68402/149‑1 (MQ=255)                                                                                                                   aaaaCGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCt                             <  4:200976/149‑1 (MQ=255)                                                                                                                      aCGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGAAAGTTAATGCACGCCAACGGCATGACCGTAAACCAGGTAATAGACCAGTACATGCGTGGCGTGCAGCGCCAAAACgggaac                           >  1:106115/1‑145 (MQ=255)                                                                                                                      aCGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTAcc                          <  1:135889/149‑1 (MQ=255)                                                                                                                       cgcgACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTAc                           >  4:165380/1‑147 (MQ=255)                                                                                                                        gcgACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTa                            >  3:132045/1‑145 (MQ=255)                                                                                                                        gcgACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTa                            <  4:132045/145‑1 (MQ=255)                                                                                                                        gcgACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAg                        <  5:297389/149‑1 (MQ=255)                                                                                                                            cc‑accGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGaaa                    <  1:14034/149‑1 (MQ=255)                                                                                                                               accGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGTTGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATc                  <  1:113684/149‑1 (MQ=255)                                                                                                                               accGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAAt                   >  6:115554/1‑148 (MQ=255)                                                                                                                                ccGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATc                  >  6:90684/1‑148 (MQ=255)                                                                                                                                   cAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGa              <  3:165380/149‑1 (MQ=255)                                                                                                                                        tCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCg         >  5:123925/1‑149 (MQ=255)                                                                                                                                               gcagcTCGACCTGTTCCAGCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTg  >  8:84135/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CGGCGCCTACTGGCGTGGCGATGCCAAGAACGAGCAGCTTCAGCGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACC‑ACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATG‑GTGTTCTGGCACGCCAACGGCTGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTG  >  NC_002947/2810196‑2810485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑